Redox Regulation of psbA Gene Expression in Synechococcus SP. PCC 7942

Sippola, Katja; Aro, Eva‐Mari

doi:10.1007/978-94-011-3953-3_681

Cited by 12 publications

(21 citation statements)

References 8 publications

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“…7A). As previously reported in Synechocystis, psbAII transcript abundance increased substantially in cells treated with DTT red and DCMU (Sippola and Aro, 1999;Li and Sherman, 2000). GLY treatment, which would be expected to emulate a high-light stress due to over-reduction of the photosynthetic electron transport chain, also resulted in an increase in psbAII abundance.…”

Section: Effect Of Redox Modifiers and Inhibitors Of Co 2 Fixation Orsupporting

confidence: 60%

“…Direct sensing of external or internal Ci availability, changes in the redox potential of the photosynthetic electron transport chain or in the amounts of intermediates in the Calvin cycle, and photorespiratory pathways have all been proposed (for review, see Kaplan and Reinhold, 1999). It is well established that light-regulated expression of the psbA family occurs through a redox-sensing mechanism in cyanobacterial cells (Sippola and Aro, 1999;Alfonso et al, 2000;Li and Sherman, 2000;van Waasbergen et al, 2002). Given the finding that the expression of members of the psbA and pbsD families is responsive to Ci availability and reports that some CCM-related gene expression is light-responsive (Hihara et al, 2001;Huang et al, 2002), a redox-sensing mechanism modulating CCM-related gene expression seems to be an attractive theory.…”

Section: Discussionmentioning

confidence: 99%

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Inorganic Carbon Limitation Induces Transcripts Encoding Components of the CO2-Concentrating Mechanism in Synechococcus sp. PCC7942 through a Redox-Independent Pathway

2003

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The cyanobacterial CO 2-concentrating mechanism (CCM) allows photosynthesis to proceed in CO 2-limited aquatic environments , and its activity is modulated in response to inorganic carbon (Ci) availability. Real-time reverse transcriptase-PCR analysis was used to examine the transcriptional regulation of more than 30 CCM-related genes in Synechococcus sp. strain PCC7942 with an emphasis on genes encoding high-affinity Ci transporters and carboxysome-associated proteins. This approach was also used to test hypotheses about sensing of Ci limitation in cyanobacteria. The transcriptional response of Synechococcus sp. to severe Ci limitation occurs rapidly, being maximal within 30 to 60 min, and three distinct temporal responses were detected: (a) a rapid, transient induction for genes encoding carboxysome-associated proteins (ccmKLMNO, rbcLS, and icfA) and the transcriptional regulator, cmpR; (b) a slow sustained induction of psbAII; and (c) a rapid sustained induction of genes encoding the inducible Ci transporters cmpABCD, sbtA, and ndhF3-D3-chpY. The Ci-responsive transcripts investigated had half-lives of 15 min or less and were equally stable at high and low Ci. Through the use of a range of physiological conditions (light and Ci levels) and inhibitors such as 3-(3,4-dichlorophenyl)-1,1dimethylurea, glycolaldehyde, dithiothreitol, and ethoxyzolamide, we found that no strict correlation exists between expression of genes known to be induced under redox stress, such as psbAII, and the expression of the Ci-responsive CCM genes. We argue that redox stress, such as that which occurs under highlight stress, is unlikely to be a primary signal for sensing of Ci limitation in cyanobacteria. We discuss the data in relation to current theories of CO 2 sensing in cyanobacteria.

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Section: Effect Of Redox Modifiers and Inhibitors Of Co 2 Fixation Orsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Inorganic Carbon Limitation Induces Transcripts Encoding Components of the CO2-Concentrating Mechanism in Synechococcus sp. PCC7942 through a Redox-Independent Pathway

2003

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“…D1:1 is expressed during normal growth conditions at low or moderate light intensities (17). Upon exposure to high light (17)(18)(19), UV-B (20), chilling stress (21), or reductive stress (22), D1:2 is up-regulated and replaces D1:1 in PSII reaction centers. Algae and higher plants contain only one D1 isoform per species.…”

Section: Photosystem II (Psii)mentioning

confidence: 99%

Natural Variants of Photosystem II Subunit D1 Tune Photochemical Fitness to Solar Intensity *

Vinyard

Gimpel

Ananyev

et al. 2013

Journal of Biological Chemistry

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Background: Cyanobacteria use multiple PSII-D1 isoforms to adapt to environmental conditions. Results: D1:2 achieves higher quantum efficiency of water oxidation and biomass accumulation rate at high light versus D1:1; the latter is more efficient at low light due to less charge recombination. Conclusion: A functional advantage for D1:1 is revealed for the first time. Significance: Improved photochemical efficiency at low light suggests an evolutionary advantage to retain D1:1.

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“…However, redox regulation of cyanobacterial gene expression is not restricted to the PQ pool alone. Additional redox signals come from PSII excitation pressure and the thiol state of the cell [51,52]. Furthermore, as well as the reaction-centre genes, several other genes are also regulated by signals from electron transport, generated either photosynthetically or from glucose metabolism.…”

Section: Trends In Plantmentioning

confidence: 99%