Reduced Anthocyanins in Petioles codes for a GST anthocyanin transporter that is essential for the foliage and fruit coloration in strawberry

Luo, Huifeng; Dai, Cheng; Li, Yongping; Feng, Jia; Liu, Zhongchi; Kang, Chunying

doi:10.1093/jxb/ery096

Cited by 137 publications

(165 citation statements)

References 65 publications

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“…Heterologous overexpression of VviGST4 ( Vitis vinifera ) and AcGST1 ( Actinidia chinensis ) in Arabidopsis tt19 mutant can functionally complement both the anthocyanin‐less phenotype in plant and the PA‐deficient phenotype in seed coat (Liu et al , ; Pérez‐Díaz et al , ). However, in this study, PpGST1 only possessed the capacity to functionally complement the anthocyanin‐less phenotype of Arabidopsis tt19 mutant but not the PA‐deficient phenotype in the seed coat, which was similar to the result of An9 ( Petunia hybrida ), LcGST4 ( Litchi chinensis ), RAP ( Fragaria ananassa ), CsGSTF1 ( Camellia sinensis ) and MdGSTF6 ( Malus domestica ) in the tt19 complementation assay (Hu et al , ; Jiang et al , ; Kitamura et al , ; Luo et al , ; Wei et al , ). These data indicated that PpGST1 plays a pivotal role in anthocyanin transport and differed from AtTT19 , VviGST4 and AcGST1 which also participate in seed deposition of PAs.…”

Section: Discussionsupporting

confidence: 73%

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PpGST1, an anthocyanin‐related glutathione S‐transferase gene, is essential for fruit coloration in peach

Zhao

Dong

Zhu

et al. 2019

Plant Biotechnology Journal

103

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Summary Anthocyanins have crucial biological functions and affect quality of horticultural produce. Anthocyanins accumulate in ripe peach fruit; differential accumulation is observed in deep coloured cultivar ‘Hujingmilu’ and lightly pigmented cultivar ‘Yulu’. The difference was not fully explained by accumulation of total flavonoids and expression of anthocyanin biosynthetic genes. Expression analysis was conducted on a glutathione S‐transferase gene (PpGST1), and it was found that the expression correlated well with anthocyanin accumulation in peach fruit tissues. Functional complementation of the Arabidopsis tt19 mutant indicated that PpGST1 was responsible for transport of anthocyanins but not proanthocyanidins. PpGST1 was localized in nuclei and the tonoplast, including the sites at which anthocyanin vacuolar sequestration occurred. Transient overexpression of PpGST1 together with PpMYB10.1 in tobacco leaves and peach fruit significantly increased anthocyanin accumulation as compared with PpMYB10.1 alone. Furthermore, virus‐induced gene silencing of PpGST1 in a blood‐fleshed peach not only resulted in a reduction in anthocyanin accumulation but also a decline in expression of anthocyanin biosynthetic and regulatory genes. Cis‐element analysis of the PpGST1 promoter revealed the presence of four MYB binding sites (MBSs). Dual‐luciferase assays indicated that PpMYB10.1 bound to the promoter and activated the transcription of PpGST1 by recognizing MBS1, the one closest to the ATG start codon, with this trans‐activation being stronger against the promoter of deep coloured ‘Hujingmilu’ compared with lightly coloured cultivar ‘Yulu’. Altogether, our data provided molecular evidence supporting coordinative regulatory roles of PpGST1 and PpMYB10.1 in anthocyanin accumulation in peach.

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Section: Discussionsupporting

confidence: 73%

“…For example, LcGST4 was involved in anthocyanin transport in litchi (Hu et al , ). In strawberry, RAP encodes a GST protein critical for anthocyanin accumulation in fruit and foliage (Luo et al , ). In grapevine, VviGST3 is related to PA transport while VviGST4 is pivotal for both anthocyanin and PA accumulation (Pérez‐Díaz et al , ).…”

Section: Introductionmentioning

confidence: 99%

PpGST1, an anthocyanin‐related glutathione S‐transferase gene, is essential for fruit coloration in peach

Zhao

Dong

Zhu

et al. 2019

Plant Biotechnology Journal

103

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“…Other TFs for which expression was correlated with flavonoid metabolism, such as TCP1 ( FvH4_6g16170 ), ZFN2 (FvH4_7g01060) and DIV (FvH4_6g11950) (Pillet et al , ), were down‐regulated in transgenic red receptacles (Table S2). In contrast, other genes associated with anthocyanin production in strawberry, such as the four members of the RAP family ( FvH4_1g27460 , FvH4_6g38760 , FvH4_2g25200 , FvH4_2g25210 ), encoding GST anthocyanin transporters (Luo et al , ), were up‐regulated in white receptacles of the silenced lines.…”

