Reduced Levels of Circulating Endothelial Cells and Endothelial Progenitor Cells in Patients with Heart Failure with Reduced Ejection Fraction

Lopes, José Fortes; Teixeira, Manuel; Cavalcante, Suiane; Gouveia, Marisol; Duarte, Ana Joana; Ferreira, Miriam; Simões, Marco; Conceição, Maria; Ribeiro, Ilda Patrícia; Gonçalves, Ana Cristina; Schmidt, Cristine; Jesus, Bruno Bernardes de; Almeida, Ramiro D.; Viamonte, Sofia; Santos, Mário; Ribeiro, Fernando

doi:10.1016/j.arcmed.2022.02.001

Cited by 8 publications

(9 citation statements)

References 32 publications

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“…The flow cytometry analysis (FACS-Calibur flow cytometer, Becton Dickinson, San Jose, CA, USA) was performed two to three days after the blood collection. Staining and analysis were performed using a protocol adapted from Ahmed, et al [ 35 ], as previously reported [ 11 ]. For the quantitative assessment of circulating EPCs and CECs by flow cytometry, whole blood samples were incubated for 10 min with FcR-blocking reagent to block unwanted binding of antibodies to human Fc receptor-expressing cells.…”

Section: Methodsmentioning

confidence: 99%

“…The number of EPCs is positively related to endothelial function [ 10 ] and has been used for the assessment of endothelial dysfunction [ 4 ]. Functionality and levels of EPCs are reduced in HF patients [ 11 , 12 ] and tend to decline with aging [ 13 ]. Indeed, reduced circulating levels of EPCs are related to increasing hazard ratios for all-cause mortality and cardiovascular death, independently of N-terminal pro B-type natriuretic peptide (NT-proBNP) levels [ 14 ], and circulating levels of EPCs are considered a strong and independent predictor of mortality in HF [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Maximal Exercise Improves the Levels of Endothelial Progenitor Cells in Heart Failure Patients

Cavalcante

Viamonte

Cadilha

et al. 2023

CIMB

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The impact of exercise on the levels of endothelial progenitor cells (EPCs), a marker of endothelial repair and angiogenesis, and circulating endothelial cells (CECs), an indicator of endothelial damage, in heart failure patients is largely unknown. This study aims to evaluate the effects of a single exercise bout on the circulating levels of EPCs and CECs in heart failure patients. Thirteen patients with heart failure underwent a symptom-limited maximal cardiopulmonary exercise test to assess exercise capacity. Before and after exercise testing, blood samples were collected to quantify EPCs and CECs by flow cytometry. The circulating levels of both cells were also compared to the resting levels of 13 volunteers (age-matched group). The maximal exercise bout increased the levels of EPCs by 0.5% [95% Confidence Interval, 0.07 to 0.93%], from 4.2 × 10−3 ± 1.5 × 10−3% to 4.7 × 10−3 ± 1.8 × 10−3% (p = 0.02). No changes were observed in the levels of CECs. At baseline, HF patients presented reduced levels of EPCs compared to the age-matched group (p = 0.03), but the exercise bout enhanced circulating EPCs to a level comparable to the age-matched group (4.7 × 10−3 ± 1.8 × 10−3% vs. 5.4 × 10−3 ± 1.7 × 10−3%, respectively, p = 0.14). An acute bout of exercise improves the potential of endothelial repair and angiogenesis capacity by increasing the circulating levels of EPCs in patients with heart failure.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Maximal Exercise Improves the Levels of Endothelial Progenitor Cells in Heart Failure Patients

Cavalcante

Viamonte

Cadilha

et al. 2023

CIMB

Self Cite

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“…A possible explanation could be the already established extended vascular inflammation in HF, no matter of the myocardium’s function, and the increased number of inflammatory factors. EPCs are also shown to be significantly lower in HF compared to subjects without HF but with other cardiovascular risk factors, as well as to healthy subjects without cardiovascular risk factors [ 21 , 70 ]. The extended vascular inflammation in HF and the atherosclerotic process that leads to suppressed response in mobilization of progenitor cells from the bone marrow could explain these findings.…”

Section: Circulating Endothelial and Progenitor Cellsmentioning

confidence: 99%

“…They either transform in mature circulating endothelial cells (CECs) or remain as precursor cells restoring the dysfunctional and injured endothelium and promoting vasculogenesis and angiogenesis [ 19 , 20 ]. Recent studies have shown that circulating levels of EPCs and CECs are significantly decreased in HF patients with vascular endothelial dysfunction and inflammation compared to age-matched healthy subjects without established cardiovascular disease [ 21 ]. Thus, CECs and EPCs could be suggested as potential biomarkers of the cellular response to vascular injury in patients with HF [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have shown that circulating levels of EPCs and CECs are significantly decreased in HF patients with vascular endothelial dysfunction and inflammation compared to age-matched healthy subjects without established cardiovascular disease [ 21 ]. Thus, CECs and EPCs could be suggested as potential biomarkers of the cellular response to vascular injury in patients with HF [ 21 ]. Although exercise has been suggested to have a positive impact on the mobilization of EPCs and the increase of CECs in cardiovascular diseases (especially HF), our knowledge still remains limited regarding this field.…”

Section: Introductionmentioning

confidence: 99%

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Exercise Training Effects on Circulating Endothelial and Progenitor Cells in Heart Failure

Kourek

Briasoulis

Zouganeli

et al. 2022

JCDD

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Heart failure (HF) is a major public health issue worldwide with increased prevalence and a high number of hospitalizations. Patients with chronic HF and either reduced ejection fraction (HFrEF) or mildly reduced ejection fraction (HFmrEF) present vascular endothelial dysfunction and significantly decreased circulating levels of endothelial progenitor cells (EPCs). EPCs are bone marrow-derived cells involved in endothelium regeneration, homeostasis, and neovascularization. One of the unsolved issues in the field of EPCs is the lack of an established method of identification. The most widely approved method is the use of monoclonal antibodies and fluorescence-activated cell sorting (FACS) analysis via flow cytometry. The most frequently used markers are CD34, VEGFR-2, CD45, CD31, CD144, and CD146. Exercise training has demonstrated beneficial effects on EPCs by increasing their number in peripheral circulation and improving their functional capacities in patients with HFrEF or HFmrEF. There are two potential mechanisms of EPCs mobilization: shear stress and the hypoxic/ischemic stimulus. The combination of both leads to the release of EPCs in circulation promoting their repairment properties on the vascular endothelium barrier. EPCs are important therapeutic targets and one of the most promising fields in heart failure and, therefore, individualized exercise training programs should be developed in rehabilitation centers.

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Reaktion der endothelialen Progenitorzellen auf ein Multikomponenten-Trainingsprogramm bei Erwachsenen mit kardiovaskulären Risikofaktoren

Cavalcante

Teixeira

Gouveia

et al. 2023

Ger J Exerc Sport Res

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Reduced Levels of Circulating Endothelial Cells and Endothelial Progenitor Cells in Patients with Heart Failure with Reduced Ejection Fraction

Cited by 8 publications

References 32 publications

Maximal Exercise Improves the Levels of Endothelial Progenitor Cells in Heart Failure Patients

Maximal Exercise Improves the Levels of Endothelial Progenitor Cells in Heart Failure Patients

Exercise Training Effects on Circulating Endothelial and Progenitor Cells in Heart Failure

Reaktion der endothelialen Progenitorzellen auf ein Multikomponenten-Trainingsprogramm bei Erwachsenen mit kardiovaskulären Risikofaktoren

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