Redox proteomics in the mussel, Mytilus edulis

Mussel histopathology: effects of season, disease and species

“…Core methods include scope for growth, histopathology, metallothionein concentration, lysosomal stability and cholinesterase (AChE) inhibition (Widdows & Salkeld 1992, Viarengo et al 1997, Lowe & Fossato 2000, Rickwood & Galloway 2004, Marigómez et al 2006. Additionally, with increasing emphasis on the application of 'omic' technologies in monitoring programmes, mussels once again appear suitably placed for future biomarker discoveries utilising these technologies (Manduzio et al 2005, McDonagh et al 2006, Hines et al 2007a. In light of their increased usage as marine sentinels, it is important to consider the impact of our findings on the future application of biomarker technologies to field-collected animals and to provide recommendations for the collection of health parameter and species data to coincide with other biological and chemical measures.…”

2-D difference gel electrophoresis approach to assess protein expression profiles in Bathymodiolus azoricus from Mid-Atlantic Ridge hydrothermal vents

“…Recent proteomic studies with other bivalve species also show that actin is a target protein for contaminants in the clam Ruditapes decussatus exposed to Cd and nonylphenol [29,65], in the mussel Mytilus edulis [34]. Other proteomic studies working with redox modification of proteins in M. edulis from polluted and clean sites clearly imply a protective role of actin against oxidative stress [66]. Three other differently expressed proteins identified in B. azoricus were metabolic proteins with high homology with a putative ubiquinone biosynthesis protein, Sadenosylhomocysteinase hydrolase and cysteine peptidases.…”

The role of proteomics in the study of the influence of climate change on seafood products

“…As an example, high levels of these modifications have been showed by 2-DE and immunoblot in the digestive glands of the marine bivalves (Chora et al, 2008). Metals in aquatic environments can generate the formation of reactive oxygen species (ROS), producing effects as carbonylation and ubiquitination of proteins (McDonagh, Tyther, & Sheehan, 2006). Specific staining methods to detect carbonyl groups in proteins separated by 2-DE have been developed (Talent, Kong, & Gracy, 1998) .…”

“…Not proteomics Pörtner, Langenbuch, and Michaelidis 2005 Myoglobin expression levels influenced by hypoxia in carp. (microarrays) Fraser et al (2006) Oxidative proteomic stress and HSP in clams Dowling et al (2006) Oxidative stress in mussels McDonagh and Sheehan (2007) Redox proteomics in mussel and McDonagh, Tyther, and Sheehan (2006) Ubiquitination and carbonylation as oxidative stress indicators in clams Chora et al (2008) Proteomic of mud crab gill under low temperature adaptation Wang, MacKenzie, et al (2007) Environmental toxicology Zebra fish model in assessment Scholz et al (2008) Mussel as sentinel in pollution Zorita et al (2007) Heavy metals and stress effects in Atlantic salmon Salbu et al (2008) Goldfish liver adaptation to environmental stress Wang, Wei, Wang, Chan, and Dai (2008) Rainbow trout proteomic endocrine disruptors characterization as biomarkers Smith, Salaberria, Cash, and Pärt (2007) Pollutant responses in marine organisms (PRIMO 13) Symposium Abstracts Viarengo, 2006 Pollutant responses in marine organisms (PRIMO 14) Symposium Abstracts Bainy (2008) Marine pollution and proteomics Amelina, Apraiz, Sun, and Cristobal (2007) and Cristobal (2008) Proteomics signatures of pollution in mussel Apraiz, Mi, and Cristobal (2006) allergen proteins have been characterized in fishing products (Permyakov, Karnoup, Bakunts, & Permyakov, 2009), as it has been done with specific peptide sequences indicative of parvalbumin presence in hake manufactured products (Carrera, Cañas, Piñeiro, Vá zquez, & Gallardo, 2006). Shellfish allergy has been studied (Motoyama, Suma, Ishizaki, Nagashima, & Shiomi 2007) and some allergens have been included in the data base using proteomic strategies: arginine kinase from Penaeus monodon (Yu, Lin, Chiang, & Chow, 2003), the black tiger shrimp, and myosin light chain from the white leg Pacific shrimp (Litopenaeus vannamei) (Ayuso et al, 2008) and several commercial specific ELISA tests have been developed using those prawn proteins.…”