Reduced Expression of Early Growth Response-1 Gene in Leiomyoma as Identified by mRNA Differential Display

Pambuccian, Corina; Oprea, Gabriela; Lakatua, D

doi:10.1006/gyno.2001.6549

“…In contrast, prostatic cancer (28) , gastric cancer (29) , and nasopharyngeal carcinoma cell lines (30) overexpress this gene. In our study, the EGR-1 gene expression is underexpressed consistently in all myoma tissues (Table 3), which supports the previous results (31) . We consider that EGR-1 could represent an important event in leiomygenesis, but the exact regulation mechanism of this gene in uterine leiomyoma needs further investigation.…”

Section: Discussionsupporting

confidence: 95%

“…Uterine leiomyomas are the most common pelvic tumors in women and are associated with various clinical problems including abnormal uterine bleeding, pelvic pain, urinary frequency, constipation, impaired fertility, and spontaneous abortion. The incidence rate of diagnosed uterine leiomyoma was 12.8 per 1000/year women in one prospective cohort of women aged [25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] (1) , and leiomyoma are the most commonly listed discharge diagnoses for hysterectomy in the United States (2) . Despite the clinical and public health importance of uterine leiomyoma, no certain drug treatment except surgery and no prevention method give us significant limitations for the management of these tumors.…”

mentioning

confidence: 99%

Profiling of differentially expressed genes in human uterine leiomyomas

Lee¹,

Lee²,

Rho³

et al. 2005

Int J Gynecol Cancer

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Uterine leiomyomas are very common benign tumors resulting in clinically serious gynecological problems in women of reproductive age. Approximately, 1% of leiomyosarcoma was reported to arise in a preexisting leiomyoma. However, the molecular basis of these tumors is poorly understood. To understand the molecular changes during leiomyoma development, we profiled differentially expressed genes in ten paired leiomyoma and normal myometrial tissues using cDNA microarray chip analysis. We identified 67 genes (27 overexpressed and 40 underexpressed) which were scored as differentially expressed at least twofold in at least eight of ten patients. Eighteen of 67 genes have been already reported to be differentially expressed without their established functions in uterine leiomyoma and others have never been reported. Subsequently, the relative expression levels of representative genes from identified 67 genes were confirmed by reverse-transcriptase polymerase chain reaction and immunohistochemistry and were found to be consistent with the microarray data. This study could provide a new insight into the understanding of leiomyoma and leiomyosarcoma.

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“…The molecular causes of the disease remain unknown [1][2][3][4][5][11][12][13][14] . A variety of techniques, including differential hybridization, differential display PCR, representational difference analysis, suppression subtractive hybridization (SSH), and gene array, have been used to identify the genes whose expression is selectively altered in uterine leiomyoma [1][2][3][12][13][14] . All these methods are used to analyze the mRNA expression levels of uterine leiomyoma relative genes.…”

Section: Discussionmentioning

confidence: 99%

“…Uterine leiomyomas are the most common tumor in the female genital tract, but their etiology and the molecular basis of their development and growth are unclear [1][2][3][4][5] . So it is very important to explore the molecular mechanism of the cause of uterine leiomyoma at protein level by studying the differential proteins between uterine leiomyoma and normal myometrium.…”

mentioning

confidence: 99%

Identification of differential proteins in uterine leiomyoma by two-dimensional electrophoresis

Zhu

¹

,

Zhu

²

,

Lu

³

et al. 2006

Chin. J. Cancer Res.

2

0

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Objective: To establish and optimize the two-demensional electrophoresis maps of uterine leiomyoma and to study the difference of global protein patterns between uterine leiomyoma and normal myometrium. Methods: Using Two-dimensional electrophoresis followed by computer-assisted image analysis, the differential proteins between uterine leiomyoma and normal myometrium were compared.Results: The well-resolved and reproducible two-dimensional gel electrophoresis patterns of uterine leiomyoma and normal myometrium were established. Totally 1085±108 and 1103±151 protein spots were obtained by using the pH 4-7 IPG strips in uterine leiomyoma and normal myometrium map, respectively, of which 7 spots increased and 15 spots decreased in quantity in uterine leiomyoma compared with normal myometrium. Conclusion: The differentially expressed proteins are useful for studying the mechanism of the cause of uterine leiomyoma.

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“…The incidence rate of diagnosed uterine leiomyoma was 12.8 per 1000/year women in one prospective cohort of women aged [25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] (1) , and leiomyoma are the most commonly listed discharge diagnoses for hysterectomy in the United States (2) . Despite the clinical and public health importance of uterine leiomyoma, no certain drug treatment except surgery and no prevention method give us significant limitations for the management of these tumors.…”

mentioning

confidence: 99%

Profiling of differentially expressed genes in human uterine leiomyomas

Lee¹,

Kong²,

Lee³

et al. 2005

Int J Gynecol Cancer

View full text Add to dashboard Cite

Uterine leiomyomas are very common benign tumors resulting in clinically serious gynecological problems in women of reproductive age. Approximately, 1% of leiomyosarcoma was reported to arise in a preexisting leiomyoma. However, the molecular basis of these tumors is poorly understood. To understand the molecular changes during leiomyoma development, we profiled differentially expressed genes in ten paired leiomyoma and normal myometrial tissues using cDNA microarray chip analysis. We identified 67 genes (27 overexpressed and 40 underexpressed) which were scored as differentially expressed at least twofold in at least eight of ten patients. Eighteen of 67 genes have been already reported to be differentially expressed without their established functions in uterine leiomyoma and others have never been reported. Subsequently, the relative expression levels of representative genes from identified 67 genes were confirmed by reverse-transcriptase polymerase chain reaction and immunohistochemistry and were found to be consistent with the microarray data. This study could provide a new insight into the understanding of leiomyoma and leiomyosarcoma.

show abstract

Reduced Expression of Early Growth Response-1 Gene in Leiomyoma as Identified by mRNA Differential Display

Cited by 12 publications

References 20 publications

Profiling of differentially expressed genes in human uterine leiomyomas

Profiling of differentially expressed genes in human uterine leiomyomas

Identification of differential proteins in uterine leiomyoma by two-dimensional electrophoresis

Profiling of differentially expressed genes in human uterine leiomyomas

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