Redox-Dependent Structural Modification of Nucleoredoxin Triggers Defense Responses against Alternaria brassicicola in Arabidopsis

Kang, Chang Ho; Park, Joung Hun; Lee, Eun Seon; Paeng, Seol Ki; Chae, Ho Byoung; Hong, Jong Chan; Lee, Sang Yeol

doi:10.3390/ijms21239196

Cited by 9 publications

(3 citation statements)

References 64 publications

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“…In chestnut blight pathogen, corn smut fungus Ustilago maydis is found to secrete a pathogenicity-related thioredoxin ( Muller et al, 2008 ). In Alternaria brassicicola , which are the causative agent of brassica dark leaf spot, thioredoxin gene expression has been detected following exposure to defense metabolites ( Kang et al, 2020 ). That is why thioredoxin has been classified as stress, oxidative burst, and defense gene in causal agent of brassica dark leaf spot.…”

Section: Discussionmentioning

confidence: 99%

Secretome Analysis of Macrophomina phaseolina Identifies an Array of Putative Virulence Factors Responsible for Charcoal Rot Disease in Plants

2022

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Macrophomina phaseolina is a global devastating necrotrophic fungal pathogen. It causes charcoal rot disease in more than 500 host plants including major food crops, pulse crops, fiber crops, and oil crops. Despite having the whole-genome sequence of M. phaseolina, understanding the M. phaseolina genome-based plant–pathogen interactions is limited in the absence of direct experimental proof of secretion. Thus, it is essential to understand the host–microbe interaction and the disease pathogenesis, which can ensure global agricultural crop production and security. An in silico–predicted secretome of M. phaseolina is unable to represent the actual secretome. We could identify 117 proteins present in the secretome of M. phaseolina using liquid chromatography–electrospray ionization–tandem mass spectrometry. Data are available via ProteomeXchange with identifier PXD032749. An array of putative virulence factors of M. phaseolina were identified in the present study using solid-state culture. Similar virulence factors have been reported in other plant pathogenic fungi also. Among the secretory fungal proteins with positive economic impacts, lignocellulolytic enzymes are of prime importance. Further, we validated our results by detecting the cell wall–degrading enzymes xylanase, endoglucanase, and amylase in the secretome of M. phaseolina. The present study may provide a better understanding about the necrotrophic fungi M. phaseolina, which modulate the host plant defense barriers using secretory proteins.

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Section: Discussionmentioning

confidence: 99%

Secretome Analysis of Macrophomina phaseolina Identifies an Array of Putative Virulence Factors Responsible for Charcoal Rot Disease in Plants

2022

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“…2), whereas both display low accumulation in leaves. The Atnrx1 mutant plant shows a reduced pollen fertility phenotype [64], whereas the Atnrx2 knockout plant develops less trichomes compared with the wild-type and the Atnrx1 mutant [65,66].…”

Section: Expression Of Trxs and Trs In Arabidopsis Organsmentioning

confidence: 99%

Genome‐wide analysis and transcriptional regulation of the typical and atypical thioredoxins in Arabidopsis thaliana

et al. 2021

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Thioredoxins (TRXs), a large subclass of ubiquitous oxidoreductases, are involved in thiol redox regulation. Here, we performed a comprehensive analysis of TRXs in the Arabidopsis thaliana genome, revealing 41 genes encoding 18 typical and 23 atypical TRXs, and 6 genes encoding thioredoxin reductases (TRs). The high number of atypical TRXs indicates special functions in plants that mostly await elucidation. We identified an atypical class of thioredoxins called TRX-c in the genomes of photosynthetic eukaryotes. Localized to the chloroplast, TRX-c displays atypical CPLC, CHLC and CNLC motifs in the active sites. In silico analysis of the transcriptional regulations of TRXs revealed high expression of TRX-c in leaves and strong regulation under cold, osmotic, salinity and metal ion stresses.

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“…Thioredoxin domain-containing protein 9 (TXndc9; also known as aTP-binding protein associated with cell differentiation or phosducin-like family of proteins 3), belongs to the small, highly-conserved and ubiquitous TrX family which is implicated in multiple biological processes via modulating oxidative stress response (6,7). TXndc9 is upregulated in numerous types of cancer, promoting their development.…”

Section: Introductionmentioning

confidence: 99%

TXNDC9 knockdown inhibits lung adenocarcinoma progression by targeting YWHAG

Wang

Pan

et al. 2022

Mol Med Rep

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lung adenocarcinoma (luad) is the most common form of lung cancer and with the highest mortality rate. Therefore, the identification and development of effective methods for the treatment of luad is of great importance. The present study aimed to investigate the role of thioredoxin domain-containing protein 9 (TXndc9) and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein γ (YWHaG; also known as 14-3-3γ) in the progression of luad. The expression of TXndc9 and its association with the survival of patients with luad was analyzed using encyclopedia of rna interactomes. reverse transcription-quantitative Pcr and western blot analysis were used to detect TXndc9 mrna and protein expression levels, respectively, in in vitro studies. To investigate the role of TXndc9 in the progression of luad, TXndc9 was silenced using small interfering rna transfection. Furthermore, the viability, proliferation, migration, invasiveness and apoptosis of TXndc9-silenced a549 cells were detected using cell counting Kit (ccK)-8, colony formation, wound healing, Transwell and Tunel assays, respectively. The association between TXndc9 and YWHaG was analyzed using STrinG and Gene Expression Profiling Interactive Analysis databases, as well as co-immunoprecipitation assays. Subsequently, YWHaG was overexpressed to similarly determine effects of YWHaG on viability, proliferation, migration, invasiveness and apoptosis of a549 cells. TXndc9 expression was markedly upregulated in lung cancer cells, particularly a549 cells, and silencing of TXndc9 expression suppressed the viability of the lung cancer cells. The results also revealed that TXndc9 silencing exerted inhibitory effects on the viability, proliferation, migration and invasiveness of a549 cells, whereas the apoptotic rate was increased. Similar to TXndc9, YWHaG expression was also upregulated in the a549 cells. Furthermore, TXndc9 was demonstrated to bind to YWHaG and was positively associated with YWHaG. YWHaG overexpression reversed the inhibitory effects of TXndc9 silencing on luad, as evidenced by increased viability, proliferation, migration and invasiveness, and decreased apoptosis, of a549 cells. The present study demonstrated that the knockdown of TXndc9 exerted suppressive effects on luad, whereas YWHaG overexpression reversed the inhibitory effects of TXndc9 silencing on luad. Therefore, TXndc9 silencing may exert protective effects against luad by targeting YWHaG.

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Redox-Dependent Structural Modification of Nucleoredoxin Triggers Defense Responses against Alternaria brassicicola in Arabidopsis

Cited by 9 publications

References 64 publications

Secretome Analysis of Macrophomina phaseolina Identifies an Array of Putative Virulence Factors Responsible for Charcoal Rot Disease in Plants

Secretome Analysis of Macrophomina phaseolina Identifies an Array of Putative Virulence Factors Responsible for Charcoal Rot Disease in Plants

Genome‐wide analysis and transcriptional regulation of the typical and atypical thioredoxins in Arabidopsis thaliana

TXNDC9 knockdown inhibits lung adenocarcinoma progression by targeting YWHAG

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