Reduced miR-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein

Li, Bao Hua; Zhou, Juxiang; Ye, Feng; Cheng, Xiuwen; Zhou, Cai Yun; Lü, Wei; Xie, Xing

doi:10.1016/j.ejca.2011.04.037

Cited by 93 publications

(78 citation statements)

References 39 publications

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“…For example, miR-100 expression is downregulated in lung, cervical, ovarian and bladder cancer, and acts as a tumor suppressor (26)(27)(28). However, in various types of cancer, such as acute myeloid leukemia, miR-100 is upregulated and functions as an oncogene (29).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of tumor suppressor and oncogenic miRNAs in gastric cancer

Chen

Liu

et al. 2015

Oncology Letters

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Abstract. The aim of the present study was to screen for and identify microRNAs (miRNAs/miRs) that are associated with gastric cancer and to clarify the role of these miRNAs in gastric cancer. Thus, the differential expression of a panel of miRNAs in two pairs of gastric cancer tissues and their matched adjacent healthy tissues was investigated by performing a microarray analysis. To verify the results of this screen, 56 gastric cancer tissues were analyzed for the selected miRNAs using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The association between the expression of a specific miRNA and the clinical data relating to the tissue samples [including age, gender, tumor size, tumor node metastasis (TNM) stage and lymph-node metastasis] were subsequently examined. A total of 31 differentially expressed miRNAs were identified in the miRNA array. Using RT-qPCR to verify these results, it was determined that 10 miRNAs exhibited high mRNA expression levels and 13 miRNAs exhibited a low expression in the gastric cancer tissue samples, while 8 miRNAs did not demonstrate an association with gastric cancer. Thus, the microarray and RT-qPCR results demonstrated 74.2% (23/31 miRNAs) agreement. The association between the 23 miRNAs and the clinicopathological characteristics of the gastric cancer samples was investigated. It was identified that the expression levels of miR-551b-3p, miR-133b, miR-100-5p and miR-363-3p were significantly downregulated in the gastric cancer tissues, and this downregulation was closely correlated with the degree of differentiation (i.e., tumor grade), TNM stage and lymph-node metastasis (P<0.05). By contrast, the expression of miR-215 was significantly upregulated in the gastric cancer tissues, and its expression level was correlated with tumor differentiation, TNM stage and lymph-node metastasis (P<0.05). Furthermore, miR-200a-3p was upregulated in the gastric cancer tissues and its expression was significantly more prevalent in male patients compared with female patients (P<0.05). miR-429 was upregulated in the gastric cancer tissues and its expression was significantly higher in patients who were >50 years of age (P<0.05). These data indicate that a number of these miRNAs may be important in the development of gastric cancer. In particular, miR-551b-3p, miR-133b, miR-100-5p and miR-363-3p may act as tumor suppressors in the development of gastric cancer. By contrast, miR-215 appears to exhibit oncogenic properties and promote the development of gastric cancer. In addition, the abnormal expression of miR-200a-3p may be associated with gender, while the abnormal expression of miR-429 may be associated with age in patients with gastric cancer. However, additional studies are required to delineate the underlying mechanisms of the association, and to explore their potential as valid biomarkers in the diagnosis, classification and prognosis of gastric cancer.

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Section: Discussionmentioning

confidence: 99%

Identification and characterization of tumor suppressor and oncogenic miRNAs in gastric cancer

Chen

Liu

et al. 2015

Oncology Letters

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“…Abnormal expression of miRNAs has been reported in a number of cervical cancer tissues and cell lines (16). These miRNAs are able to promote cell proliferation and reduce apoptosis, affect cell invasion, and eventually contribute to the initiation and progression of cervical cancer (1). miRNAs are very stable and permit easy detection of specific types of cancer (17).…”

