Redox control of tumor cell apoptosis during hypoxia

Abstract

Currently, close attention is paid to studies aimed at searching for redox-sensitive targets for the regulation of tumor cell death. Tumor growth is characterized by impaired cell proliferation, differentiation, and apoptosis against the background of oxidative stress. Hypoxia contributes to the formation of mitochondrial dysfunction and acts as an additional factor that exacerbates oxidative stress in the tumor cell. Reactive oxygen species are general damaging factors, however, they can act as modulators of processes such as reception, intracellular signaling, proliferation, apoptosis, while taking part in the functioning of the cell redox system and contributing to the oxidative modification of macromolecules. One of the possible reasons for the activation of the production of reactive oxygen species is the low content of O2 in the cell, the final electron acceptor to ensure the functioning of the enzymes of the mitochondrial respiratory chain. The glutathione system makes a significant contribution to maintaining the balance between prooxidants and antioxidants in the cell. The role of this system is justified by the reduction potential of glutathione, which, acting as an acceptor of hydroxyl ions and singlet oxygen, significantly reduces the cytotoxic and damaging effects of reactive oxygen species. At the same time, it serves as a coenzyme for glutathione-dependent enzymes, which play a leading role not only in providing antioxidant processes, but also in maintaining the thiol disulfide balance. Hypoxia, which acts as a factor in the activation of free radical oxidation against the background of proliferation and apoptosis dysregulation, contributes to the formation of resistance of tumor cells to chemotherapeutic effects. In light of this, the importance of studying the redox-dependent mechanisms involved in the regulation and implementation of tumor cell death under insufficient oxygen supply becomes obvious, which is necessary for the development of personalized antitumor therapy. The article presents a review of modern literature, including the results of our own research, on the role of the thiol disulfide system and oxidatively modified proteins in the redox regulation of proliferation and apoptotic death of tumor cells, including under hypoxic conditions.