Reduced bovine leukaemia virus proviral load in genetically resistant cattle

Mirsky, Michael; Olmstead, Colleen; Da, Yang; Lewin, Harris A.

doi:10.1046/j.1365-2052.1998.00320.x

Cited by 50 publications

(44 citation statements)

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“…Further bias might have been introduced by selecting the smaller flocks in the extensive system and those registered with the local milk-board among intensive flocks, but it is unlikely that either factor contributes greatly to explaining the enormous differences in MVV-seroprevalence in these two sheep production systems. Moreover, although certain alleles have been identified that confer goats and cows some degree of resistance to lentiviral infections [19,29] and there are indications of inheritable susceptibility and resistance to MVVseroconversion in sheep [6], there is no evidence or previous suggestions, that major differences in flock MVV-seroprevalence are attributable to genetic differences between sheep. Breed-associated differences in susceptibility to infection have been proposed.…”

Section: Discussionmentioning

confidence: 99%

Extensive rearing hinders Maedi-Visna Virus (MVV) infection in sheep

et al. 2006

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-Maedi-Visna Virus (MVV) seroprevalence and its relationship with housing and mode of rearing of replacement ewe-lambs was investigated in 38 non-randomly selected sheep-flocks in Spain. They included extensive lamb-producing Manchega cross-bred flocks raised almost permanently at pasture, semi-intensive Latxa dairy flocks housed 2-8 months/year and intensively raised Assaf dairy flocks housed most time and at higher stocking density in less ventilated buildings than other flocks. Most flocks raised replacement lambs naturally with their dams until weaning and as a separate flock thereafter until lambing at one year of age. Seroprevalence (95% confidence intervals) was 77%, 25% and 5% (4-6) in intensive, semi-intensive and extensive flocks, respectively and the median (interquartile range) flock-seroprevalence was 82% (66-94) in intensive flocks, 31% (14-31) in semi-intensive flocks and 4% (0-7) in extensive flocks. Seroprevalence was lowest in one year-old sheep and increased to flock levels during the year after introduction into the adult flock in most intensive flocks and more gradually in other flocks. Adult flock seroprevalence was associated with housing time but this relationship was not evident within a particular rearing system, indicating that other unknown factors are critical in horizontal MVV-transmission. Low seroprevalence in extensive flocks further supports previous indications that lactogenic MVV-infection is relatively inefficient and horizontal transmission is necessary to ensure long-term maintenance of MVV and this could explain that MVV has not been reported from countries with mainly extensively reared sheep such as Australia and New Zealand. Moreover, it indicates that MVV-control in extensive and semi-intensive flocks can be simple and inexpensive.Maedi-Visna Virus / sheep / seroprevalence / intensive and extensive rearing / Spain

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Section: Discussionmentioning

confidence: 99%

Extensive rearing hinders Maedi-Visna Virus (MVV) infection in sheep

et al. 2006

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“…In the present investigation we found a significant number of animals carrying this allele in our herd, compared to the population of Holstein in Argentina (TAKESHIMA et al, 2015); however, no association could be established between allele BoLA DRB3.2*1101 and BLV PVL. In the literature BoLA DRB3.2*0902 (or *11 determined by PCR-RFLP) allele was most strongly associated with PL resistance or LPVL profile in Holstein cattle (XU et al, 1993;ZANOTTI et al, 1996;MIRSKY et al, 1998;JULIARENA et al, 2008;PANEI et al, 2009;MIYASAKA et al, 2013). In the present investigation we did not find this association, which could be due to a lower frequency of presentation of *0902 allele in this herd in comparison with the Argentinean Holstein populations (4.21% vs 6.4%, respectively) (TAKESHIMA et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Related to the infection with BLV in Holstein breed, several studies have shown that the susceptibility to PL was associated with the presence of BoLA DRB3*0101, *1501 or 03, *1101 or 02, and *1201 (XU et al, 1993;ZANOTTI et al, 1996;MIRSKY et al, 1998;JULIARENA et al, 2008;PANEI et al, 2009;NIKBAKHT BRUJENI et al, 2016). In the present study, BoLA DRB3*1201 was the most significantly associated with HPVL (p = 0.0111), and this might be due to the fact that its frequency in the investigated population was about one third higher than in other Argentinean Holstein populations (11.21% vs 7.3%, respectively) (TAKESHIMA et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, most studies have focused on BoLA DRB3.2 allele polymorphisms in Holstein cattle, and their relationship with disease. Some alleles were associated with resistance to PL or to BLV-induced lymphosarcoma, or the capability of controlling the number of BLV-infected cells, while other alleles were associated with susceptibility to PL (XU et al, 1993;ZANOTTI et al, 1996;MIRSKY et al, 1998;PANEI et al, 2009;NIKBAKHT BRUJENI et al, 2016). When BLV-infected animals were characterized into two profiles of infection -high and low proviral load (HPVL and LPVL respectively) (JULIARENA et al, 2007), BoLA DRB3.2 *0902 allele was the strongest associated with LPVL profile, while *1501 or 03 allele showed a significant association with HPVL in carrier animals (JULIARENA et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads

