Redox-tagged peptide for capacitive diagnostic assays

Santos, Alexandre Leme Godoy dos; Piccoli, Julia P.; Santos-Filho, Norival A.; Cilli, Eduardo Maffud; Bueno, Paulo Agenor Alves

doi:10.1016/j.bios.2014.12.059

Cited by 37 publications

(42 citation statements)

References 43 publications

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“…Yet biocomposites synthesis has been reported based on SAM templates . For example, self‐assembly of peptides monolayers on gold surface demonstrated good characteristics as an alternative to those comprising traditional monolayers . In summary, self‐assembly molecular layers are very suitable and desirable in different applications and peptides‐based molecular function constitutes useful and versatile alternative to be exploited in those kinds of applications, as we will demonstrate below.…”

Section: Introductionmentioning

confidence: 88%

“…The peptide surface coverage (

Γ

, mol cm −2 ) was calculated using the equation

Γ = 4 C_{r} k_{B} T / e F

, providing an occupation at the half‐wave potential where

f = 1 / 2

(where the redox capacitance,

C_{r}

, maximizes), [see Eq. below for more details], where

k_{B}

is the Boltzmann constant,

T

is the absolute temperature (293 K), and

e

is the elementary charge . To compare the

Γ

results obtained using the redox capacitance approach, the surface coverage was estimated using an expression derived from classical voltammetry

Γ = Q / n_{e} F A

, where

n_{e}

is the number of electrons in the electrochemical reaction (

n_{e}

= 1, since

{F c}^{+} + e^{-} ⇆ F c

) and

Q

is the charge obtained by the integration peak of the reduction/oxidation process in the CV, discounting the capacitive contribution.…”

Section: Methodsmentioning

confidence: 99%

“…This type of electroactive surface and structure has been shown to be of major importance in achieving reliable electrochemical capacitive biosensors as we reported recently . Latter application comprises development of medical diagnostics devices . Significant is the fact that electroactive monolayers are valuable for the immobilization of antibodies, proteins, biological molecules, enzymes, aptamers, and others receptors on metal surfaces underpinning a variety of electrochemistry applications in a multiplexing manner .…”

Section: Introductionmentioning

confidence: 97%

“…This is an effective methodology to synthesize different functional molecules, offering an easy preparation and purification methodology and thus providing the ability to design reliable molecular functional structures over electrodes within a multitude of structural and physical‐chemistry properties . Additionally the simple and versatile characteristic involving SPPS allows add redox probes into the amino‐acid chains . By designing peptides with self‐assembling characteristics it is possible to achieve competitive and yet superior versatility, for instance, non‐fouling surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…We report herein on the synthesis, by solid phase, of an electrochemical active peptide with self‐assembling capability that can be used as redox‐tagged peptide in electrochemical capacitive biosensoring surface . Basically the peptide‐based self‐assembly monolayer is comprised of a ferrocene‐tagged group whose electrochemistry activity was characterized by traditional and by a newer capacitive‐based methods.…”

Section: Introductionmentioning

confidence: 99%

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The self‐assembly of redox active peptides: Synthesis and electrochemical capacitive behavior

et al. 2016

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The present work reports on the synthesis of a redox-tagged peptide with self-assembling capability aiming applications in electrochemically active capacitive surfaces (associated with the presence of the redox centers) generally useful in electroanalytical applications. Peptide containing ferrocene (fc) molecular (redox) group (Ac-Cys-Ile-Ile-Lys(fc)-Ile-Ile-COOH) was thus synthesized by solid phase peptide synthesis (SPPS). To obtain the electrochemically active capacitive interface, the side chain of the cysteine was covalently bound to the gold electrode (sulfur group) and the side chain of Lys was used to attach the ferrocene in the peptide chain. After obtaining the purified redox-tagged peptide, the self-assembly and redox capability was characterized by cyclic voltammetry (CV) and electrochemical impedance-based capacitance spectroscopy techniques. The obtained results confirmed that the redox-tagged peptide was successfully attached by forming an electroactive self-assembled monolayer onto gold electrode. The design of redox active self-assembly ferrocene-tagged peptide is predictably useful in the development of biosensor devices precisely to detect, in a label-free platform, those biomarkers of clinical relevance. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 357-367, 2016.

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Section: Introductionmentioning

confidence: 88%

“…The peptide surface coverage (

Γ

, mol cm −2 ) was calculated using the equation

Γ = 4 C_{r} k_{B} T / e F

, providing an occupation at the half‐wave potential where

f = 1 / 2

(where the redox capacitance,

C_{r}

, maximizes), [see Eq. below for more details], where

k_{B}

is the Boltzmann constant,

T

is the absolute temperature (293 K), and

e

is the elementary charge . To compare the

Γ

results obtained using the redox capacitance approach, the surface coverage was estimated using an expression derived from classical voltammetry

Γ = Q / n_{e} F A

, where

n_{e}

is the number of electrons in the electrochemical reaction (

n_{e}

= 1, since

{F c}^{+} + e^{-} ⇆ F c

) and

Q

is the charge obtained by the integration peak of the reduction/oxidation process in the CV, discounting the capacitive contribution.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The self‐assembly of redox active peptides: Synthesis and electrochemical capacitive behavior

et al. 2016

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ArtinM Binding Effinities and Kinetic Interaction with Leukemia Cells: A Quartz Crystal Microbalance Bioelectroanalysis on the Cytotoxic Effect

et al. 2017

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ArtinM, a D‐mannose‐binding lectin from Artocarpus heterophyllus (jackfruit), interacts with N‐glycosylated receptors on the surface of several cells of hematopoietic origin, triggering cell migration, degranulation, and cytokine release. Because malignant transformation is often associated with altered expression of cell surface glycans, we evaluated the interaction of ArtinM with human myelocytic leukemia cells by Quartz Cristal Microbalance (QCM) and cross compared this investigation with cellular responses (cytotoxicity) to lectin binding by the colorimetric assay MTT, which is a standard method in biology. QCM analysis was able to provide additional information (not possible to be obtained by MTT) that impact on the knowledge of medical biology related to leukemic cells. For instance, it was shown that association rate constant of ArtinM and cellular membranes (obtained from QCM) of leukemia cell varies across the myeloid leukemia cell lines, decreasing as cytotoxic effect increases. Meanwhile no differences were observed for dissociation rate constants so that the equilibrium binding affinity constant was observed to be a direct function of the association rate event. It was supposed that ArtinM cytotoxicity is affected by the association time with glycans on the cellular membranes of leukemia cells in a way that the higher it is the association time (the lower was the association rate constant) the higher is the ArtinM cytotoxicity over the leukemia cell lines.

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Introduction to Fundamental Concepts

Bueno

2018

Nanoscale Electrochemistry of Molecular Contacts

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Redox-tagged peptide for capacitive diagnostic assays

Cited by 37 publications

References 43 publications

The self‐assembly of redox active peptides: Synthesis and electrochemical capacitive behavior

The self‐assembly of redox active peptides: Synthesis and electrochemical capacitive behavior

ArtinM Binding Effinities and Kinetic Interaction with Leukemia Cells: A Quartz Crystal Microbalance Bioelectroanalysis on the Cytotoxic Effect

Introduction to Fundamental Concepts

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