Reduced expression levels of the death-associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma

Zhai, Jianwen; Yang, Xiaogang; Qi, Qingbin; Hu, Jigang; Wang, Qiaomei

doi:10.3892/etm.2013.916

Cited by 9 publications

(4 citation statements)

References 11 publications

(9 reference statements)

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“…DAPK1 , as a classical anti-oncogene, has been demonstrated to play an important role in the development, progression and metastasis of tumors[ 7 ]. Down-regulation of DAPK1 expression has been correlated with the severity of malignancy and lymph node metastasis in various cancers including lung cancer[ 37 ], urinary tract carcinoma[ 38 ], and esophageal squamous cell carcinoma[ 39 ]. It has been shown that DAPK1 can influence cell survival and apoptosis by activating the mammalian target of rapamycin complex1 (mToRC1)[ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Correlation of DAPK1 methylation and the risk of gastrointestinal cancer: A systematic review and meta-analysis

et al. 2017

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ObjectiveOne of the critical mechanisms of gastrointestinal cancer pathogenesis is the silencing of death associated protein kinase 1 (DAPK1), which could be caused by aberrant methylation of the promoter. However, the relationship between DAPK1 methylation and the risk of gastrointestinal cancer is still controversial. Hence, we conducted this study to determine the potential correlation.MethodsEligible publications were searched in the Pubmed, Embase, and Cochrane Library through November 2016 according to the inclusion criteria and exclusion criteria. Revman 5.3 and Stata 12.0 software were used to analyze the relevant data regarding the association between the frequency of DAPK1 methylation and gastrointestinal cancer.ResultsA total of 22 studies with 2406 patients were included in this meta analysis. Methylation of DAPK1 was positively related with the risk of gastrointestinal cancer (odds ratio [OR] = 5.35, 95% confidence interval [CI]: 2.76–10.38, P<0.00001, random effects model). The source of heterogeneity was analyzed by sensitivity analysis and subgroup analysis. After omitting one heterogeneous study, the I2 decreased and the OR increased in pooled analysis. Also, the heterogeneity decreased most significantly in the subgroup of studies that had a sample size of less than 60 cases. Then, the correlations between DAPK1 methylation and clinicopathological features of gastrointestinal cancer were assessed. DAPK1 methylation was positively correlated with the lymph node (N) stage (positive vs. negative, OR = 1.45, 95%CI: 1.01–2.06, P = 0.04, fixed effects model) and poor differentiation (OR = 1.55, 95%CI: 1.02–2.35, P = 0.04, fixed effects model) in gastric cancer, and the association was significant among Asian patients. However, among cases of gastrointestinal cancer, the association between DAPK1 methylation and tumor (T) stage, N stage, distant metastasis (M) stage, and cancer differentiation were not statistically significant.ConclusionsDAPK1 methylation is a potential biomarker for the early diagnosis of gastrointestinal cancer. Further analysis of the clinicopathological features indicated that aberrant methylation of DAPK1 is positively associated with the tumorigenesis of gastrointestinal cancer, and metastasis of gastric cancer.

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Section: Discussionmentioning

confidence: 99%

Correlation of DAPK1 methylation and the risk of gastrointestinal cancer: A systematic review and meta-analysis

et al. 2017

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“…After blocking with 10% goat serum, the slides were incubated overnight at 4°C with primary monoclonal antibodies for RAB1A (1:200, Cell Signaling Technology). After several rinses in phosphate-buffered saline, the slides were incubated in the biotinylated secondary antibodies, and antibodies were fixed using the streptavidin-peroxidase (SP) immunohistochemical method (22). The semi-quantitative results were analyzed according to the staining intensity and percentage of positively labeled cells.…”

Section: Methodsmentioning

confidence: 99%

miR‑139‑3p suppresses the invasion and migration properties of breast cancer cells by targeting RAB1A