Section: Discussionmentioning

confidence: 99%

Profiling analysis of circulating microRNA expression in cervical cancer

Nagamitsu

Nishi

Sasaki

et al. 2016

Molecular and Clinical Oncology

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Abstract. MicroRNA (miRNA) expression is altered in cancer cells and is associated with the development and progression of various types of cancer. Accordingly, miRNAs may serve as diagnostic or prognostic biomarkers in cancer patients. In this study, we attempted to analyze circulating exosomal miRNA in patients with cervical cancer. Total RNA was extracted from the serum of healthy subjects, subjects with cervical intraepithelial neoplasia (CIN) and patients with cervical cancer. We first investigated miRNA expression profiles in 6 serum samples from healthy subjects and patients with cervical cancer using the miRCURY LNA microRNA array. miRNAs with significant differences in expression were validated in a larger sample set by quantitative reverse transcription-polymerase chain reaction, using TaqMan gene expression assays. The results of the miRCURY LNA microRNA array indicated that 6 of 1,223 miRNAs found in serum samples from cervical cancer patients and normal controls exhibited a >3.0-fold change in expression level in subjects with cervical cancer, with a P-value of <0.01. In a validation set (n=131) that investigated the expression of 4 of the 6 miRNAs (miR-483-5p, miR-1246, miR-1275 and miR-1290), miR-1290 was found to have significantly higher expression levels in cervical cancer samples (n=45) compared with control samples (n=31). We also found that the median levels of these miRNAs were significantly higher in subjects with cervical cancer (n=45) compared with those in subjects with CIN (n=55). Circulating miRNAs were not correlated with clinicopathological parameters. However, receiver operating characteristic curve analysis suggested that these serum miRNAs may be useful diagnostic markers in cervical cancer. The expression of circulating miR-1290 was significantly higher in the blood of cervical cancer patients compared with that in controls and may thus serve as a useful biomarker in cervical cancer diagnosis. However, larger studies are required to fully elucidate the role of circulating exosomal miRNAs in cervical cancer.

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“…Collectively, these data suggest that the elevated levels of miR-186 and miR-150 in ectocervical cancerous tissue and cell lines may induce a downregulation of P2RX7 and thereby an inhibition of apoptosis. Along these lines, miR-886-5p was also demonstrated to inhibit apoptosis and be over-expressed in cervical cancer tissue (Li et al 2011a(Li et al , 2011b. Forced expression of miR-886-5p in the cervical cancer cell line H8 was associated with reduced apoptosis and increased cell proliferation.…”

Section: Cervixmentioning

confidence: 95%

“…While miR-21 appears to be upregulated in cervical cancers, miR-100 (Li et al 2011a(Li et al , 2011b and miR-372 (Tian et al 2011) expression is reduced. miR-100 expression was examined in normal cervical epithelium, cervical intraepithelial neoplasia, and cervical cancer in addition to five cervical cell lines (Li et al 2011a(Li et al , 2011b. qRT-PCR analysis revealed that miR-100 was highest in normal cervical epithelium and decrease with increasing stage of cancer.…”

Section: Cervixmentioning

confidence: 99%

The role of micro-RNAs in the female reproductive tract

Nothnick

2012

Reproduction

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Proper development and function of the female reproductive tract are essential for successful reproduction. Regulation of the differentiated functions of the organs that make up the female reproductive tract is well established to occur at multiple levels including transcription, translation, and posttranslational modifications. Micro-RNA (miRNA)-mediated posttranscriptional gene regulation has emerged as a fundamental mechanism controlling normal tissue development and function. Emerging evidence indicates that miRNAs are expressed within the organs of the female reproductive tract where they function to regulate cellular pathways necessary for proper function of these organs. In this review, the functional significance of miRNAs in the development and function of the organs of the female reproductive tract is discussed. Initial discussion focuses on the role of miRNAs in the development of the organs of the female reproductive tract highlighting recent studies that clearly demonstrate that mice with disrupted Dicer1 expression are sterile, fail to develop uterine glands, and have muted estrogen responsiveness. Next, emphasis moves to discussion on our current knowledge on the characterization of miRNA expression in each of the organs of the female reproductive tract. When possible, information is presented and discussed with respect to regulation, function, and/or functional targets of these miRNA within each specific organ of the female reproductive tract.

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Reduced miR-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein

Cited by 93 publications

References 39 publications

Identification and characterization of tumor suppressor and oncogenic miRNAs in gastric cancer

Identification and characterization of tumor suppressor and oncogenic miRNAs in gastric cancer

Profiling analysis of circulating microRNA expression in cervical cancer

The role of micro-RNAs in the female reproductive tract

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