Redox Behavior of Anticancer Chalcone on a Glassy Carbon Electrode and Evaluation of its Interaction Parameters with DNA

Abstract

Abstract:The interaction of anticancer chalcone [AMC, 1-(4'-aminophenyl)-3-(4-N,Ndimethylphenyl)-2-propen-1-one] with DNA has been explored using electrochemical, spectroscopic and viscometric techniques. A shift in peak potential and decrease in peak current were observed in cyclic voltammetry and hypochromism accompanied with bathochromic shift were noticed in UV-Vis absorption spectroscopy. These findings were taken as evidence for AMC -DNA intercalation. A binding constant (K) with a value of 6.15 × 10 5 M -1 was obtained from CV data, which was also confirmed by UV-Vis absorption titration. Moreover, the diffusion coefficient of the drug with and without DNA (D b and D u ), heterogeneous electron transfer rate constant (k o ) and electron affinity (A)were also calculated from electrochemical data.

Voltammetric and spectroscopic studies on the interaction of tilmicosin with bovine serum albumin at different pHs

“…The interactions between drug molecules and bioactive molecules, such as DNA, proteins and amino acids have aroused great interest in recent years [3][4][5][6][7][8]. Bovine serum albumin (BSA) has been one of the most extensively studied proteins [9].…”

Electrochemical behavior of 1-ferrocenyl-3-phenyl-2-propen-1-one on glassy carbon electrode and evaluation of its interaction parameters with DNA

“…These peculiar spectral characteristics are expected due to the overlap of the electronic states of the aromatic chromophore of the ferrocenylone with the electronic states of the DNA bases. 7 The aromatic n-p* and p-p* states of the planar group (-CO-C=C-C 6 H 5 ) attached to the cyclopentadienyl ring of ferrocene seem to interact strongly with the electronic states of the DNA bases causing red shift in the absorption, followed by an extensive hypochromic shift. The mode of interaction can be inferred from the shift in lmax.…”

“…Soc. 448 With these facts in mind and in continuation of our interest in chalcones due to their greater binding affinity with DNA, 7 we synthesized 1-ferrocenyl-3-phenyl-2-propen-1-one (Scheme 1) and evaluated its binding parameters with DNA with the objective of searching out an effective new anticancer drug.…”

Electrochemical investigations of unexplored anthraquinones and their DNA binding

“…Double stranded DNA was extracted from chicken blood by Falcon method [15,16]. The concentration of the DNA stock solution (2.0 mM) was determined from UV absorbance at 260 nm using a molar extinction coefficient (ε) of 6600 M −1 cm −1 [17,18]. A ratio of absorbance at 260 and 280 nm of (A 260 /A 280 ) > 1.8 indicated protein free DNA [19].…”