Zhang

Wang

et al. 2019

Oncol Rep

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Accumulated evidence indicates that aberrant microRNAs (miRNAs) expression plays an important role in the initiation and progression of various cancers, including breast cancer. Previous studies suggested that miR-139-3p might serve as a tumor suppressor and is downregulated in several cancer types. However, the expression patterns and exact role of miR-139-3p in breast cancer remain to be elucidated. In this study, we aimed to analyze the effect of miR-139-3p on the progression of breast cancer and the mechanism involved. Through bioinformatics analysis and in vitro experimental studies, we found that miR-139-3p was decreased in breast cancer tissues and cell lines, and decreased miR-139-3p is associated with a poor prognosis in breast cancer patients. Overexpression of miR-139-3p by transfection significantly inhibits cell proliferation, migration, and invasion of breast cancer cells. Bioinformatics analysis and luciferase reporter gene assay confirmed that RAB1A was a potential target of miR-139-3p. Furthermore, overexpression of RAB1A counteracted the suppressing effects of miR-139-3p on breast cancer cell migration, invasion and epithelial-to-mesenchymal transition (EMT). Taken together, these data suggest that miR-139-3p plays a tumor suppressive role in breast cancer by targeting RAB1A and may serve as a potential new biomarker for breast cancer prognosis.

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“…These 2 tissue blocks were used for immunohistochemical analysis. The immunohistochemical SP method [ 6 ] was used to detect the expression of NLRP3 and caspase-1 in placental tissues. The positive NLRP3 and caspase-1 expression slices provided by the reagent company were used as the positive controls, and PBS was used as a negative control instead of an antibody.…”

Section: Methodsmentioning

confidence: 99%

Expression and Clinical Significance of NOD-Like Receptor Protein 3 (NLRP3) and Caspase-1 in Fetal Membrane and Placental Tissues of Patients with Premature Rupture of Membrane

Zhu¹,

He²,

Ma³

et al. 2018

Med Sci Monit

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BackgroundIn this study, we aimed to investigate the expression of NOD-like receptor protein 3 (NLRP3) and caspase-1 in fetal membrane and placental tissues of patients with premature rupture of membrane (PROM), and to explore their role in PROM.Material/MethodsNinety women participated in this study: a control group of 30 healthy pregnant women, 30 with PPROM, and 30 with TPROM. Immunohistochemistry streptavidin-peroxidase (SP) assay was used to detect the protein expression of NLRP3 and caspase-1 in the fetal membrane and placental tissues. RT-PCR was used to detect the mRNA expression of NLRP3 and caspase-1 in fetal membrane and placental tissues.ResultsThe results of SP showed that NLRP3 and caspase-1 were mainly expressed in the cytoplasm of epithelial cells, mesenchymal cells, and trophoblast cells in fetal membranes, and the cytoplasm of placental syncytiotrophoblasts and vascular endothelial cells in placental tissues. The expression of NLRP3 and caspase-1 in the TPROM group was significantly higher than that in the PPROM group and control group (p<0.05), and there was a significant difference between the PPROM group and the control group. The results of RT-PCR showed that the mRNA expression level of NLRP3 and caspase-1 in the TPROM group was significantly higher than that in the PPROM group and control group (p<0.05), and the expression of NLRP3 mRNA and caspase-1 mRNA in the PPROM group was significantly different from that in the control group (p>0.05).ConclusionsThe increased expression of NLRP3 and caspase-1 in fetal membrane and placental tissues may be associated with the development of PROM.

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Reduced expression levels of the death-associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma

Cited by 9 publications

References 11 publications

Correlation of DAPK1 methylation and the risk of gastrointestinal cancer: A systematic review and meta-analysis

Correlation of DAPK1 methylation and the risk of gastrointestinal cancer: A systematic review and meta-analysis

miR‑139‑3p suppresses the invasion and migration properties of breast cancer cells by targeting RAB1A

Expression and Clinical Significance of NOD-Like Receptor Protein 3 (NLRP3) and Caspase-1 in Fetal Membrane and Placental Tissues of Patients with Premature Rupture of Membrane

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