Redox Denaturation of Proteins: Electrochemical Treatment of Egg Plasma

Taniguchi, Daisuke; Handa, Akihiro; Kodama, Risa; Okada, Yohei; Chiba, Kazuhiro

doi:10.1002/elan.201900057

“…Neither protein aggregation nor RBC clots formed by the use of low-temperature plasma have been reported except in our experimental results [27][28][29]. However, it has recently been reported that the formation of disulfide bonds in food proteins was achieved by an electrochemical reaction in solution [35][36][37]. Studies on protein reactions using electric, photonics, or magnetic fields have also become popular in the field of organic chemistry.…”

Section: Hemolytic Coagulation Of Rbc Serum Protein Aggregation Plate...mentioning

confidence: 66%

Effects of electric charges on serum protein aggregation induced by a low temperature atmospheric pressure plasma

Sakakita¹,

Shimizu²,

Fujiwara³

et al. 2021

J. Phys. D: Appl. Phys.

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To understand the aggregation mechanism of serum protein dispersed in the solution of distilled water induced by a low temperature atmospheric pressure plasma, we compared the relationship between the amount of aggregation and experimental condition dependencies such as gas species, gas flow rate, and the distance between the plasma device and the solution. In this experiment, pure argon, helium, and/or a mixture of helium and argon gases were used. From statistical analyses of various experimental conditions, it was found that a monotonic supply of total absolute charge in one period was important for protein aggregation. When the coefficient of variation (standard deviations/averaged total absolute charges during one period) is small, the amount of protein aggregation becomes larger. Excess or scarcity of averaged total absolute charges does not appear to relate to protein aggregation phenomena; however, the coefficient of variation strongly relates to the mechanism of protein aggregation. Therefore, a possible serum protein aggregation process by low-temperature plasma treatment was proposed in which the serum protein, which had negative charges in the solution, was locally aggregated through the trigger of charge neutralization induced by a stable charge supply, and/or positively charged aggregated protein on the solution surface attracted serum protein in the solution to the surface.

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“…Noticed that, it is universally known that proteins can interfere electrochemical reaction. For example, proteins adsorbing on the electrode surface may raise impedance, [24] and the redox properties of proteins [25] will also influence electrochemical products. Thus, it is essential to explore whether the existence of proteins will disturb the ECL reactions on electrodes.…”

Section: Resultsmentioning

confidence: 99%

An In Situ Investigation of the Protein Corona Formation Kinetics of Single Nanomedicine Carriers by Self‐Regulated Electrochemiluminescence Microscopy

Xing

¹

,

Gou

²

,

Jiang

³

et al. 2023

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Protein corona extensively exists at the bio–nano interface due to the natural adsorption of proteins onto nanomaterials in biological fluids. Aside from the robust property of nanoparticles, the dynamics of the protein corona shell largely define their chemical identity by altering interface properties. However, the soft coronas are normally complex and rapidly changed. To real‐time trace the entire formation, we report here a self‐regulated electrochemiluminescence (ECL) microscopy based on the interaction of the Ru(bpy)33+ with nanoparticle surface. Thus, the heterogeneity in protein corona data is in situ observed on single nanoparticle “core” before and after loading drugs in nanomedicine carriers. The label‐free, optical stable and dynamic ECL microscopy minimize misinterpretations caused by the variation of nanoparticle size and polydispersity. Accordingly, the synergetic actions of proteins and nanoparticles properties are uncovered by chemically engineered protein corona. After comparing the protein corona formation kinetics in different complex systems and different nanomedicine carriers, the universality and accuracy of this technique were well demonstrated via the ECL kinetics curves regulated by competitive adsorption of Ru(bpy)33+ and multiple proteins on surface of various carriers. The work is of great significance for studying bio‐nano interface in drug delivery and targeted cancer treatment.

show abstract

An In Situ Investigation of the Protein Corona Formation Kinetics of Single Nanomedicine Carriers by Self‐Regulated Electrochemiluminescence Microscopy

Xing

¹

,

Gou

²

,

Jiang

³

et al. 2023

Angewandte Chemie

0

View full text Add to dashboard Cite

Protein corona extensively exists at the bio–nano interface due to the natural adsorption of proteins onto nanomaterials in biological fluids. Aside from the robust property of nanoparticles, the dynamics of the protein corona shell largely define their chemical identity by altering interface properties. However, the soft coronas are normally complex and rapidly changed. To real‐time trace the entire formation, we report here a self‐regulated electrochemiluminescence (ECL) microscopy based on the interaction of the Ru(bpy)33+ with nanoparticle surface. Thus, the heterogeneity in protein corona data is in situ observed on single nanoparticle “core” before and after loading drugs in nanomedicine carriers. The label‐free, optical stable and dynamic ECL microscopy minimize misinterpretations caused by the variation of nanoparticle size and polydispersity. Accordingly, the synergetic actions of proteins and nanoparticles properties are uncovered by chemically engineered protein corona. After comparing the protein corona formation kinetics in different complex systems and different nanomedicine carriers, the universality and accuracy of this technique were well demonstrated via the ECL kinetics curves regulated by competitive adsorption of Ru(bpy)33+ and multiple proteins on surface of various carriers. The work is of great significance for studying bio‐nano interface in drug delivery and targeted cancer treatment.

show abstract

Redox Denaturation of Proteins: Electrochemical Treatment of Egg Plasma

Cited by 4 publications

References 42 publications

Effects of electric charges on serum protein aggregation induced by a low temperature atmospheric pressure plasma

Effects of electric charges on serum protein aggregation induced by a low temperature atmospheric pressure plasma

An In Situ Investigation of the Protein Corona Formation Kinetics of Single Nanomedicine Carriers by Self‐Regulated Electrochemiluminescence Microscopy

An In Situ Investigation of the Protein Corona Formation Kinetics of Single Nanomedicine Carriers by Self‐Regulated Electrochemiluminescence Microscopy

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