Redirecting Cell Fate During in vitro Embryogenesis: Phytoglobins as Molecular Switches

Elhiti, Mohamed; Mira, Mohamed M.; Hill, Robert D.; Stasolla, Claudio

doi:10.3389/fpls.2018.01477

Cited by 12 publications

(13 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is relevant to mention that high levels of NO can counteract SE induction, highly lightening the importance of balanced ROS/RNS homeodynamics in cells. Scavenging of NO by phytoglobins ( 66 , 67 ) is suggested to integrate oxidative stress and auxin metabolism with the acquisition of SE competence. In plants, NO is produced mainly by the cytosolic nitrate reductase (NR) and mitochondrial electron transport-mediated nitrite to NO reduction ( 68 ).…”

Section: Introductionmentioning

confidence: 99%

From Plant Survival Under Severe Stress to Anti-Viral Human Defense – A Perspective That Calls for Common Efforts

et al. 2021

View full text Add to dashboard Cite

Reprogramming of primary virus-infected cells is the critical step that turns viral attacks harmful to humans by initiating super-spreading at cell, organism and population levels. To develop early anti-viral therapies and proactive administration, it is important to understand the very first steps of this process. Plant somatic embryogenesis (SE) is the earliest and most studied model for de novo programming upon severe stress that, in contrast to virus attacks, promotes individual cell and organism survival. We argued that transcript level profiles of target genes established from in vitro SE induction as reference compared to virus-induced profiles can identify differential virus traits that link to harmful reprogramming. To validate this hypothesis, we selected a standard set of genes named ‘ReprogVirus’. This approach was recently applied and published. It resulted in identifying ‘CoV-MAC-TED’, a complex trait that is promising to support combating SARS-CoV-2-induced cell reprogramming in primary infected nose and mouth cells. In this perspective, we aim to explain the rationale of our scientific approach. We are highlighting relevant background knowledge on SE, emphasize the role of alternative oxidase in plant reprogramming and resilience as a learning tool for designing human virus-defense strategies and, present the list of selected genes. As an outlook, we announce wider data collection in a ‘ReprogVirus Platform’ to support anti-viral strategy design through common efforts.

show abstract

Section: Introductionmentioning

confidence: 99%

From Plant Survival Under Severe Stress to Anti-Viral Human Defense – A Perspective That Calls for Common Efforts

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Programmed cell death (PCD) is a conserved process occurring during plant embryogenesis, which is responsible for the dismantling of the suspensor and the removal of subordinate embryos in gymnosperm seeds [81,82]. During in vitro embryogenesis PCD eliminates specific embryogenic cells [83], and it is required for shaping the embryo body, as unequivocally demonstrated in spruce [84].…”

Section: Programmed Cell Death (Pcd) During Sementioning

confidence: 99%

Transduction of Signals during Somatic Embryogenesis

Elhiti

Stasolla

2022

Plants

Self Cite

View full text Add to dashboard Cite

Somatic embryogenesis (SE) is an in vitro biological process in which bipolar structures (somatic embryos) can be induced to form from somatic cells and regenerate into whole plants. Acquisition of the embryogenic potential in culture is initiated when some competent cells within the explants respond to inductive signals (mostly plant growth regulators, PRGs), and de-differentiate into embryogenic cells. Such cells, “canalized” into the embryogenic developmental pathway, are able to generate embryos comparable in structure and physiology to their in vivo counterparts. Genomic and transcriptomic studies have identified several pathways governing the initial stages of the embryogenic process. In this review, the authors emphasize the importance of the developmental signals required for the progression of embryo development, starting with the de-differentiation of somatic cells and culminating with tissue patterning during the formation of the embryo body. The action and interaction of PGRs are highlighted, along with the participation of master regulators, mostly transcription factors (TFs), and proteins involved in stress responses and the signal transduction required for the initiation of the embryogenic process.

show abstract

“…Chitosan is another plant growth regulators (PGR) that can be exogenously applied to enhance nitrogen metabolism and has a promotive role on chlorophylls and carotenoids, and is known to improve plant growth under salinity conditions (Bakhoum et al, 2020; Zayed et al, 2017). Phytoglobins (plant hemoglobins) are molecular switches which act as modulators of stress responses in plants by scavenging NO (Elhiti et al, 2018). The underlying mechanisms and possible roles of various PGRs have been discussed by Fariduddin et al (2019) and Bhat, Kumar, et al (2020).…”

Section: Salinity Stress Tolerance Mechanism In Plantsmentioning

confidence: 99%

Emerging warriors against salinity in plants: Nitric oxide and hydrogen sulphide

Goyal

Jhanghel

Mehrotra

2021

Physiologia Plantarum

View full text Add to dashboard Cite

The agriculture sector is vulnerable to various environmental stresses, which significantly affect plant growth, performance, and development. Abiotic stresses, such as salinity and drought, cause severe losses in crop productivity worldwide. Soil salinity is a major stress suppressing plant development through osmotic stress accompanied by ion toxicity, nutritional imbalance, and oxidative stress. Various defense mechanisms like osmolytes accumulations, activation of stress‐induced genes, and transcription factors, production of plant growth hormones, accumulation of antioxidants, and redox defense system in plants are responsible for combating salt stress. Nitric oxide (NO) and hydrogen sulphide (H2S) have emerged as novel bioactive gaseous signaling molecules that positively impact seed germination, homeostasis, plant metabolism, growth, and development, and are involved in several plant acclimation responses to impart stress tolerance in plants. NO and H2S trigger cell signaling by activating a cascade of biochemical events that result in plant tolerance to environmental stresses. NO‐ and H2S‐mediated signaling networks, interactions, and crosstalks facilitate stress tolerance in plants. Research on the roles and mechanisms of NO and H2S as challengers of salinity is entering an exponential exploration era. The present review focuses on the current knowledge of the mechanisms of stress tolerance in plants and the role of NO and H2S in adaptive plant responses to salt stress and provides an overview of the signaling mechanisms and interplay of NO and H2S in the regulation of growth and development as well as modulation of defense responses in plants and their long term priming effects for imparting salinity tolerance in plants.

show abstract

Redirecting Cell Fate During in vitro Embryogenesis: Phytoglobins as Molecular Switches

Cited by 12 publications

References 24 publications

From Plant Survival Under Severe Stress to Anti-Viral Human Defense – A Perspective That Calls for Common Efforts

From Plant Survival Under Severe Stress to Anti-Viral Human Defense – A Perspective That Calls for Common Efforts

Transduction of Signals during Somatic Embryogenesis

Emerging warriors against salinity in plants: Nitric oxide and hydrogen sulphide

Contact Info

Product

Resources

About