Reduced Glutathione Enhances Fertility of Frozen/Thawed C57BL/6 Mouse Sperm after Exposure to Methyl-Beta-Cyclodextrin1

Tanaka, Takeo; Nakagata, Naomi

doi:10.1095/biolreprod.111.092536

“…In addition, the frozen-thawed sperm which was preincubated with �� 3) . Finally, reduced glutathione supported sperm penetration through zona pellucida (ZP) by changing and loosening the reticulate structure of ZP 4) . Combined with these compounds for the protocol of sperm cryopreservation and IVF, we established a reliable system for producing mouse embryo via IVF using cryopreserved sperm.…”

Section: Toru Takeomentioning

confidence: 99%

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2012

Exp. Anim.

0

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�� EMBO J. in 1988. Then, I generated Tg mice carrying the tax gene of HTLV-I, which is the causative agent of adult T cell leukemia. Because these transgenic mice spontaneously developed autoimmune arthritis resembling rheumatoid arthritis in humans, I suggested that HTLV-I is one of etiological agents of rheumatoid arthritis (Science, 1991). Through the analysis of these Tg mice, I found that Tax-induced IL-1 might play a critical role in the pathogenesis of arthritis. Then, we knocked out all �� in the febrile response and glucocorticoid induction under stress (J. Exp. Med., 1988). Furthermore, we found that IL-1Ra-��J. Exp. Med., 2000; �� TNF is a good candidate for the treatment of these diseases (J. Clin. Invest., �� because this gene expression was enhanced in both HTLV-I Tg and IL-1Ra KO mice. We showed that the development of arthritis was strongly suppressed in IL-17 KO mice, suggesting that IL-17 plays a critical role in the development of arthritis (Immunity, 2002; PNAS, 2003). Furthermore, we showed that IL-17F, the molecule with the highest homology with IL-17A in the same family, is important for the host defense against microbial mucoepithelial infection (Immunity, 2009; Immunity, 2011). To further analyze the mechanisms of the development of autoimmunity and arthritis, we knocked out genes which were highly expressed in arthritic joints. Among them, we showed that Dectin-1 and Dectin-2, C-type lectin receptors, is the receptor for ��Nat. Immunol., 2007). Interestingly, Th17 cell differentiation was preferentially induced by these signals, suggesting that fungal infection may cause autoimmunity (Immunity, 2010). We also showed that KO mice of Dcir, another C-type lectin family member, develop autoimmune enthesitis and sialadenitis (Nat. Med., 2008). These results indicate that non-TLR innate immune molecules such as C-type lectins are important for the regulation of the acquired immune system and can be a good target for the treatment of autoimmune diseases. Taken together, I could show through these studies that gene manipulated mice are useful for the development of disease models and analysis of the pathogenic mechanisms.

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“…Glutathione could protect sperm due to its strong antioxidant activities by inhibiting the lipid peroxidation of red deer sperm during freezing-thawing processes. Glutahione was reported to be used as a cryoprotectant for the sperm of mouse, canine, boar, ovine, bull and red deer (Sławeta & Laskowska, 1987, Takeo & Nakagata 2011, Perez et al, 2012, Anel-López et al, 2015and Lucio et al, 2016. Recently, Wang and Dong (2017) reported that glutathione addition to the extender improved sperm quality, including sperm motility, acrosomal integrity, plasma-membrane integrity and sperm morphology.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

