Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana

Díaz, Manuel Guinea; Hernández‐Verdeja, Tamara; Kremnev, Dmitry; Crawford, Tim S.; Dubreuil, Carole; Strand, Åsa

doi:10.1038/s41467-017-02468-2

Cited by 47 publications

(41 citation statements)

References 62 publications

(83 reference statements)

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“…RNA was quantified using the NanoDrop spectrophotometer (Thermo Scientific). cDNA synthesis and real-time PCR performed as described by Díaz et al 58 . Gene-specific primers were designed with Primer3 59 and listed in Supplementary Table 1.…”

Section: Methodsmentioning

confidence: 99%

Two dominant boreal conifers use contrasting mechanisms to reactivate photosynthesis in the spring

et al. 2020

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Boreal forests are dominated by evergreen conifers that show strongly regulated seasonal photosynthetic activity. Understanding the mechanisms behind seasonal modulation of photosynthesis is crucial for predicting how these forests will respond to changes in seasonal patterns and how this will affect their role in the terrestrial carbon cycle. We demonstrate that the two co-occurring dominant boreal conifers, Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies), use contrasting mechanisms to reactivate photosynthesis in the spring. Scots pine downregulates its capacity for CO 2 assimilation during winter and activates alternative electron sinks through accumulation of PGR5 and PGRL1 during early spring until the capacity for CO 2 assimilation is recovered. In contrast, Norway spruce lacks this ability to actively switch between different electron sinks over the year and as a consequence suffers severe photooxidative damage during the critical spring period.

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Section: Methodsmentioning

confidence: 99%

Two dominant boreal conifers use contrasting mechanisms to reactivate photosynthesis in the spring

et al. 2020

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“…Biochemical studies enabled functional specificity to be assigned to different plastidial TRXs, leading to a global picture in which the f and m-type TRXs are regulators of enzymes directly or indirectly linked to photosynthetic carbon metabolism, while the x and the y types appear to have antioxidant functions [4,5,6,7]. TRX z which displays unique properties among plastidial TRXs [8,9] has been recently validated as a regulator of plastidial gene expression [10]. Recent studies, using Trx mutant plants and over-expressors, allowed some of these functions to be confirmed in planta , enabling evaluation of the degree of redundancy between the various TRX types.…”

Section: Introductionmentioning

confidence: 99%

Redox Regulation of Monodehydroascorbate Reductase by Thioredoxin y in Plastids Revealed in the Context of Water Stress

et al. 2018

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Thioredoxins (TRXs) are key players within the complex response network of plants to environmental constraints. Here, the physiological implication of the plastidial y-type TRXs in Arabidopsis drought tolerance was examined. We previously showed that TRXs y1 and y2 have antioxidant functions, and here, the corresponding single and double mutant plants were studied in the context of water deprivation. TRX y mutant plants showed reduced stress tolerance in comparison with wild-type (WT) plants that correlated with an increase in their global protein oxidation levels. Furthermore, at the level of the main antioxidant metabolites, while glutathione pool size and redox state were similarly affected by drought stress in WT and trxy1y2 plants, ascorbate (AsA) became more quickly and strongly oxidized in mutant leaves. Monodehydroascorbate (MDA) is the primary product of AsA oxidation and NAD(P)H-MDA reductase (MDHAR) ensures its reduction. We found that the extractable leaf NADPH-dependent MDHAR activity was strongly activated by TRX y2. Moreover, activity of recombinant plastid Arabidopsis MDHAR isoform (MDHAR6) was specifically increased by reduced TRX y, and not by other plastidial TRXs. Overall, these results reveal a new function for y-type TRXs and highlight their role as major antioxidants in plastids and their importance in plant stress tolerance.

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“…Distinct from the other GUN genes, GUN1 is the only known GUN gene that, when defective, results in derepression of the expression of PhANGs in the presence of both Lin (which inhibits PGE by blocking chloroplast translation) and NF (which affects TPB signaling; Koussevitzky et al, 2007;Wu et al, 2018Wu et al, , 2019. Interestingly, the GUN1 mutation was shown to restore PhANG expression in plastid sigma factor mutants (Woodson et al, 2013), the prors1 mutant (defective in prolyl-tRNA synthetase in both chloroplasts and mitochondria; Tadini et al, 2016), and the prin2 mutant (affecting a locus required for full expression of genes transcribed by the plastid-encoded RNA polymerase; Kindgren et al, 2012a;Díaz et al, 2018). Together, these findings indicate a central role of GUN1 in the PGE pathway of retrograde signaling.…”

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confidence: 99%

Extensive Posttranscriptional Regulation of Nuclear Gene Expression by Plastid Retrograde Signals

Meyer

Bock

2019

Plant Physiol.

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Retrograde signals emanate from the DNA-containing cell organelles (plastids and mitochondria) and control the expression of a large number of nuclear genes in response to environmental and developmental cues. Previous studies on retrograde signaling have mainly analyzed the regulation of nuclear gene expression at the transcript level. To determine the contribution of translational and posttranslational regulation to plastid retrograde signaling, we combined label-free proteomics with transcriptomic analysis of Arabidopsis (Arabidopsis thaliana) seedlings and studied their response to interference with the plastid gene expression pathway of retrograde signaling. By comparing the proteomes of the genomes uncoupled1 (gun1) and gun5 mutants with the wild type, we show that GUN1 is critical in the maintenance of plastid protein homeostasis (proteostasis) when plastid translation is blocked. Combining transcriptomic and proteomic analyses of the wild type and gun1, we identified 181 highly translationally or posttranslationally regulated (HiToP) genes. We demonstrate that HiToP photosynthesis-associated nuclear genes (PhANGs) are largely regulated by translational repression, while HiToP ribosomal protein genes are regulated posttranslationally, likely at the level of protein stability without the involvement of GUN1. Our findings suggest distinct posttranscriptional control mechanisms of nuclear gene expression in response to plastid-derived retrograde signals. They also reveal a role for GUN1 in the translational regulation of several PhANGs and highlight extensive posttranslational regulation that does not necessitate GUN1. This study advances our understanding of the molecular mechanisms underlying intracellular communication and provides new insight into cellular responses to impaired plastid protein biosynthesis.

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Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana

Cited by 47 publications

References 62 publications

Two dominant boreal conifers use contrasting mechanisms to reactivate photosynthesis in the spring

Two dominant boreal conifers use contrasting mechanisms to reactivate photosynthesis in the spring

Redox Regulation of Monodehydroascorbate Reductase by Thioredoxin y in Plastids Revealed in the Context of Water Stress

Extensive Posttranscriptional Regulation of Nuclear Gene Expression by Plastid Retrograde Signals

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