Reduced miR-203 predicts metastasis and poor survival in esophageal carcinoma

He, Rongqi; Wang, Jintian; Yang, Kai; Du, Jiabin; Chen, Junxing; Liu, Weinan

doi:10.18632/aging.102543

Cited by 15 publications

(13 citation statements)

References 37 publications

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“…The goal of our re-analysis was to exclusively represent relative expression of miR-203 in EC tissue and tumor-adjacent normal tissue. Confirming the previous findings [ 24 ], in 119 paired patient samples in GSE43732, miR-203 expression was significantly downregulated in esophageal tumor tissue (Fig. 1 c; P < 0.0001) and in 104 tumor-adjacent normal tissue and 153 esophageal tumor tissue patient samples in GSE6188, miR-203 expression was significantly downregulated in esophageal tumor tissue (Fig.…”

Section: Resultssupporting

confidence: 88%

“…Obviously, USP26 is not the only target of miR-203a-5p. In the context of EC, miR-203a's function has been to inhibit β-catenin signaling, cell migration and invasion via directly inhibiting KIF5C expression in turn potentiating the antitumor activities of the downstream protein, Axin2 [24]. Different targets of miR-203a have been defined in the context of other cancers, like RAB22A (encoding Rasrelated protein Rab-22A) and BIRC5 (encoding survivin) in osteosarcoma [27,28].…”

Section: Discussionmentioning

confidence: 99%

“…We next analyzed miR-203 expression in GSE43732 and GSE6188. It is important to note that previous analysis of these 2 datasets by He et al 2019 [24] had shown miR-203 as one of 17 differentially expressed miRNA between EC and tumor-adjacent normal tissue in patient samples. The goal of our re-analysis was to exclusively represent relative expression of miR-203 in EC tissue and tumor-adjacent normal tissue.…”

Section: Differential Regulation Of Snai1 and Hsa-mir-203 In Esophagementioning

confidence: 97%

“…Much like the protein effectors of EMT and metastatic progression, several microRNAs (miRNAs) have been indicated to be critical in EMT and metastatic progression [ 19 – 23 ]. Among the different miRNAs that have been shown to regulate different steps of EC, there seems to be a consensus about the miR-203 functioning as an inhibitor of metastatic progression in EC [ 24 ] with its expression downregulated in EC. It has been reported that miR-203 inhibits EC progression by targeting the stem cell renewal factor Bmi-1 [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1’s pro-metastatic functions in esophageal cancer

Dong

et al. 2020

Cancer Cell Int

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Background: Esophageal cancer is one of the most common cancers worldwide with poor prognosis and high mortality. The transcription factor SNAI1, encoding Snail1, is important for metastatic progression in esophageal cancer whereas the microRNA (miRNA)-203 has been shown to function as an inhibitor of metastasis in EC. The Snail1 protein is stabilized in EC partially by the deubiquitinating enzyme USP26; however, how USP26 is regulated is not completely known. Methods: Expression of SNAI1 and USP26 messenger RNA (mRNA) and miR-203 was performed in datasets within The Cancer Genome Atlas and Gene Expression Omnibus, respectively. Expression of Snail1 and USP26 protein and miR-203 was determined in the normal esophageal cell line HET-1A and EC cell lines Kyse150 and TE-1 using western blot and quantitative polymerase chain reaction, respectively. TargetScan was used for in situ prediction of miR-203 targets and in vitro heterologous reporter assays using the wild-type and miR-203 seed mutant of the 3′ Untranslated region (UTR) of USP26 were used to investigate whether USP26 is a target of miR-203. Effects of increasing miR-203 using MIR203A/5P mimic on USP26 and Snail1 in the HET-1A, Kyse150 and TE-1 cell lines were performed using western blot and cycloheximide-based protein stability analysis. Effects of modulating miR-203 in Kyse150 and TE-1 cell lines on in vitro pro-metastatic effects were analyzed by invasion assay, scratch wound-healing assay, and chemosensitivity to 5-fluoruracil (5-FU). In vivo lung metastasis assay was used to study the effect of modulating miR-203 in Kyse150 cells. Results: SNAI1 mRNA and HSA/MIR203 was higher and lower, respectively, in EC patients compared to tumoradjacent normal tissues. No changes in expression of USP26 mRNA were observed in these datasets. MIR/203 expression was downregulated whereas protein expression of both Snail1 and USP26 were higher in EC cell lines Kyse150 and TE-1 compared to normal esophageal cell line HET-1A. USP26 was predicted as a potential target of miR-203 by TargetScan Release 2.0. Reporter assays confirmed USP26 as a target of miR-203 in the EC cell lines. Transfection of EC cell lines with MIR203 mimic decreased USP26 protein expression and Snail1 protein stability indicating the ability of miR-203 to regulate Snail1 protein levels via USP26. Exogenous increase in miR-203 in the EC cell lines significantly inhibited Snail-1 mediated in vitro pro-metastatic function of invasion, wound-healing, and increased chemosensitivity to 5-FU. Finally, overexpression of miR-203 inhibited in vivo lung metastasis of Kyse150 cells, which was reversed

