Redox imbalance influence in the myocardial Akt activation in aged rats treated with DHEA

Dehydroepiandrosterone improves hepatic antioxidant reserve and stimulates Akt signaling in young and old rats

“…These results are completely different from myocardial analysis [24], in which DHEA augmented the GSSG levels in the aged group when compared to its control, and also when compared to 3-month-old treated group. The overall data reinforce that DHEA treatment may provide distinct answers depending on tissue and its susceptibility to the redox imbalance.…”

“…These changes would be related to DHEA treatment once its effects over oxidative stress have been largely described in literature [16,24,32,37,39,40]. Mastrocola et al [37] demonstrated the dual effect of DHEA in the liver, depending on the dosage.…”

“…Control groups received vehicle (vegetal oil). DHEA protocol administration was based on other studies [16,21,23,24]. Animals were housed in plastic cages (four animals in each) and received water and pelleted food ad libitum.…”

Aging might augment reactive oxygen species (ROS) formation and affect reactive nitrogen species (RNS) level after myocardial ischemia/reperfusion in both humans and rats

“…·O 2 − further dismutates to H 2 O 2 and ·OH, the latter being highly toxic to biological tissues, causative of significant myocardial necrosis and apoptosis. Several recent studies report that aging increases reactive oxygen species (ROS) (Adler et al 2003;Jacob et al 2010a;Jacob et al 2010b;Judge et al 2005). Leeuwenburgh and colleagues demonstrate increased mitochondrial ROS formation with aging (Judge et al 2005).…”

Oxidative Stress Induction in Monosex Nile Tilapia (Oreochromis niloticus, Linnaeus, 1758): A Field Study on the Side Effects of Methyltestosterone

“…Assessment of mRNA antioxidant enzyme genes expression in human vascular endothelial cells treated with supra-physiological doses of testosterone enanthate provides mechanistic information relating testosterone exposure to oxidative stress initiation [36]. In contrast, dehydroepiandrosterone as an adrenal androgen increased total glutathione content and reduced glutathione in young and old healthy rats [37], and glutathione-S-transferase in aged rats [38]. In addition, testosterone reversed the adverse effects of ovariectomy and 3-nitropropionic acid on reduced glutathione content and glutathione peroxidase activity in striatum of rats [39], increased activities of superoxide dismutase and glutathione peroxidase in cardiomyocytes of testicular feminized and castrated male mice [40], and total antioxidation capability in mice [41] In vitro, it enhanced catalase activity in undifferentiated mouse neuroblastoma cells [42], and glutathione reductase activity, and increased the content of thiol groups in human neutrophils [43].…”