2004
DOI: 10.1021/ja0438361
|View full text |Cite
|
Sign up to set email alerts
|

Redox Buffering of Hypochlorous Acid by Thiocyanate in Physiologic Fluids

Abstract: The major antimicrobial products of neutrophilic myeloperoxidase (MPO) in physiologic fluids are hypochlorous acid (HOCl) and hypothiocyanite (OSCN-), and the former is generally believed to be the killing agent. However, we have determined that HOCl oxidizes SCN- in a facile nonenzymic reaction. The observed kinetics and computational models substantiate the hypothesis that SCN- serves to moderate the potential autotoxicity of HOCl by restricting its lifetime in physiologic fluids. Furthermore, the oxidizing … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

1
99
2

Year Published

2006
2006
2020
2020

Publication Types

Select...
6
2
2

Relationship

0
10

Authors

Journals

citations
Cited by 109 publications
(105 citation statements)
references
References 16 publications
1
99
2
Order By: Relevance
“…In turn, SCN 2 in biological fluids serves as a scavenger of HOCl. HOCl can be generated by myeloperoxidase activity (15). SCN 2 rapidly reacts with HOCl to form HOSCN, which is a less reactive and more selective oxidant than HOCl, which may mitigate oxidative damage to SERCA by HOCl.…”
Section: Discussionmentioning
confidence: 99%
“…In turn, SCN 2 in biological fluids serves as a scavenger of HOCl. HOCl can be generated by myeloperoxidase activity (15). SCN 2 rapidly reacts with HOCl to form HOSCN, which is a less reactive and more selective oxidant than HOCl, which may mitigate oxidative damage to SERCA by HOCl.…”
Section: Discussionmentioning
confidence: 99%
“…Although MPO was previously thought to primarily use chloride as a substrate, it is now recognized that MPO also uses SCN À and that SCN À maybe the physiological substrate for MPO [27]. In addition, hypochlorite (OCl À ), the product of MPO oxidation of chloride, reacts almost instantaneously with SCN À at physiological concentrations [28] to give OSCN À suggesting that SCN À may have a central role not only in LPO antibacterial activity but also MPO antibacterial activity.…”
Section: Introductionmentioning
confidence: 99%
“…Additionally, SCN Ϫ rapidly scavenges OCl Ϫ without requiring catalysis (Fig. 1C) (31). In the absence of adequate SCN Ϫ , overproduction of OCl Ϫ by MPO during inflammation might result in severe lung injuries, and lead to the selfdestruction of white blood cells.…”
mentioning
confidence: 99%