Reduced aleurone  -amylase production in aged wheat seeds is accompanied by lower levels of high-pI  -amylase transcripts and reduced response to gibberellic acid

Bernal-Lugo, Irma; Rodriguez, Mireya; Gavilanes‐Ruíz, Marina; Hamabata, Alberto

doi:10.1093/jxb/50.332.311

Cited by 5 publications

(5 citation statements)

References 23 publications

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“…Without starch, rT would be too low to approximate real seed data. When the virtual seed is not allowed to produce LMW sugars from starch degradation at later stages of germination and after completion of germination, the simulated profile approximates the measured curve of nonviable seeds in which rT rose rapidly after rT min , consistent with reduced activity of starch-degrading enzymes reported for aged seeds (e.g., wheat) (19).…”

Section: Resultssupporting

confidence: 72%

Noninvasive diagnosis of seed viability using infrared thermography

Kranner

Kastberger²,

Hartbauer³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Recent advances in the noninvasive analyses of plant metabolism include stress imaging techniques, mainly developed for vegetative tissues. We explored if infrared thermography can be used to predict whether a quiescent seed will germinate or die upon water uptake. Thermal profiles of viable, aged, and dead Pisum sativum seeds were recorded, and image analysis of 22,000 images per individual seed showed that infrared thermography can detect imbibition-and germination-associated biophysical and biochemical changes. These "thermal fingerprints" vary with viability in this species and in Triticum aestivum and Brassica napus seeds. Thermogenesis of the small individual B. napus seeds was at the limit of the technology. We developed a computer model of "virtual pea seeds," that uses Monte Carlo simulation, based on the heat production of major seed storage compounds to unravel physico-chemical processes of thermogenesis. The simulation suggests that the cooling that dominates the early thermal profiles results from the dissolution of low molecular-weight carbohydrates. Moreover, the kinetics of the production of such "cooling" compounds over the following 100 h is dependent on seed viability. We also developed a deterministic tool that predicts in the first 3 hours of water uptake, when seeds can be redried and stored again, whether or not a pea seed will germinate. We believe that the early separation of individual, ungerminated seeds (live, aged, or dead) before destructive germination assessment creates unique opportunities for integrative studies on cell death, differentiation, and development.aging | crop | germination | imaging | stress S eeds are attractive experimental model systems to study general biological phenomena, such as aging, cell death, and development. Desiccation tolerant "orthodox" seeds can be stored long term in the dry state, but lethal damage can be induced rapidly by "artificial aging," involving the elevation of seed moisture content (MC) and temperature (1). However, the biochemical and molecular interpretation of aging is hindered by the use of inseparable populations of viable and nonviable seeds, in which the partitioning of analytes in seeds of differential quality is unknown. Seed-quality studies would benefit from a tool that identifies individual viable and nonviable seeds before use. Moreover, global agriculture is fundamentally dependent on the production, distribution, and germination of high-quality seeds.Pioneering studies (2-7) using microcalorimetry (8, 9) demonstrated that metabolic heat flows can be used to assess gross metabolism associated with germination processes. However, microcalorimeters do not capture thermal activity in the first phase of seed imbibition while samples equilibrate in the instrument. In addition, they are closed systems, preventing dissipation of heat and gas, with potential confounding feedback on seed metabolism. Consequently, these microcalorimetric studies were inconclusive as to whether temperature rises or falls during the initial stages of s...

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Section: Resultssupporting

confidence: 72%

Noninvasive diagnosis of seed viability using infrared thermography

Kranner

Kastberger²,

Hartbauer³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…The need to produce cereal-based products all year round means that grains such as wheat can be stored for some time before processing (Arumugam, 2012). During prolonged storage, there is a progressive reduction in the rate of germination and seedling growth, before seed death takes place (Bernal-Lugo et al, 1999). The aging process causes changes that improves the characteristics of the wheat kernels and, hence, enhances their utilization; age-related changes have great influence on viscosity of the batter and the water-binding ability of the wheat flour (Arumugam, 2012).…”

Section: Discussionmentioning

confidence: 99%

Microbial dynamics in durum wheat kernels during aging

Gaglio

Cirlincione

Miceli

et al. 2020

International Journal of Food Microbiology

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“…Furthermore, the endosperm content has been found to affect seed longevity in rice (Oryza sativa L.) and wheat (Triticum aestivum;Bernal-Lugo et al 1999;Petruzzelli and Taranto 1989;Zhou et al 2002). Although PIMT has been shown to exist in monocot seeds, no substantive evidence linking its activity to seed vigor and viability in the seeds from this class has yet been presented.…”

Section: Introductionmentioning

confidence: 99%

Protein repair l -isoaspartyl methyltransferase 1 (PIMT1) in rice improves seed longevity by preserving embryo vigor and viability

Wei

Diao

et al. 2015

Plant Mol Biol

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Damaged proteins containing abnormal isoaspartyl (isoAsp) accumulate as seeds age and the abnormality is thought to undermine seed vigor. Protein-L-isoaspartyl methyltransferase (PIMT) is involved in isoAsp-containing protein repair. Two PIMT genes from rice (Oryza sativa L.), designated as OsPIMT1 and OsPIMT2, were isolated and investigated for their roles. The results indicated that OsPIMT2 was mainly present in green tissues, but OsPIMT1 largely accumulated in embryos. Confocal visualization of the transient expression of OsPIMTs showed that OsPIMT2 was localized in the chloroplast and nucleus, whereas OsPIMT1 was predominately found in the cytosol. Artificial aging results highlighted the sensitivity of the seeds of OsPIMT1 mutant line when subjected to accelerated aging. Overexpression of OsPIMT1 in transgenic seeds reduced the accumulation of isoAsp-containing protein in embryos, and increased embryo viability. The germination percentage of transgenic seeds overexpressing OsPIMT1 increased 9-15% compared to the WT seeds after 21-day of artificial aging, whereas seeds from the OsPIMT1 RNAi lines overaccumulated isoAsp in embryos and experienced rapid loss of seed germinability. Taken together, these data strongly indicated that OsPIMT1-related seed longevity improvement is probably due to the repair of detrimental isoAsp-containing proteins that over accumulate in embryos when subjected to accelerated aging.

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Reduced aleurone -amylase production in aged wheat seeds is accompanied by lower levels of high-pI -amylase transcripts and reduced response to gibberellic acid

Cited by 5 publications

References 23 publications

Noninvasive diagnosis of seed viability using infrared thermography

Noninvasive diagnosis of seed viability using infrared thermography

Microbial dynamics in durum wheat kernels during aging

Protein repair l -isoaspartyl methyltransferase 1 (PIMT1) in rice improves seed longevity by preserving embryo vigor and viability

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