Redox Control of Apoptosis: An Update

Filomeni, Giuseppe; Ciriolo, Maria Rosa

doi:10.1089/ars.2006.8.2187

Cited by 81 publications

(57 citation statements)

References 58 publications

(56 reference statements)

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“…Oxidative assault can lead to inhibition of cell proliferation or even cell death (Filomeni and Ciriolo 2006;Domej et al 2006). To further analyze the extent of damage by uranium, LE cells were assessed for cell proliferation using MTT assay (Fig.…”

Section: Resultsmentioning

confidence: 99%

Uranium induces oxidative stress in lung epithelial cells

et al. 2006

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Uranium compounds are widely used in the nuclear fuel cycle, antitank weapons, tank armor, and also as a pigment to color ceramics and glass. Effective management of waste uranium compounds is necessary to prevent exposure to avoid adverse health effects on the population. Health risks associated with uranium exposure includes kidney disease and respiratory disorders. In addition, several published results have shown uranium or depleted uranium causes DNA damage, mutagenicity, cancer and neurological defects. In the current study, uranium toxicity was evaluated in rat lung epithelial cells. The study shows uranium induces significant oxidative stress in rat lung epithelial cells followed by concomitant decrease in the antioxidant potential of the cells. Treatment with uranium to rat lung epithelial cells also decreased cell proliferation after 72 h in culture. The decrease in cell proliferation was attributed to loss of total glutathione and superoxide dismutase in the presence of uranium. Thus the results indicate the ineffectiveness of antioxidant system's response to the oxidative stress induced by uranium in the cells.

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Section: Resultsmentioning

confidence: 99%

Uranium induces oxidative stress in lung epithelial cells

et al. 2006

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“…To validate the method also in those conditions where redox changes are caused indirectly, we explored the ability of our system to detect oxidation occurring during apoptosis. This is a well-described phenomenon caused by increased ROS production and, to some extent, by active extrusion of GSH (20,22). For this purpose, we used the tumor necrosis factor member TRAIL, which has been used for the same purpose in other studies (26).…”

Section: Fig 1 Validation Of the Redox Histochemistry Method (A)mentioning

confidence: 99%

Single-Cell Redox Imaging Demonstrates a Distinctive Response of Dopaminergic Neurons to Oxidative Insults

Horowitz

Milanese

Maio

et al. 2011

Antioxidants & Redox Signaling

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Aims: The study of the intracellular oxido-reductive (redox) state is of extreme relevance to the dopamine (DA) neurons of the substantia nigra pars compacta. These cells possess a distinct physiology intrinsically associated with elevated reactive oxygen species production, and they selectively degenerate in Parkinson's disease under oxidative stress conditions. To test the hypothesis that these cells display a unique redox response to mild, physiologically relevant oxidative insults when compared with other neuronal populations, we sought to develop a novel method for quantitatively assessing mild variations in intracellular redox state. Results: We have developed a new imaging strategy to study redox variations in single cells, which is sensitive enough to detect changes within the physiological range. We studied DA neurons' physiological redox response in biological systems of increasing complexity-from primary cultures to zebrafish larvae, to mammalian brains-and identified a redox response that is distinctive for substantia nigra pars compacta DA neurons. We studied simultaneously, and in the same cells, redox state and signaling activation and found that these phenomena are synchronized. Innovation: The redox histochemistry method we have developed allows for sensitive quantification of intracellular redox state in situ. As this method is compatible with traditional immunohistochemical techniques, it can be applied to diverse settings to investigate, in theory, any cell type of interest. Conclusion: Although the technique we have developed is of general interest, these findings provide insights into the biology of DA neurons in health and disease and may have implications for therapeutic intervention. Antioxid. Redox Signal. 15, 855-871.

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“…Indeed, in proliferating stage, Atox1 is present mostly in the reduced form (30). However, the GSH/GSSG-based redox potential may change, particularly during cell differentiation and polarization (69), serum depletion (86), or apoptosis (20) from -258 up to -165 mV (47). Such conditions would favor Atox1 oxidation and may significantly affect copper trafficking routes in cells.…”

Section: Redox Properties Of Atox1mentioning

confidence: 99%