Redirection of anthocyanin synthesis in Osteospermum hybrida by a two-enzyme manipulation strategy

Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin

“…These results reconfirm that pelargonidin production is an effective way to change flower color toward orange or bright red, as shown previously in petunia (Elomaa et al 1995;Meyer et al 1987;Tanaka et al 1995;Tsuda et al 2004), tobacco (Nakatsuka et al 2007), and Osteospermum (Seitz et al 2007). Our results confirm that downregulation of the F3Ј5ЈH and F3ЈH genes is insufficient and that addition of a suitable the proper DFR gene is necessary to generate a metabolic flux toward pelargonidin.…”

“…Downregulation of the F3Ј5ЈH gene and overexpression of the gerbera DFR gene in Osteospermum hybrida successfully redirects anthocyanin synthesis from delphinidin to pelargonidin (Seitz et al 2007). Pelargonium petals of all examined cultivars contain pelargonidin in addition to cyanidin and delphinidin (Kobayashi et al 1998).…”

Delivery of Multiple Transgenes to Plant Cells

“…Some of the approaches used for the production of transgenic plants carrying multiple new traits include (1) retransformation (e.g. Singla-Pareek et al, 2003;Seitz et al, 2007), the stacking of several transgenes by successive delivery of single genes into transgenic plants; (2) cotransformation (e.g. , the combined delivery of several transgenes in a single transformation experiment; and (3) sexual crosses (e.g.…”

RNA interference: concept to reality in crop improvement

“…Many researches are being performed to improve the flowers through gene silencing (Table 5). Seitz et al (2007) and Nakatsuka et al (2010) suggested that reduced accumulation of polyacylated anthocyanins by RNAi could cause modulations of flower colour. In 2005, Nishihara et al had applied RNAi-mediated silencing of chalcone isomerase (CHI) in tobacco and reported reduced pigmentation and change of flavonoid components in flower petals.…”