“…analysis were performed as previously described (26). In order to detect bands of interest, primary antibodies directed against HSP70 (Abcam ab5439, 1:1,000), proliferating cell nuclear antigen (PCNA) (mouse monoclonal, sc-56, 1:500; Santa Cruz Biotechnology, Inc.) or β-actin (mouse monoclonal, A5441, 1:2,000; Sigma-Aldrich ® , St. Louis, MO, USA) were added to the nitrocellulose membranes followed by incubation (1:2,000) with an appropriate secondary goat anti-mouse (sc-2005) horseradish peroxidase-coupled antibody (Santa Cruz Biotechnology, Inc.).…”