Reduced bacterial adhesion on titanium surfaces micro-structured by ultra-short pulsed laser ablation

Döll, Katharina; Fadeeva, Elena; Stumpp, Nico; Grade, Sebastian; Chichkov, Boris N.; Stiesch, Meike

doi:10.1515/bnm-2015-0024

Cited by 19 publications

(13 citation statements)

References 15 publications

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“…Therefore, the question has to be asked whether the surface modifications assessed here are of unambiguous benefit for their application on implantable devices. Interestingly, a cleansing or decontaminating effect of laser pretreatment is well described and suggests that, despite the larger surface area available for bacterial adhesion, biofilm formation should not be stimulated by nanostructured Ti surfaces. However, this needs to be confirmed by suitable follow‐up experiments including in vivo studies.…”

Section: Discussionmentioning

confidence: 99%

Laser‐Induced Nanostructures on Titanium Surfaces as Developed in the Aeronautics and Space Industry Foster Osteoblast Activity and Function In Vitro

Jablonski

Wedemeyer²,

Rekasi

et al. 2018

Adv Materials Inter

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The stability of orthopedic implants is governed by the interaction between the material's surface and bone cells. Nanosized structures seem to be promising in supporting the osseointegration of total joint replacements. In order to analyze whether nanostructured titanium surfaces, as generated by a short‐pulsed Nd:YVO4 laser irradiation process developed in the aeronautics and space industry as a “cold ablation” process, can be useful for orthopedic applications, their impact on bone cells is evaluated in vitro. Cell spreading and morphology on nanostructured titanium niobium nitride or titanium plasma‐sprayed surfaces are comparable to osteoblast behavior on corresponding untreated titanium or cells grown on cover glass. However, on the nanostructured surfaces cell numbers appear to be reduced. Nonetheless, cell viability is not affected by laser pretreatment. Interestingly, osteoblast proliferation on nanostructured titanium is inhibited to the benefit of an increased production of osteoblast‐specific proteins such as bone alkaline phosphatase, osteocalcin, or procollagen. Furthermore, an enhanced mineralization of cells grown on nanostructured surfaces is confirmed in terms of an elevated hydroxyapatite deposition. Laser irradiation leads to an additional nanostructure on microstructured titanium surfaces and might positively affect the biocompatibility and ingrowth of prostheses made from the corresponding biomaterials.

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Section: Discussionmentioning

confidence: 99%

Laser‐Induced Nanostructures on Titanium Surfaces as Developed in the Aeronautics and Space Industry Foster Osteoblast Activity and Function In Vitro

Jablonski

Wedemeyer²,

Rekasi

et al. 2018

Adv Materials Inter

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“…For the three dimensional reconstruction each z-stack was separately loaded in the The stepwise calculation and depiction of the relevant fractions is shown in Figure 1. The first step (I) was to calculate the total volume of the living, dead and colocalized biomass per section (in µm 3 /A) using the corresponding fluorescence canals as published by Doll et al [23,29]. After determining the volume for these three fractions, the second step (II) was to distinguish between bacterial and euaryotic cellular biomass in the fractions total living biomass and total colocalized biomass.…”

Section: Live Bacteria =Live Total -(Live Eukaryotic Cells +Colocalismentioning

confidence: 99%

“…However, the development of the ideal antibacterial implant surface is very challenging, because these surfaces have to prevent bacterial adhesion while simultaneously keeping adequate biocompatibility and promoting osseointegration [19,22]. Surface modification is one approach for antibacterial implant surfaces and has shown a bacterial repellent effect in vitro [23]. To test nano and micro structures in vivo, suitable animal models and evaluation techniques need to be available.…”

Section: Introductionmentioning

confidence: 99%

A New Quantification Method of Bacterial Adherence on Implant Surfaces in an Implant Associated Infection Rat Model

Ml¹,

Angrisani²,

Hegermann³

et al. 2018

J Med Diagn Meth

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Objective: Implant infections in elective orthopedic surgery are still a clinically relevant problem with devastating consequences for the patient. Thereof results a great need for new strategies to prevent implant infections. Functionalization of implant surfaces to reduce the microbial adherence show great potential in vitro, but have to be tested in suitable models in vivo. Proper evaluation methods of the bacterial load on the implant surface are important for their evaluation. Up to now, the simultaneous assessment of the quantity and morphology of the bacterial infection in vivo was not performed. Methods: Cubic Ti90/Al6/V4-rods were inserted in the tibia of Lewis rats and infected with Staphylococcus aureus strain 36/07 in different concentrations. After 21 days, explanted implants were stained for living and dead cells. The bacterial surface colonization was analyzed by confocal microscopy and assessed semi quantitatively via two different approaches (spot method and volume method) using the software Imaris ×64. Furthermore the bacterial morphology was evaluated. The results were compared to radiographic and histological changes. Results: The new semi quantitative CLSM evaluation to assess the bacterial biomass on implant surfaces was successfully implemented. Both methods gave equivalent results. The results of the morphologic assessment of the bacterial colonization were similar to those of the quantification. A tendency towards increasing bacterial biomass and biofilm formation on the implant surface was observed with decreasing infection concentrations. In contrast, histologic and radiographic assessment as well as the relative tibial bone weight revealed more severe changes for higher inoculation concentrations. Conclusion: In combination with the morphological assessment of the bacterial appearance this CLSM based evaluation is a suitable tool to assess the bacterial load on the implant surface. Combined with radiographical and histological evaluation of bone alterations, this model is appropriate for the evaluation of new implant surfaces.

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“…and rolling angle (RA) less than 10°, have attracted extensive attention owing to their great significance in academic research and potential in practice [5][6] . These super-hydrophobic surfaces play a significant role in various practical applications, such as self-cleaning, corrosion inhibition, anti-bacteria, oil/water separation and drag reduction [7][8][9][10][11][12] . Many super-hydrophobic surfaces can be observed in nature and it is well accepted that the super-hydrophobicity is governed by the combination of hierarchical micro/nano structures and surface chemistry [13][14] .…”

mentioning

confidence: 99%

Hybrid Laser Ablation and Chemical Modification for Fast Fabrication of Bio-inspired Super-hydrophobic Surface with Excellent Self-cleaning, Stability and Corrosion Resistance

2019

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Reduced bacterial adhesion on titanium surfaces micro-structured by ultra-short pulsed laser ablation

Cited by 19 publications

References 15 publications

Laser‐Induced Nanostructures on Titanium Surfaces as Developed in the Aeronautics and Space Industry Foster Osteoblast Activity and Function In Vitro

Laser‐Induced Nanostructures on Titanium Surfaces as Developed in the Aeronautics and Space Industry Foster Osteoblast Activity and Function In Vitro

A New Quantification Method of Bacterial Adherence on Implant Surfaces in an Implant Associated Infection Rat Model

Hybrid Laser Ablation and Chemical Modification for Fast Fabrication of Bio-inspired Super-hydrophobic Surface with Excellent Self-cleaning, Stability and Corrosion Resistance

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