Shamiah¹,

El-Karim

²

,

Eshera³

2017

Journal of Sustainable Agricultural Sciences

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IntroductionThe increasing use of artificial insemination (AI) in poultry industry emphasizes the need for good quality sperm distribution. A proper evaluation of semen prior to AI or storage is very important to avoid losses in fertility. Because semen evaluation is extremely important for semen storage and AI programs, a rapid, economical, objective and fertility predictive method of semen evaluation would be beneficial for the poultry industry protocols. Determination of antioxidant enzymes concentration in semen has been practiced for a long time (Peters, 2008).Lipids are a basic component of semen, contributing to the membrane structure of spermatozoa, the metabolism of the sperm cells, and their ability to capacitate and fertilize the female gamete. In birds, the lipid composition of spermatozoa has an influence on fertility (Ansah and Buckland, 1982). El-Saadany (2002) reported that increasing storage time of diluted semen from local chicken breed for 0, 24, 48 and 72 hr decreased significantly (P<0.01) the fertility rate (FR) by 5.12, 9.55 and 12.53%, respectively. Shamiah et al. (2017) reported that GSH supplementation in semen extender significantly (P<0.05) increased FR. Semen supplemented with 0.2 mM GSH (74.5%) showed higher FR than those supplemented with 0.6 mM GSH or free semen (59.9 and 59.2%, respectively).Sperm cells have a high content of unsaturated T HIS STUDY was carried out to evaluate the effect of glutathione (GSH) supplementation at levels of 0.0 (control), 0.2, 0.4 and 0.6 mM to diluted semen stored for 0, 24, 48 and 72 hr at 5°C, on antioxidant enzymes activities in seminal plasma, semen characteristics and fertility rate (FR) of cocks Inshas strain. Supplementation of 0.6 mM GSH to the diluted cocks semen significantly increased (P<0.05) the concentration of alkaline phosphatase (ALP) (40.2 u/ml) compared to other treatments (33.0, 27.8 and 28.3 u/ml). Superoxide dismutase (SOD) concentration in seminal plasma was not significantly affected (P<0.05) by different levels of GSH, storage period or interaction between levels of GSH and storage period. Glutathione peroxidase (GSH-Px) concentration in seminal plasma was not affected by different levels of GSH (P<0.05). Total antioxidant capacity (TACO) in seminal plasma was significantly affected (P<0.05) by different levels of GSH, storage period or interaction between them. There was a positive correlation (r=0.089 and r = 0.168) between SOD concentration and (GSH-Px and TAOC) concentration, but negative correlation (r=-0.119) with ALP concentration. Positive insignificant correlation between (ALP, SOD and TACO) concentration in seminal plasma with the progressive motility, live sperm, curled tail and fertility, while positive significant correlation between GSH-Px concentration with progressive motility, curled tail and fertility. It could be concluded that the addition of GSH to semen extender of cockerels especially at levels 0.2 mM improved the antioxidant enzymes activities, which play a vital role in lowering the deleterious effe...

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“…In recent years the cryopreservation of mouse spermatozoa was improved to become a reliable tool [4][5][6]. However, an in vitro-fertilization required to recover a line from frozen spermatozoa is complex [7,8] the success is often influenced externally, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…genetic background, mutation, osmotic stress, batches of the culture media used, environment, etc. [4][5][6][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Age-related Yields of Mouse Sperm and Oocytes

Ramin¹,

Letz²,

Schwab³

et al. 2017

J Anim Res Nutr

2

0

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Title: Genetically modified animals, in particular mice, are unique mutants with a major scientific potential. Cryopreservation of pre-implantation embryos or of spermatozoa is a common approach to protect those lines against loss or to archive them. Prerequisite to take a mutant line out of a breeding nucleus is the cryopreservation of a sufficient number of samples and a powerful quality assessment.Background: Several common protocols to cryopreserve spermatozoa and subsequently to forward these to an in vitro-fertilization under standardized conditions are published using donors of a strictly limited age-span. In practice, several limitations happen frequently for those approaches, e.g. the availability of genetically modified males, in particular if a line is in an experimental use or the mutation leads to restrictions. Female donors are often received from external sources and are subsequently not available before weaning. They are subject of several delays to induce an early superovulation. The aim of this report is to study the suitable age span of sperm and oocyte donors.

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Reduced Glutathione Enhances Fertility of Frozen/Thawed C57BL/6 Mouse Sperm after Exposure to Methyl-Beta-Cyclodextrin1

Cited by 159 publications

References 41 publications

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Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

Age-related Yields of Mouse Sperm and Oocytes

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