Redox properties ofAbarema cochliacarpos(Gomes) Barneby & Grime (Fabaceae) stem bark ethanol extract and fractions

Abstract

The redox properties of the hydroethanol extract (EE) and its ethyl acetate (EAF) and hydromethanol (HMF) fractions obtained from Abarema cochliacarpos (Gomes) Barneby & Grimes stem bark were evaluated. EAF had the highest total phenol content (848.62 ± 78.18 mg g⁻¹), while EE showed the highest content of catechin (71.2 µg g⁻¹). EE, EAF and HMF exhibited the highest levels of antioxidant activity at 100 and 1000 µg mL⁻¹ when the non-enzymatic antioxidant potential was evaluated by the total reactive antioxidant potential, total antioxidant reactivity and nitric oxide scavenging assays. In addition, EAF and HMF showed SOD-like activity. The results for EE, EAF and HMF in this study showed that A. cochliacarpos (Gomes) Barneby & Grimes stem bark have redox properties and may be able to help the endogenous enzymatic and non-enzymatic systems to keep the redox balance.

Abarema cochliacarposExtract Decreases the Inflammatory Process and Skeletal Muscle Injury Induced byBothrops leucurusVenom

“…In agreement with previous studies [11, 27], the anti-inflammatory properties of dexamethasone were able to partially inhibit the edema induced by venom injection, strongly supporting that at least in part the venom activity depends on inflammation. Interestingly, the crude extract of A. cochliacarpos was also able to reduce the edematogenic effect of B. leucurus venom injection, confirming early observations showing that this plant presents some anti-inflammatory properties [16, 28–31]. …”

“…Other authors also observed similar applications in different traditional communities [14, 15]. According to previous studies, the hydroethanolic extract presents phenolics such as aurones, catechins, chalcones, flavanols, flavones, flavonols, leucoanthocyanidins, tannins and xanthones, besides saponins, and steroids [16]. …”

“…Several groups have demonstrated potential therapeutic uses of A. cochliacarpos , such as protective effects in acute experimental colitis [28], gastroprotective effects and wound-healing properties [29], and antioxidant and anti-inflammatory properties [16, 31]. Those abilities have been related to inhibition of oxidative stress and inflammatory responses via direct downregulation of nitric oxide (NO) and reactive oxygen species (ROS) generation, which could damage different cellular components such as lipids, proteins, and DNA.…”

“…Those abilities have been related to inhibition of oxidative stress and inflammatory responses via direct downregulation of nitric oxide (NO) and reactive oxygen species (ROS) generation, which could damage different cellular components such as lipids, proteins, and DNA. The antioxidant and anti-inflammatory properties of A. cochliacarpos hydroethanolic extract may be mediated, at least in part, by the presence of (+)-catechin, a flavonoid-type compound, the most abundant in the extract, which induce downregulation of proinflammatory cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) enzymes [16, 28, 29, 31]. …”

“…After this period, the extract was filtered and concentrated in a rotary evaporator under reduced pressure at 50°C yielding 533.4 g of hydroethanolic extract. The phytochemical analysis of A. cochliacarpos has been recently performed and described [16]. …”