Farías

Caffaro

Lendez

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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Bovine leukemia virus (BLV) is associated with the most common neoplastic disease of cattle. BLV has a silent dissemination in the herd due to infected cell exchange, thus the concentration of BLV-infected cells in blood should play a major role in the success of viral transmission. Genes from Bovine leukocyte antigen (BoLA), the MHC system of cattle, are associated with genetic resistance and susceptibility to a wide range of diseases, and also with production traits. Some BoLA DRB3.2 allele polymorphisms in Holstein cattle have been associated with resistance or susceptibility to BLV-disease development, or with proviral load (PVL). This investigation studied 107 BLV-infected Argentinean Holstein dairy cows, all of them belonging to one herd. PVL was analysed by qPCR and animals were classified as high proviral load (HPVL, N = 88) and low proviral load (LPVL, N = 19), and BoLA DRB3.2 alleles were genotyped. Alleles BoLA DRB3.2*1501 and *1201 were significantly associated with HPVL (p = 0.0230 and p = 0.0111 respectively), while allele BoLA DRB3.2*0201 was significantly associated with LPVL (p = 0.0030). The present study aims at contributing to the knowledge of the association between BoLA polymorphism and development of a BLV infection profile. Genes that best explain the PVL in this population resulted BoLA DRB3.2*0201 (as a protection factor) and *1501 (as a risk factor). Allelic differences may play an important role in the development of effective immune responses. A better understanding of how BoLA polymorphism contributes to these responses and the establishment of a BLV status is desirable to schedule and evaluate control measures. Keywords: BLV. Proviral load. BoLA DRB3 polymorphism. ResumoO vírus da leucemia bovina (BLV) está associado à doença neoplásica mais comum do gado bovino. O BLV tem uma disseminação silenciosa no rebanho devido à troca de células infectadas, assim, a concentração de células BLV infectadas no sangue deve desempenhar um papel importante no sucesso da transmissão viral. Os genes do antígeno leucocitário bovino (BoLA), sistema MHC do gado bovino, estão associados à resistência genética e à susceptibilidade a uma ampla gama de doenças, bem como às características da produção. Alguns polimorfismos de alelos de BoLA DRB3.2 em bovinos Holstein têm sido associados à resistência ou susceptibilidade ao desenvolvimento da doença BLV, ou com carga proviral (PVL). Esta investigação avaliou 107 vacas leiteiras da raça Holstein argentina infectadas com BLV e pertencentes a um único rebanho. A PVL foi analisada por qPCR, os animais foram classificados em alta carga proviral (HPVL, N = 88) e baixa carga proviral (LPVL, N = 19) | 216Braz.

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“…However, the association between immune response and these ovine MHC-DR molecules remains unknown. In BLV infection, several previous studies (23,26,48) revealed the presence of the amino acids Glu-Arg (ER) at positions 70-71 of the bovine leukocyte antigen (BoLA)-DRβ chain associated with resistance to PL in cattle (48). Furthermore, it appeared that BoLA-DRB3 alleles encoding Glu, Arg and Val at positions 74, 77 and 78 of the BoLA-DRβ chain might be related to resistance to tumor development (1).…”

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confidence: 99%

The Influence of Ovine MHC Class IIDRB1Alleles on Immune Response in Bovine Leukemia Virus Infection

Konnai

Takeshima

Tajima

et al. 2003

Microbiology and Immunology

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We have reported previously that the alleles of the ovine leukocyte antigen (OLA)‐DRB1 gene that encode the Arg‐Lys (RK) motif and the Ser‐Arg (SR) motif at positions β70/71 of the OLA‐DRβ1 domain are associated with resistance and susceptibility, respectively, to development of bovine leukemia virus (BLV)‐induced ovine lymphoma. Here, to investigate the different immune response in sheep that carried alleles associated with resistance and susceptible for 30 weeks after infection with BLV, we selected sheep that had the RK/RK or SR/SR genotype among the 52 sheep analyzed by polymerase chain reaction‐restriction fragment length polymorphism and DNA sequencing of PCR product for the OLA‐DRB1 exon 2 and infected them with BLV. Although the number of BLV‐infected cells and virus titer had been maintaining low levels throughout the experimental period, the sheep with the RK/RK genotype could induce expansion of CD5− B‐cells and rapid production of neutralizing antibody in the early phase of infection. The level of incorporation of [3H]thymidine by peripheral blood mononuclear cells from the sheep with RK/RK genotype gave a strong response to BLV virion antigen and synthetic antigenic peptides that corresponded to T‐helper epitope of the BLV envelope glycoprotein gp51. In contrast, the sheep with SR/SR genotype showed a strong response to BLV virion antigen and synthetic antigenic peptides that corresponded to T‐cytotoxic and B‐cell epitopes. In such cases, the animals with the RK/RK strongly expressed IFN‐7, the animals with SR/SR genotype strongly expressed IL‐2. To determine the proliferating cells, we tried a blocking assay with monoclonal antibodies such as anti‐CD4, ‐CD8 and ‐DR molecule. We found that these proliferating cells were MHC‐restricted CD4+ T‐cells.

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Reduced bovine leukaemia virus proviral load in genetically resistant cattle

Cited by 50 publications

References 0 publications

Extensive rearing hinders Maedi-Visna Virus (MVV) infection in sheep

Extensive rearing hinders Maedi-Visna Virus (MVV) infection in sheep

A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads

The Influence of Ovine MHC Class IIDRB1Alleles on Immune Response in Bovine Leukemia Virus Infection

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