Reduced glutamate binding in rat dorsal vagal complex after nodose ganglionectomy

Lewis, Stephen J.; Verberne, Anthony J.M.; Summers, Roger J.; Beart, Philip M; Cincotta, Marion

doi:10.1016/0361-9230(88)90026-3

Cited by 40 publications

(13 citation statements)

References 13 publications

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“…We have previously used this technique to demonstrate the presynaptic location of adenosine A2a receptors (Castillo-Melendez et al, 1994); angiotensin II receptors (Lewis et al, 1986) and vasopressin V1 receptors (Gao et al, 1992) in the rat NTS. In addition, nodose ganglionectomy has also been employed to study the cellular localization in the NTS of glutamate receptors (Lewis et al, 1988), neurotensin receptors (Kessler & Beaudet, 1989), cholecystokinin receptors (Ladenheim et al, 1988), muscarinic cholinoceptor and delta-opioid receptors (Leslie et al, 1989), 5-HT3 receptors (Merahi et al, 1992) and substance P receptors (Manaker & Zucchi, 1993).…”

Section: Discussionmentioning

confidence: 99%

Functional dopamine D₂ receptors on rat vagal afferent neurones

Lawrence

Krstew

Jarrott

1995

British J Pharmacology

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1 In the present study in vitro electrophysiology and receptor autoradiography were used to determine whether rat vagal afferent neurones possess dopamine D2 receptors. 2 Dopamine (10-300IM) elicited a temperature-and concentration-dependent depolarization of the rat isolated nodose ganglion preparation. When applied to the tissue 15 min prior to agonist, raclopride (10 M), clozapine (10I1M) or a mixture of raclopride and clozapine (1O gM each) all produced a threefold parallel shift to the right of the dopamine concentration-response curve. In contrast, SCH 23390 (100 nM), phentolamine and propranolol (1 jAM each) failed to antagonize the dopamine-mediated depolarization.3 [125I]-NCQ 298 (0.5 nM), a D2 selective radioligand, bound topographically to sections of rat brainstem. Densitometric quantification of autoradiograms revealed 93.8 ± 0.5% specific binding of this salicylamide radioligand, as determined by raclopride (1O iM, n = 10 animals). Binding was highest in the nucleus tractus solitarius (NTS), particularly the medial and gelatinous subnuclei. In addition, specific binding was also observed in the interpolar spinal trigeminal nucleus and the inferior olive. 4 Unilateral nodose ganglionectomy caused a 36.6 ± 3.0% reduction in specific binding in the denervated NTS compared to the contralateral NTS. Furthermore, the loss of binding was confined to the dorsal aspect of the medial subnucleus of the NTS. Sham surgery had no effect on the binding of ['25I]-NCQ 298 in rat brainstem. 5 The present data provide evidence for the presence of functionally relevant dopamine D2 receptors on both the soma and central terminals of rat vagal afferent neurones. In addition, the majority of D2 receptors in the rat NTS appear to be located postsynaptically with respect to vagal terminals, and are presumably located either on ascending glossopharyngeal terminals, descending terminals from higher brain regions or on neuronal cell bodies within the NTS.

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Section: Discussionmentioning

confidence: 99%

Functional dopamine D₂ receptors on rat vagal afferent neurones

Lawrence

Krstew

Jarrott

1995

British J Pharmacology

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“…Considering the presence of NR1 mRNA in rat nodose ganglia (Shigemoto et al, 1992), at least some of the NR1 immunoreactivity in the NTS may originate in the vagal ganglia. Furthermore, ligation of afferent fibers passing from the nodose ganglion toward their termination in the NTS has lead to a reduction in number of receptors in the NTS (Lewis et al, 1988). Such a reduction could indicate that the receptors reside on presynaptic membranes after transport from the perikaryon in the nodose ganglion, where they are synthesized (Lewis et al, 1987;Cincotta et al, 1989).…”

Section: Distribution In the Nucleus Of The Tractus Solitariusmentioning

confidence: 98%

Glutamate receptor subunits in the nucleus of the tractus solitarius and other regions of the medulla oblongata in the cat

et al. 1998

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The nucleus of the tractus solitarius (NTS) is a primary termination zone for laryngeal, gustatory, cardiovascular, respiratory, gastrointestinal, and other visceral afferents. Although considerable information is available on the neurochemical aspects of the NTS in general, very little is known about glutamate receptors that may underlie many of the different functions mediated by the NTS. In addition, most previous glutamate receptor distribution studies were performed in the rat, whereas the cat, the subject of many physiological experiments involving the NTS, has received little attention. In the present study, the immunohistochemical distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-selective glutamate receptor subunits (GluR1, GluR2/3, GluR4) and the N-methyl-D-aspartate (NMDA) receptor subunit NR1 in the cat caudal brainstem was investigated by using subunit-specific antibodies. In the NTS, statistically significant differences were seen in the distribution of each antibody. Highest labeling was seen for GluR2/3 in most subnuclei, whereas GluR1-immunoreactive neurons were found more frequently than were NR1- or GluR4-immunoreactive neurons. GluR1 immunolabeling was particularly high in the interstitial subnucleus, whereas GluR2/3 immunolabeling was particularly high in the intermediate subnucleus. Qualitatively, labeling for GluR4 was most common in glia. The present results indicate that glutamate receptors show different subunit distributions in the subnuclei of the NTS and in other adjacent structures. This finding suggests that neurons in these structures are designed to respond differently to excitatory input.

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“…Glutamate may be effective in the dorsal vagal complex and involved in the vagal reflex [1][2][3][4]. Vagovagal reflex plays an integral role in the regulation of gastrointestinal motor functions [5][6].…”

mentioning

confidence: 99%

The effect of N-methyl-d-aspartate receptor antagonist (memantine) on esophageal and gastric smooth muscle: functional investigation in a rat hydrocephalus model

Bektaş

Tugay

et al. 2008

Journal of Pediatric Surgery

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Reduced glutamate binding in rat dorsal vagal complex after nodose ganglionectomy

Cited by 40 publications

References 13 publications

Functional dopamine D₂ receptors on rat vagal afferent neurones

Functional dopamine D₂ receptors on rat vagal afferent neurones

Glutamate receptor subunits in the nucleus of the tractus solitarius and other regions of the medulla oblongata in the cat

The effect of N-methyl-d-aspartate receptor antagonist (memantine) on esophageal and gastric smooth muscle: functional investigation in a rat hydrocephalus model

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Reduced glutamate binding in rat dorsal vagal complex after nodose ganglionectomy

Cited by 40 publications

References 13 publications

Functional dopamine D2 receptors on rat vagal afferent neurones

Functional dopamine D2 receptors on rat vagal afferent neurones

Glutamate receptor subunits in the nucleus of the tractus solitarius and other regions of the medulla oblongata in the cat

The effect of N-methyl-d-aspartate receptor antagonist (memantine) on esophageal and gastric smooth muscle: functional investigation in a rat hydrocephalus model

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Functional dopamine D₂ receptors on rat vagal afferent neurones

Functional dopamine D₂ receptors on rat vagal afferent neurones