Reduced expression of claudin-7 correlates with invasion and metastasis in squamous cell carcinoma of the esophagus

Usami, Yu; Chiba, Hideki; Nakayama, Fumihito; Ueda, Junya; Matsuda, Yoshiko; Sawada, Norimasa; Komori, Takahide; Ito, Akihiko; Yokozaki, Hiroshi

doi:10.1016/j.humpath.2005.12.018

Cited by 136 publications

(131 citation statements)

References 24 publications

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“…Claudin genes, which code for tight junction proteins, are believed to play a role in cancer cell invasion and metastasis (21,22) and have been implicated in breast and ovarian cancer. Reduced expression of claudin 7 was associated with invasion and metastasis of esophageal cancers (54). Claudin 3 was recently shown to be down-regulated in invasive, compared with noninvasive, breast cancer cells (55).…”

Section: Discussionmentioning

confidence: 98%

Functional Characterization of a Novel BRCA1-Null Ovarian Cancer Cell Line in Response to Ionizing Radiation

DelloRusso

Welcsh

Wang

et al. 2007

Molecular Cancer Research

101

110

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The breast and ovarian cancer susceptibility gene BRCA1 plays a major role in the DNA damage response pathway. The lack of well-characterized human BRCA1-null cell lines has limited the investigation of BRCA1 function, particularly with regard to its role in ovarian cancer. We propagated a novel BRCA1-null human ovarian cancer cell line UWB1.289 from a tumor of papillary serous histology, the most common form of ovarian carcinoma. UWB1.289 carries a germline BRCA1 mutation within exon 11 and has a deletion of the wild-type allele. UWB1.289 is estrogen and progesterone receptor negative and has an acquired somatic mutation in p53, similar to the commonly used BRCA1-null breast cancer cell line HCC1937. We used ionizing radiation to induce DNA damage in both UWB1.289 and in a stable UWB1.289 line in which wild-type BRCA1 was restored. We examined several responses to DNA damage in these cell lines, including sensitivity to radiation, cell cycle checkpoint function, and changes in gene expression using microarray analysis. We observed that UWB1.289 is sensitive to ionizing radiation and lacks cell cycle checkpoint functions that are a normal part of the DNA damage response. Restoration of wild-type BRCA1 function in these cells partially restores DNA damage responses. Expression array analysis not only supports this partial functional correction but also reveals interesting new information regarding BRCA1-positive regulation of the expression of claudin 6 and other metastasis-associated genes and negative regulation of multiple IFN-inducible genes. (Mol Cancer Res 2007;5(1):35 -45)

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Section: Discussionmentioning

confidence: 98%

Functional Characterization of a Novel BRCA1-Null Ovarian Cancer Cell Line in Response to Ionizing Radiation

DelloRusso

Welcsh

Wang

et al. 2007

Molecular Cancer Research

101

110

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“…[13][14][15][16][17][19][20][21]27 Tight junctions' alterations have been observed in thyroid neoplasms by freeze-fracture analysis. 28,29 In these studies, aberrant tight junction formation characterized poorly differentiated carcinomas or compact oncocytic tumors.…”

Section: Discussionmentioning

confidence: 99%

“…Reduced claudins' expression and associated loss of functional tight junctions are usually a feature of poorly differentiated tumors of various origins (ie breast, esophageal, gastric, colon and thyroid carcinomas). [14][15][16]30,31 Apparently, loss of claudins expression results in loss of cell-to-cell adhesion, thereby preventing the cells to form organized epithelial structures and leading in an undifferentiated phenotype. Undifferentiated carcinomas of the thyroid gland are the result of anaplastic transformation of a pre-existing well-differentiated tumor.…”

Section: Discussionmentioning

confidence: 99%

“…Different claudins are found at the tight junction of diverse epithelial cells, 11 and this accounts for the observed differences in permeability and electrical resistance of various epithelia. 2,8,10 Previous studies have shown that occludin and claudin-1, -4 and -7 are deregulated in a variety of malignancies; for example, loss of occludin expression or upregulation of claudin-3 and -4 accompanies the progression of endometrial carcinoma, 12,13 reduced expression of claudin-1 correlates with poorer differentiation, disease recurrence and poor survival in stage II colonic cancer, 14 claudin-7 expression has been found reduced in mammary, 15 esophageal, 16 and head and neck carcinomas, 17 whereas claudin-4 overexpression decreases the invasiveness and metastatic potential of pancreatic cancer cells. 18 On the other hand, various cancers (ie ovarian, colon, cervical cancer) are associated with elevated tight junctions proteins expression levels or protein dislocation to the cytoplasm.…”

