Reduced dynamic loads due to hip dislocation induce acetabular cartilage degeneration by IL-6 and MMP3 via the STAT3/periostin/NF-κB axis

Nakamura, Yutaka; Saitou, Mitsuru; Komura, Shingo; Matsumoto, Kazu; Ogawa, Hiroyasu; Miyagawa, Takaki; Saitou, Takashi; Imamura, Takeshi; Imai, Yuuki; Takayanagi, Hiroshi; Akiyama, Haruhiko

doi:10.1038/s41598-022-16585-6

Cited by 8 publications

(4 citation statements)

References 48 publications

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“…Because mucin-1 was upregulated in Nthy-ori 3-1 cells only in the RPM samples (not by DEX alone), a hypoxia-induced effect, which has been described in other epithelial cells for other mucins [ 81 ], can be excluded here. One possibility could be RPM-induced expression of MUC1 via STAT3, as recently described in chondrocytes [ 82 ]. Due to steric hindrance of mucin-1 and adhesion proteins, overexpressed mucin-1 has an anti-adhesion effect on cells [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

Dexamethasone Selectively Inhibits Detachment of Metastatic Thyroid Cancer Cells during Random Positioning

Melnik

Cortés-Sánchez

Sandt

et al. 2023

Cancers

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We recently reported that synthetic glucocorticoid dexamethasone (DEX) is able to suppress metastasis-like spheroid formation in a culture of follicular thyroid cancer (FTC)-133 cells cultured under random positioning. We now show that this inhibition was selective for two metastatic thyroid carcinoma cells, FTC-133 and WRO, whereas benign Nthy-ori 3-1 thyrocytes and recurrent ML-1 follicular thyroid cancer cells were not affected by DEX. We then compare Nthy-ori 3-1 and FTC-133 cells concerning their adhesion and mechanosignaling. We demonstrate that DEX disrupts random positioning-triggered p38 stress signaling in FTC-133 cells, thereby antagonizing a variety of biological functions. Thus, DEX treatment of FTC-133 cells is associated with increased adhesiveness, which is mainly caused by the restored, pronounced formation of a normal number of tight junctions. Moreover, we show that Nthy-ori 3-1 and ML-1 cells upregulate the anti-adhesion protein mucin-1 during random positioning, presumably as a protection against mechanical stress. In summary, mechanical stress seems to be an important component in this metastasis model system that is processed differently by metastatic and healthy cells. The balance between adhesion, anti-adhesion and cell–cell connections enables detachment of adherent human cells on the random positioning machine—or not, allowing selective inhibition of thyroid in vitro metastasis by DEX.

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Section: Discussionmentioning

confidence: 99%

Dexamethasone Selectively Inhibits Detachment of Metastatic Thyroid Cancer Cells during Random Positioning

Melnik

Cortés-Sánchez

Sandt

et al. 2023

Cancers

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“…[18] Moreover, IL-6 can induce the expression of POSTN through the IL-6-STAT3 pathway. [16,19] We therefore examined whether GMF𝛽 could regulate the expression of POSTN through the IL-6-STAT3 pathway. We used western blot to detect the protein expression of IL-6, STAT3, and p-STAT3, key molecules of the IL-6/STAT3 signaling pathway.…”

Section: Gmf𝜷 Regulated the Expression Of Postn Through Il-6-stat3 Pa...mentioning

confidence: 99%

Preliminary Mechanism of Glial Maturation Factor β on Pulmonary Vascular Remodeling in Pulmonary Arterial Hypertension

Li,

Shi,

Yan

et al. 2024

Advanced Biology

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Recent evidence suggests that glia maturation factor β (GMFβ) is important in the pathogenesis of pulmonary arterial hpertension (PAH), but the underlying mechanism is unknown. To clarify whether GMFβ can be involved in pulmonary vascular remodeling and to explore the role of the IL‐6‐STAT3 pathway in this process, the expression of GMFβ in PAH rats is examined and the expression of downstream molecules including periostin (POSTN) and interleukin‐6 (IL‐6) is measured using real‐time quantitative polymerase chain reaction (RT‐qPCR) and western blot analysis. The location and expression of POSTN is also tested in PAH rats using immunofluorescence. It is proved that GMFβ is upregulated in the lungs of PAH rats. Knockout GMFβ alleviated the MCT‐PAH by reducing right ventricular systolic pressure (RVSP), mean pulmonary arterial pressure (mPAP), and pulmonary vascular remodeling. Moreover, the inflammation of the pulmonary vasculature is ameliorated in PAH rats with GMFβ absent. In addition, the IL‐6‐STAT3 signaling pathway is activated in PAH; knockout GMFβ reduced POSTN and IL‐6 production by inhibiting the IL‐6‐STAT3 signaling pathway. Taken together, these findings suggest that knockout GMFβ ameliorates PAH in rats by inhibiting the IL‐6‐STAT3 signaling pathway.

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“…This research proves that the tissue senescence does not depend strictly on inflammation (in muscle tissues at least). Nakamura et al [28] examined a murine model of cartilage destruction. The obtained results suggested that a joint surface load reduction activates cartilage degeneration by NF-κB signaling.…”

Section: Kinesiotherapy-treatment With Movementmentioning

confidence: 99%

Why Does Rehabilitation Not (Always) Work in Osteoarthritis? Does Rehabilitation Need Molecular Biology?

Zdziechowski

Gluba-Sagr

Rysz

et al. 2023

IJMS

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Osteoarthritis (OA) is a common disease among the human population worldwide. OA causes functional impairment, leads to disability and poses serious socioeconomic burden. The rehabilitation offers a function-oriented method to reduce the disability using diverse interventions (kinesiotherapy, physical therapy, occupational therapy, education, and pharmacotherapy). OA as a widespread disease among elderly patients is often treated by rehabilitation specialists and physiotherapists, however the results of rehabilitation are sometimes unsatisfactory. The understanding of molecular mechanisms activated by rehabilitation may enable the development of more effective rehabilitation procedures. Molecular biology methods may prove crucial in rehabilitation as the majority of rehabilitation procedures cannot be estimated in double-blinded placebo-controlled trials commonly used in pharmacotherapy. This article attempts to present and estimate the role of molecular biology in the development of modern rehabilitation. The role of clinicians in adequate molecular biology experimental design is also described.

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Reduced dynamic loads due to hip dislocation induce acetabular cartilage degeneration by IL-6 and MMP3 via the STAT3/periostin/NF-κB axis

Cited by 8 publications

References 48 publications

Dexamethasone Selectively Inhibits Detachment of Metastatic Thyroid Cancer Cells during Random Positioning

Dexamethasone Selectively Inhibits Detachment of Metastatic Thyroid Cancer Cells during Random Positioning

Preliminary Mechanism of Glial Maturation Factor β on Pulmonary Vascular Remodeling in Pulmonary Arterial Hypertension

Why Does Rehabilitation Not (Always) Work in Osteoarthritis? Does Rehabilitation Need Molecular Biology?

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