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Section: Resultssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Differential Regulation Of Snai1 and Hsa-mir-203 In Esophagementioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1’s pro-metastatic functions in esophageal cancer

Dong

et al. 2020

Cancer Cell Int

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“…miR-203 is one of the important members of the miRNAs family, and located on human chromosome 14 q32, 33-site (17). Studies have shown that miR-203 is abnormally expressed in a variety of tumor tissues including esophageal carcinoma (down-regulation), osteosarcoma (down-regulation), ovarian cancer (upregulation), and prostate cancer (down-regulation), and is closely related to tumor cell growth, metastasis and invasion (18)(19)(20)(21)(22)(23). A recent study reported that miRNA-203 restrains epithelial-mesenchymal transition, invasion and migration of papillary thyroid cancer via downregulating AKT3 expression (24).…”

Section: Introductionmentioning

confidence: 99%

The Inhibition of Long Non-Coding RNA CAR10 and The Regulation of miR-203 Expression Suppresses Cell Viability and Induces Cell Apoptosis in Prostate Cancer

Zhang¹,

Chen²,

Wang³

et al. 2020

Preprint

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Background: Prostate cancer (PC) is one of the most common malignant tumors. Recently, it has been reported that long noncoding RNAs (lncRNAs) play key roles in tumor progression. Studies have revealed that long non-coding RNA CAR10 (CAR10) can regulate tumor cell behaviors through sponging miR-203. In this study, we examined the effects of CAR10 in PC cells. Methods: Firstly, real time-quantitative polymerase chain reaction (qRT-PCR) was used to explore CAR10 expression in tumor tissues, peripheral blood of PC patients, and PC cells. We used the dual-luciferase reporter gene assay to analyze the relationship between CAR10 and miR-203. Moreover, flow cytometry, MTT assay, and western blot assay were used to determine cell apoptosis, cell viability, and apoptosis-related protein expression. Results: The results showed that CAR10 expression was remarkably higher in PC samples compared with that of control, and CAR10 regulated miR-203 negatively in PC cells. The qRT-PCR results also showed that miR-203 expression was significantly decreased in PC samples. Moreover, knockdown of CAR10 inhibited PC cell viability and promoted cleaved caspase-3 expression but induced PC cell apoptosis and, reduced pro-caspase-3 expression; miR-203 inhibitor reversed these effects. Conclusion: Our study found that CAR10 is a potential oncogene in PC and suggests that CAR10 inhibition could inhibit PC cell viability but promote PC cell apoptosis through regulating miR-203 expression. Our results show that CAR10 is a potential target for the treatment of PC.

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LncRNA ZNF667-AS1 Targets miR-523-3p/KIF5C Axis to Hinder Colon Cancer Progression

Tong

Fan

2023

Mol Biotechnol

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Reduced miR-203 predicts metastasis and poor survival in esophageal carcinoma

Cited by 15 publications

References 37 publications

Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1’s pro-metastatic functions in esophageal cancer

Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1’s pro-metastatic functions in esophageal cancer

The Inhibition of Long Non-Coding RNA CAR10 and The Regulation of miR-203 Expression Suppresses Cell Viability and Induces Cell Apoptosis in Prostate Cancer

LncRNA ZNF667-AS1 Targets miR-523-3p/KIF5C Axis to Hinder Colon Cancer Progression

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