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confidence: 99%

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Tight junctions in thyroid carcinogenesis: diverse expression of claudin-1, claudin-4, claudin-7 and occludin in thyroid neoplasms

et al. 2008

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Claudins and occludin are integral constituents of tight junctions and are deregulated in a variety of malignancies. Their role in thyroid carcinogenesis has not yet been elucidated. This study investigates the expression of occludin and claudin-1, -4 and -7 in thyroid neoplasms. Ninety-one thyroid neoplasms (15 follicular adenomas, 15 follicular carcinomas, 26 papillary carcinomas, 16 papillary microcarcinomas, 8 medullary carcinomas, 3 poorly differentiated carcinomas, 8 undifferentiated carcinomas) were immunostained with antibodies against occludin and claudin-1, -4 and -7. Occludin was mainly expressed in the form of intracytoplasmic vesicles, whereas all claudins tested exhibited membranous immunostaining. Thirteen out of 15 follicular adenomas, 10/15 follicular carcinomas, 24/26 papillary carcinomas, 15/16 papillary microcarcinomas, 1/8 medullary carcinomas, 2/3 poorly differentiated carcinomas and 2/8 undifferentiated carcinomas exhibited claudin-1 expression, whereas claudin-4 was expressed in 13/15, 12/15, 23/26, 13/16, 7/8, 2/3 and 2/8 of the tumors, respectively, and claudin-7 expression was found in 67, 33, 73, 69, 25, 0 and 13% of the cases, respectively. Occludin was expressed in 100% follicular adenomas, 80% follicular carcinomas, 96% papillary carcinomas, 50% papillary microcarcinomas, 50% medullary carcinomas, 33% poorly differentiated carcinomas and 88% undifferentiated carcinomas. Occludin expression was reduced in papillary microcarcinomas, medullary carcinomas and poorly differentiated carcinomas. All claudins exhibited reduced expression in undifferentiated carcinomas. Claudin-1 was additionally reduced in medullary carcinomas and claudin-7 in follicular, medullary and poorly differentiated carcinomas. A correlation between loss of claudin-1 expression and worse disease-free survival was noted on univariate analysis. Dedifferentiation of the thyroid carcinomas is accompanied by reduction in claudin-1, -4 and -7 expression. A differential expression of tight junction proteins in the different histologic types of thyroid gland is noted. Additionally, claudin-1 expression may be an important prognostic indicator of recurrence in thyroid carcinomas.

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“…Claudin 1,4 and 7 are important members of this Claudin protein family and their expressions show alterations in many different malignancies such as oesophageal, gastric, biliary (Usami et al, 2006;Agarwal et al, 2009;Kwon et al, 2011), ovarian (Kwon et al, 2010), endometrial (Pan et al, 2007;Knoecny et al, 2008), bladder (Boireau et al, 2007;Szekely et al, 2011) renal (Lechpammer et al, 2008), prostate (Landers et al, 2008), breast (Kominsky et al, 2003), cholangiocarcinoma (Bunthot et al, 2012) and pancreatic cancer.…”

Section: Introductionmentioning

confidence: 99%

Expression Pattern and Prognostic Significance of Claudin 1, 4 and 7 in Pancreatic Cancer

Ali̇kanoğlu¹,

Gündüz²,

Demirpençe³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Background: Tight junctions (TJs) organise paracellular permeability and they have an important role in epithelial and endothelial cell polarity and permanence of barrier function. It has been demonstrated that the Claudin family constitutes an important component of them. In this study, we assessed expression patterns of of Claudin1, 4 and 7 and whether they have any relation with prognosis in patients with pancreatic cancer. Materials and Methods: Expression patterns of Claudin 1,4 and 7 were examined by immunohistochemistry in 25 patients with a histopathological diagnosis of pancreatic cancer using a semiquantitative scoring of the extent and intensity of staining. After grouping the staining scores as low (final score 0-2) and high (final score 3-9) the relation between expression of Claudin 1,4 and 7 and survival was evaluated. Results: There was no significant relation between expression of Claudin 1,4 and 7 and gender and stage. No statistically significant relation was found between Claudin 1 and 4 expression and survival whereas a statistically significant relation was found between decrease in Claudin 7 expression and decrease in survival. Conclusions: Claudins have important functions other than their popular function known as adhesion. Supporting this hypothesis, we found a statistically significant relationship between increased Claudin 7 expression and increased survival time, and this suggests that Claudin 7 may exert different tumorigenic effects in pancreatic cancer other than its wellknown adhesion effect.

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Reduced expression of claudin-7 correlates with invasion and metastasis in squamous cell carcinoma of the esophagus

Cited by 136 publications

References 24 publications

Functional Characterization of a Novel BRCA1-Null Ovarian Cancer Cell Line in Response to Ionizing Radiation

Functional Characterization of a Novel BRCA1-Null Ovarian Cancer Cell Line in Response to Ionizing Radiation

Tight junctions in thyroid carcinogenesis: diverse expression of claudin-1, claudin-4, claudin-7 and occludin in thyroid neoplasms

Expression Pattern and Prognostic Significance of Claudin 1, 4 and 7 in Pancreatic Cancer

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