Reduced Immune Phagocytosis of Monocytes from Neonates Whose Mothers Produce HLA Antibodies

Neppert, J.; Mueller‐Eckhardt, G.; Heine, O.

doi:10.1111/j.1423-0410.1988.tb03896.x

Cited by 17 publications

(20 citation statements)

References 12 publications

(5 reference statements)

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“…For the most part, our data agree with other reports [3][4][5][6][7]. In some of these previous studies (table 2), the higher rates of alloimmunization may have been due to the emergence of antibody during the period post partum, the inclusion of large numbers of multipa rous women and to different techniques of antibody detection.…”

Section: Discussionsupporting

confidence: 81%

“…A high frequency of alloimmunization against leu kocyte antigens has been clearly established in women with a history of pregnancy [3][4][5][6][7], Although this suggests that blood products obtained from pa rous women, in general, may increase the risks of transfusion due to passive antibody administration, many of these earlier studies were retrospective, and little regard was paid to the other factors that deter mine eligibility to donate blood. Thus, the genuine risks posed by a biological mother who wishes to do nate blood products for her neonate shortly after de livery are unknown.…”

Section: Introductionmentioning

confidence: 99%

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Biological Mothers May Be Dangerous Blood Donors for Their Neonates

Elbert

Strauss²,

Barrett³

et al. 1991

Acta Haematol

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Premature neonates require blood transfusions, and biological parents may wish to be directed donors. Biological mothers pose a potential danger because their plasma may contain antibodies that will react with blood cell antigens inherited by the infant from the father. We studied 25 healthy, pregnant women at the time of delivery for the presence of antibodies against red blood cell, leukocyte and platelet antigens. Mothers known to have red cell antibodies earlier in pregnancy were excluded, and no new red cell antibodies appeared at delivery. Antileukocyte and antiplatelet antibodies were found in 16 and 12% of mothers, respectively. Because these antibodies have the potential to cause adverse reactions when transfused passively, we suggest that either biological mothers not provide blood components containing plasma for their neonates or that maternal red cells and platelets be given as washed products.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Biological Mothers May Be Dangerous Blood Donors for Their Neonates

Elbert

Strauss²,

Barrett³

et al. 1991

Acta Haematol

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“…This was based on the observation that HLA class I antibodies are more common than HLA class II anti bodies in pregnant women, and the demonstration that the FcyRI-dependent function of monocytes from newborn ba bies whose mothers produce HLA class I and/or class II an tibodies was significantly inhibited [5].…”

Section: Discussionmentioning

confidence: 99%

“…The presence of FcyRI-blocking antibodies is indicated by a reduction in the level of erythrophagocytosis or red cell lysis. Although both anti-HLA class I and class II antibodies inhibit lysis and phagocytosis in vitro [1,4], data regarding the specificity of antibodies which may confer in vivo protection are conflicting [1,5,6].…”

mentioning

confidence: 99%

Inhibition of the Monocyte Chemiluminescent Response to Anti-D- Sensitized Red Cells by FcγRI-Blocking Antibodies Which Ameliorate the Severity of Haemolytic Disease of the Newborn

Shepard¹,

Noble²,

Filbey³

et al. 1996

Vox Sanguinis

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Luminol-enhanced chemiluminescence (CL) may be used to measure the metabolic response of human monocytes to red cells sensitized by anti-D. The functional activity of maternal anti-D when measured in this way correlates with the severity of haemolytic disease of the newborn (HDN) in D-positive fetuses. Occasionally, however, women with levels of functionally active anti-D predictive of moderate to severe HDN may deliver D-positive babies with unexpectedly mild disease. In the current study we have shown that serum from 12 of 15 such women contained monocyte-binding IgG antibodies which blocked FcγRI and inhibited the CL response of moncytes to red cells sensitized with monoclonal anti-D. In contrast, FcγRl-blocking antibodies were found in the serum from only 4 of 11 women who were matched for anti-D activity but who delivered babies with severe HDN (p<0.05). Antibodies responsible for inhibition of CL responses were predominantly against HLA class I antigens. The CL response of monocytes to sensitized red cells was at least as sensitive to inhibition by FcγRl- blocking antibodies as were phagocytic and lytic responses. Our data suggest that inhibition of the CL test is an objective, sensitive and relatively simple technique for detecting and investigating FcγRl-blocking antibodies which may ameliorate the severity of HDN.

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“…In contrast, a serum sample obtained from the newborn on the first day after birth was not able to inhibit the immune phagocytosis of either the paternal or blood do nor monocytes which had previously given positive IPI test results with the gravida's serum. Table 3 contains the results of the IPI test performed with absorbed and non-absorbed maternal serum and blood donor monocytes with a various ition [17]. Dooren et al [5] could show that 7 out of 9 sera obtained from women with unexpectedly mild HDN had a blocking effect on the function of monocytes from their partners after the absorption of HLA class I antibodies.…”

Section: Methodsmentioning

confidence: 99%

Mild Course of Fetal Rh D Haemolytic Disease due to Maternal Alloimmunisation to Paternal HLA Class I and II Antigens

Eichler¹,

Zieger²,

Nepper³

et al. 1995

Vox Sanguinis

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The patient is a pregnant women of African origin with a prior history of spontaneous abortion and newborn dystrophy. The investigation showed an anti-Rh D antibody (IgG isotypes 1 and 3) with an indirect antiglobulin tube test (IAT) titre of 1:512. The monocyte monolayer assay (MMA) proved clearly the interaction of Fc receptors with the maternal anti-D, and so a clinical significance was expected. In spite of this, no signs of severe haemolysis in the Rh-D-positive and direct antiglobulin test-positive fetus could be observed. Furthermore, two HLA class I and II alloantibodies (anti-A10, anti-DR13) directed against paternal and fetal antigens were detected in the serum of the gravida. Both antibodies showed an inhibitory effect on the in vitro phagocytosis capacity of mononuclear cells expressing at least one of the corresponding HLA antigens (immunophagocytosis inhibition (IPI) test). Thus, the mild course of haemolytic disease may be explained by an effective inhibition of the fetal mononuclear phagocyte system by maternal HLA class I and/or class II antibodies resulting in a diminished destruction of anti-D-coated fetal red blood cells.

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Reduced Immune Phagocytosis of Monocytes from Neonates Whose Mothers Produce HLA Antibodies

Cited by 17 publications

References 12 publications

Biological Mothers May Be Dangerous Blood Donors for Their Neonates

Biological Mothers May Be Dangerous Blood Donors for Their Neonates

Inhibition of the Monocyte Chemiluminescent Response to Anti-D- Sensitized Red Cells by FcγRI-Blocking Antibodies Which Ameliorate the Severity of Haemolytic Disease of the Newborn

Mild Course of Fetal Rh D Haemolytic Disease due to Maternal Alloimmunisation to Paternal HLA Class I and II Antigens

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