Redox Targeting of Oligonucleotides Anchored to Nanocrystalline TiO2 Films for DNA Detection

Interfacial electron transfer on cytochrome-c sensitised conformally coated mesoporous TiO2 films

“…We have characterized the properties of such protein/TiO 2 electrodes by cyclic voltammetry and UV-visible spectroscopy and demonstrated that the immobilized proteins can be reduced by the application of an electrical potential to the film without the addition of any electron-transfer mediators [1][2][3][4][5]. Moreover, other groups have also shown the adsorption of a range of biomolecules on mesoporous metal oxide electrodes as working electrodes for sensing devices [6][7][8][9][10][11][12][13][14][15].…”

Fabrication of Mesoporous Hollow TiO2Microcapsules for Application as a DNA Separator

“…Nucleotides bind firmly to TiO 2 by complexation of their phosphate groups to the exposed titanium ions. 6 On the other hand, these mesoporous materials may offer the additional advantages, such as the size selectivity with respect to the target DNA by controlling the mesopore size. The electrostatic interactions between the TiO 2 surfaces and target DNA labeled by cyanine (Cy5) were examined by confocal laser scanning microscopy (CLSM).…”

“…5 Meier and Gratzel used nanocrystalline semiconductor oxides (nanocrystalline TiO 2 films) as substrates for immobilizing DNA oligonucleotides. 6 The oxide material of choice was anatase TiO 2 , to which the nucleotide binds firmly via complexation of the phosphate group to the titanium ions on the matrix surfaces. Electron transfer from the conduction bands of the semiconductor oxides to adsorbed mononucleotides has been reported.…”

Direct spectroelectrochemistry of peroxidases immobilised on mesoporous metal oxide electrodes: Towards reagentless hydrogen peroxide sensing

“…We have characterized the properties of such protein/TiO 2 electrodes by cyclic voltammetry and UV-visible spectroscopy and demonstrated that the immobilised proteins can be reduced by the application of an electrical potential to the film without the addition of any electron-transfer mediators [14][15][16]. Moreover, we and other groups have also shown the adsorption of a range of biomolecules on mesoporous metal oxide electrodes as working electrodes for sensing devices [17][18][19][20][21][22][23].…”