Redirection of tryptophan metabolism in tobacco by ectopic expression of an Arabidopsis indolic glucosinolate biosynthetic gene

Nonhebel, Heather M.; Yuan, Youxi; Al-Amier, H.; Pieck, Michael; Akor, Enne; Ahamed, Arifa; Cohen, Jerry D.; Celenza, John L.; Normanly, Jennifer

doi:10.1016/j.phytochem.2010.10.018

Cited by 28 publications

(24 citation statements)

References 67 publications

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“…S3B). From the analysis of DM-IPA and [ 13 C 11 , 15 N]DM-IPA, 95% of total IPA was efficiently labeled in this condition, in which 91% of total IAA was labeled (Fig. S3 C and D).…”

Section: Resultsmentioning

confidence: 96%

“…By using a synthetic [ 13 C 11 , 15 N]IPA as an internal standard, we quantified IPA levels in Arabidopsis. The level of IPA in 3-wk-old WT seedlings was 53.8 ± 7.5 ng/gfw (Figs.…”

Section: Resultsmentioning

confidence: 99%

“…We tested whether IAAld is mainly produced from Trp in Arabidopsis by feeding a [ 13 C 11 , 15 N 2 ]Trp to trp1-1 (Fig. S6A).…”

Section: Resultsmentioning

confidence: 99%

“…Recent studies indicated that the IAOx pathway operates in relatively few plant species that have CYP79B family members to convert Trp to IAOx (9-13). IAOx was identified in Arabidopsis, but not from CYP79B-deficient mutants and several noncrucifer plants (9,14,15). The IAM pathway has been suggested to exist widely in plants, but it remains unclear exactly how IAM is produced (16).…”

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confidence: 99%

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The main auxin biosynthesis pathway in Arabidopsis

Mashiguchi

Tanaka

Sakai

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

799

720

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The phytohormone auxin plays critical roles in the regulation of plant growth and development. Indole-3-acetic acid (IAA) has been recognized as the major auxin for more than 70 y. Although several pathways have been proposed, how auxin is synthesized in plants is still unclear. Previous genetic and enzymatic studies demonstrated that both TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) and YUCCA (YUC) flavin monooxygenase-like proteins are required for biosynthesis of IAA during plant development, but these enzymes were placed in two independent pathways. In this article, we demonstrate that the TAA family produces indole-3-pyruvic acid (IPA) and the YUC family functions in the conversion of IPA to IAA in Arabidopsis (Arabidopsis thaliana) by a quantification method of IPA using liquid chromatography-electrospray ionization-tandem MS. We further show that YUC protein expressed in Escherichia coli directly converts IPA to IAA. Indole-3-acetaldehyde is probably not a precursor of IAA in the IPA pathway. Our results indicate that YUC proteins catalyze a rate-limiting step of the IPA pathway, which is the main IAA biosynthesis pathway in Arabidopsis.plant hormone | metabolism

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“…S3B). From the analysis of DM-IPA and [ 13 C 11 , 15 N]DM-IPA, 95% of total IPA was efficiently labeled in this condition, in which 91% of total IAA was labeled (Fig. S3 C and D).…”

Section: Resultsmentioning

confidence: 96%

“…By using a synthetic [ 13 C 11 , 15 N]IPA as an internal standard, we quantified IPA levels in Arabidopsis. The level of IPA in 3-wk-old WT seedlings was 53.8 ± 7.5 ng/gfw (Figs.…”

Section: Resultsmentioning

confidence: 99%

“…We tested whether IAAld is mainly produced from Trp in Arabidopsis by feeding a [ 13 C 11 , 15 N 2 ]Trp to trp1-1 (Fig. S6A).…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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The main auxin biosynthesis pathway in Arabidopsis

Mashiguchi

Tanaka

Sakai

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

799

720

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show abstract

“…YUC) cannot compensate for the loss of TAA1 suggests that these proteins are active at different spatial and temporal sites during the plant life cycle (Tao et al, 2008). Interestingly, recent reports suggest that TAA/TAR and YUC genes function in the same auxin biosynthetic pathway (Strader and Bartel, 2008;Phillips et al, 2011) and also question the biochemical function of YUC in the tryptamine pathway (Tivendale et al, 2010;Nonhebel et al, 2011), highlighting how little we actually know regarding these pathways.…”

mentioning

confidence: 99%

Expression of ArabidopsisSHORT INTERNODES/STYLISHFamily Genes in Auxin Biosynthesis Zones of Aerial Organs Is Dependent on a GCC Box-Like Regulatory Element

et al. 2011

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Auxin/indole-3-acetic acid (IAA) biosynthesis in Arabidopsis (Arabidopsis thaliana) plays a major role in growth responses to developmental and genetic signals as well as to environmental stimuli. Knowledge of its regulation, however, remains rudimentary, and few proteins acting as transcriptional modulators of auxin biosynthesis have been identified. We have previously shown that alteration in the expression level of the SHORT INTERNODES/STYLISH (SHI/STY) family member STY1 affects IAA biosynthesis rates and IAA levels and that STY1 acts as a transcriptional activator of genes encoding auxin biosynthesis enzymes. Here, we have analyzed the upstream regulation of SHI/STY family members to gain further insight into transcriptional regulation of auxin biosynthesis. We attempted to modulate the normal expression pattern of STY1 by mutating a putative regulatory element, a GCC box, located in the proximal promoter region and conserved in most SHI/STY genes in Arabidopsis. Mutations in the GCC box abolish expression in aerial organs of the adult plant. We also show that induction of the transcriptional activator DORNRÖ SCHEN-LIKE (DRNL) activates the transcription of STY1 and other SHI/STY family members and that this activation is dependent on a functional GCC box. Additionally, STY1 expression in the strong drnl-2 mutant or the drn drnl-1 puchi-1 triple mutant, carrying knockdown mutations in both DRNL and its close paralogue DRN as well as one of their closest homologs, PUCHI, was significantly reduced, suggesting that DRNL regulates STY1 during normal plant development and that several other genes might have redundant functions.

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