Section: Metabolic Changes In the Receptacles Of Famads9‐silenced Linmentioning

confidence: 92%

Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Vallarino

Merchante

Sánchez-Sevilla

et al. 2019

Plant Biotechnology Journal

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FaMADS9 is the strawberry (Fragaria x ananassa) gene that exhibits the highest homology to the tomato (Solanum lycopersicum) RIN gene. Transgenic lines were obtained in which FaMADS9 was silenced. The fruits of these lines did not show differences in basic parameters, such as fruit firmness or colour, but exhibited lower Brix values in three of the four independent lines. The gene ontology MapMan category that was most enriched among the differentially expressed genes in the receptacles at the white stage corresponded to the regulation of transcription, including a high percentage of transcription factors and regulatory proteins associated with auxin action. In contrast, the most enriched categories at the red stage were transport, lipid metabolism and cell wall. Metabolomic analysis of the receptacles of the transformed fruits identified significant changes in the content of maltose, galactonic acid‐1,4‐lactone, proanthocyanidins and flavonols at the green/white stage, while isomaltose, anthocyanins and cuticular wax metabolism were the most affected at the red stage. Among the regulatory genes that were differentially expressed in the transgenic receptacles were several genes previously linked to flavonoid metabolism, such as MYB10, DIV, ZFN1, ZFN2, GT2, and GT5, or associated with the action of hormones, such as abscisic acid, SHP, ASR, GTE7 and SnRK2.7. The inference of a gene regulatory network, based on a dynamic Bayesian approach, among the genes differentially expressed in the transgenic receptacles at the white and red stages, identified the genes KAN1, DIV, ZFN2 and GTE7 as putative targets of FaMADS9. A MADS9‐specific CArG box was identified in the promoters of these genes.

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“…Polyphenolic compounds are known to strongly interact with proteins sometimes without any specificity (Adamczyk et al, 2017), while the interaction of GST293 with the flavonoid-derived compounds could be more specific. Indeed, it has already been shown that plant phi GSTs are able to conjugate glutathione to cyanidin 3-glucoside to form a conjugate that is then transported to the vacuole via tonoplast Mg-ATP-requiring glutathione pumps (Alfenito et al, 1998;Luo et al, 2018). The interaction of GST293 will be studied more in detail but one can hypothesize that it could be involved in the transport and the solubilization of such compounds in the context of wood extractive detoxification.…”

Section: Intradiol Ring-cleavage Dioxygenasesmentioning

confidence: 99%

Oak extractive‐induced stress reveals the involvement of new enzymes in the early detoxification response of Phanerochaete chrysosporium

Fernández-González

Valette

Kohler

et al. 2018

Environmental Microbiology

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Extensive evidence showed that the efficiency of fungal wood degradation is closely dependent on their ability to cope with the myriad of putative toxic compounds called extractives released during this process. By analysing global gene expression of Phanerochaete chrysosporium after short oak extractive treatment (1, 3 and 6 h), we show that the early molecular response of the fungus concerns first mitochondrial stress rescue followed by the oxidation and finally conjugation of the compounds. During these early responses, the lignolytic degradative system is not induced, rather some small secreted proteins could play an important role in cell protection or signaling. By focusing on the functional characterization of an hitherto uncharacterized glutathione transferase, we show that this enzyme interacts with wood molecules suggesting that it could be involved in the detoxification of some of them, or act as a scavenger to prevent their cytosolic toxicity and favour their transport.

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Reduced Anthocyanins in Petioles codes for a GST anthocyanin transporter that is essential for the foliage and fruit coloration in strawberry

Abstract: Reduced Anthocyanins in Petioles (RAP) encodes a glutathione S-transferase anthocyanin transporter that is essential for the foliage and fruit coloration in strawberry.

Cited by 137 publications

References 65 publications

PpGST1, an anthocyanin‐related glutathione S‐transferase gene, is essential for fruit coloration in peach

PpGST1, an anthocyanin‐related glutathione S‐transferase gene, is essential for fruit coloration in peach

Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Oak extractive‐induced stress reveals the involvement of new enzymes in the early detoxification response of Phanerochaete chrysosporium

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