Redox Modification of the Iron-Sulfur Glutaredoxin GRXS17 Activates Holdase Activity and Protects Plants from Heat Stress

Martins, Laura; Knuesting, Johannes; Bariat, Laetitia; Dard, Avilien; Freibert, Sven‐Andreas; Marchand, Christophe; Young, Diana; Dung, Nguyen Ho Thuy; Voth, Wilhelm; Debures, Anne; Sáez‐Vasquez, Julio; Lemaire, Stéphane D.; Lill, Roland; Messens, Joris; Scheibe, Renate; Reichheld, Jean‐Philippe; Riondet, Christophe

doi:10.1104/pp.20.00906

Cited by 38 publications

(32 citation statements)

References 75 publications

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“…Though HSPs might be interacting target proteins of GRXs, how AtGRXS17 interacts with HSPs is unknown. In the recent study, GRXS17 is found to possess both foldase and redox-dependent holdase activity which are further supporting the role of GRXS17 as a molecular chaperone [ 70 ].…”

Section: Discussionmentioning

confidence: 74%

Ectopic Expression of a Heterologous Glutaredoxin Enhances Drought Tolerance and Grain Yield in Field Grown Maize

Tamang

Sprague

Kakeshpour

et al. 2021

IJMS

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Drought stress is a major constraint in global maize production, causing almost 30–90% of the yield loss depending upon growth stage and the degree and duration of the stress. Here, we report that ectopic expression of Arabidopsis glutaredoxin S17 (AtGRXS17) in field grown maize conferred tolerance to drought stress during the reproductive stage, which is the most drought sensitive stage for seed set and, consequently, grain yield. AtGRXS17-expressing maize lines displayed higher seed set in the field, resulting in 2-fold and 1.5-fold increase in yield in comparison to the non-transgenic plants when challenged with drought stress at the tasseling and silking/pollination stages, respectively. AtGRXS17-expressing lines showed higher relative water content, higher chlorophyll content, and less hydrogen peroxide accumulation than wild-type (WT) control plants under drought conditions. AtGRXS17-expressing lines also exhibited at least 2-fold more pollen germination than WT plants under drought stress. Compared to the transgenic maize, WT controls accumulated higher amount of proline, indicating that WT plants were more stressed over the same period. The results present a robust and simple strategy for meeting rising yield demands in maize under water limiting conditions.

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Section: Discussionmentioning

confidence: 74%

Ectopic Expression of a Heterologous Glutaredoxin Enhances Drought Tolerance and Grain Yield in Field Grown Maize

Tamang

Sprague

Kakeshpour

et al. 2021

IJMS

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“…The chaperone activity of APX is not an isolated example for an antioxidant enzyme. Upon destabilization of the iron-sulfur cluster in a redox-and temperature-dependent manner, GRXS17 becomes a holdase whose activity probably protects the root meristem under high temperatures (Martins et al, 2020), and possibly could be linked also to its rerouting to the nucleus, because a GFP-GRXS17 fusion protein resides in the nucleus during heat stress (Wu et al, 2012). Moreover, overexpression of AtGRXS17 in tomato improved heat stress resilience together with improved redox homeostasis and elevated HSP expression (Wu et al, 2012).…”

Section: Beating the Heat: Keeping The Antioxidant Machinery At Speedmentioning

confidence: 99%

Improving oxidative stress resilience in plants

Kerchev

Breusegem

2021

The Plant Journal

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Originally conceived as harmful metabolic byproducts, reactive oxygen species (ROS) are now recognized as an integral part of numerous cellular programs. Thanks to their diverse physicochemical properties, compartmentalized production, and tight control exerted by the antioxidant machinery they activate signaling pathways that govern plant growth, development, and defense. Excessive ROS levels are often driven by adverse changes in environmental conditions, ultimately causing oxidative stress. The associated negative impact on cellular constituents have been a major focus of decade-long research efforts to improve the oxidative stress resilience by boosting the antioxidant machinery in model and crop species. We highlight the role of enzymatic and non-enzymatic antioxidants as integral factors of multiple signaling cascades beyond their mere function to prevent oxidative damage under adverse abiotic stress conditions.

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“…However, an impaired thermotolerance of the grxS17 was previously reported (Cheng et al, 2011) (Knuesting et al, 2015) (Martins et al, 2020a). While at 27°C, the grxS17 mutation was suggested to impair auxin signaling (Cheng et al, 2011), the 35°C treatment was also suggested to induce holdase activities of GRXS17 (Martins et al, 2020a). Whether a lower glutathione level would limit GRXS17 thermotolerance activities should be further investigated.…”

Section: Discussionmentioning

confidence: 93%

“…This might be due to redundancy between members of the large glutaredoxins family (Riondet et al, 2012). However, an impaired thermotolerance of the grxS17 was previously reported (Cheng et al, 2011;Knuesting et al, 2015;Martins et al, 2020a). While at 27°C, the grxS17 mutation was suggested to impair auxin signaling (Cheng et al, 2011), the 35°C treatment was also suggested to induce holdase activities of GRXS17 (Martins et al, 2020a).…”

Section: Discussionmentioning

confidence: 95%

Glutathione-mediated plant response to high-temperature

Dard

Bariat

Weiss

et al. 2022

Preprint

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In the context of the climate change, global rise of temperature as well as intense heat waves affect plant development and productivity. Among the molecular perturbations that high temperature induces in living cells is the accumulation of reactive oxygen species (ROS), which can damage macromolecules of the cell and perturb the cellular redox state. To cope with deleterious effects of ROS, plant, as other organisms, have developed strategies to scavenge ROS and to regulate their redox state. Among those, glutathione plays a major role in maintaining the cellular redox state and the function of key antioxidant enzymes like peroxidases. Here, we investigated the contribution of the redox systems in plant adaptation to high temperature. We studied two different high temperature regimes: a rise of ambient temperature to 27C inducing a plant developmental adaptation program called thermomorphogenesis, and a 37C treatment mimicking intense heat wave and affecting plant viability. Using the genetically encoded redox marker roGFP, we show that high temperature regimes lead to cytoplasm and nuclear oxidation and impact profoundly the glutathione pool rather than the glutathione redox state. Moreover, plant can restore the pool within a few hours, which likely contribute to plant adaptation to high temperature. However, conditional glutathione deficient mutants fail to adapt to intense heat waves or to induce thermomorphogenesis, suggesting that glutathione is involved in both heat adaptation mechanisms. We also evaluate by RNAseq analyses, how plant change its genome expression signature upon heat stress and identified a marked genome expression deviation in the glutathione deficient mutant which might contribute to their sensitivity to high temperature. Thus, we define glutathione as a major actor in the adaptation of plant to contrasting high temperature regimes.

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Redox Modification of the Iron-Sulfur Glutaredoxin GRXS17 Activates Holdase Activity and Protects Plants from Heat Stress

Abstract: A cysteine-dependent redox modification of the iron-sulfur cluster glutaredoxin GRXS17 activates its holdase activity and protects plants from heat stress AUTHOR CONTRIBUTION

Cited by 38 publications

References 75 publications

Ectopic Expression of a Heterologous Glutaredoxin Enhances Drought Tolerance and Grain Yield in Field Grown Maize

Ectopic Expression of a Heterologous Glutaredoxin Enhances Drought Tolerance and Grain Yield in Field Grown Maize

Improving oxidative stress resilience in plants

Glutathione-mediated plant response to high-temperature

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