Reduced expression of CRHR2 and Sp-1 in myocardium of ovariectomized rats is improved by exercise training

Abstract

Exercise training has been looked on as a non-pharmacologic approach to treating ovariectomy (OVX)-induced dysfunctions. In this study, we investigated whether chronic exercise impacts on expression of urocortins (UCNs) and corticotropin-releasing hormone receptor type 2 (CRHR2) in myocardium of OVX rats. Bilateral OVX or sham-operation was performed under anesthesia. Both groups were then divided into two subgroups, with or without treadmill training for 8 weeks. It was found that OVX as well as exercise did not affect the mRNA levels of UCN, UCN2 and UCN3 in myocardium. OVX caused down-regulation of CRHR2 in myocardium. Exercise training reversed the OVX-induced reduction of CRHR2, but had no influence on CRHR2 level in sham rats. OVX resulted in a decrease in estrogen receptor α (ERα) expression in myocardium, which was restored by exercise. Moreover, exercise training also reversed OVX-induced down-regulation of specific protein-1 (Sp-1) expression in myocardium. CRHR2 expression level correlated with Sp-1 and ERα level in myocardium. These results indicate that exercise training can restore the CRHR2 level in myocardium of OVX rats, which is associated with ERα and Sp-1 expression.

Metformin ameliorates body mass gain and early metabolic changes in ovariectomized rats

“…Natural menopause in rodents occurs with the arrest of regular ovarian cycling, followed by irregular cycling, and then an arrest in cycling that maintains a constant cycle phase without a reduction in the estradiol levels (45). We did not observe any significant changes in serum 17β-estradiol levels in the OVX rats at 21 days, although some previous studies have reported decreased serum estradiol in models of ovariectomy (7, 46). Vaginal smears showed that the OVX and OVX + M rats were in a constant diestrous phase (data not shown), and uterus mass was hypotrophic in these two groups, demonstrating a low estradiol effect.…”

“…In this study, we used ovariectomized animals, a model in which animals have both ovaries removed and therefore mimics the estrogen deficiency that occurs during menopause. Ovariectomy is frequently used in literature for a better understanding of metabolic disorders of menopause (6, 7), and for testing pharmacological alternatives to prevent menopause disturbances (8, 9).…”

Integrative Bioinformatics Analysis Reveals That Infarct-Mediated Overexpression of Potential miR-662/CREB1 Pathway-Induced Neuropeptide VIP Is Associated with the Risk of Atrial Fibrillation: A Correlation Analysis between Myocardial Electrophysiology and Neuroendocrine

“…Exercise is an important way to promote human health [ 56 , 57 ]. Exercise training was also found to improve the expression of SP1 and NFKB1 in the heart [ 58 , 59 ].…”