Redefinition of Nosema pyrausta (Perezia pyraustae Paillot 1927) basing upon ultrastructural and molecular phylogenetic studies

Tokarev, Yuri S.; Malysh, Julia M.; Kononchuk, A.G.; Seliverstova, Elena V.; Фролов, А. Н.; Issi, Irma V.

doi:10.1007/s00436-014-4272-3

Cited by 19 publications

(6 citation statements)

References 11 publications

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“…The use of primers flanking full-length gene of N. bombycis (Table 1) allowed amplification of this locus in N. bombycis, N. tyriae and N. pyrausta, that is expectable given that these three taxa are closely related and their SSU rRNA gene sequences are 99.7–99.8% similar (Tokarev et al ., 2015). The nucleotide sequence of N. bombycis was 100% identical to that of N. bombycis from B. mori (sampled in China) available at Genbank (# EOB11276).…”

Section: Resultsmentioning

confidence: 99%

“…HK showed clear differentiation between N.bombycis , N. pyrausta and N. tyriae that would be problematic if SSU rRNA was compared in these species due to the very low level of divergence of the latter locus (Tokarev et al ., 2015). Nosema antheraeae is more diverged in its SSU rRNA gene sequence from N. bombycis than N. tyriae and N. pyrausta and this is fairly reflected by differences in their HK gene sequences.…”

Section: Discussionmentioning

confidence: 99%

“…We, therefore, found it appropriate to test HK sequence information for bioinformatics studies and compare it with other molecular genetic loci such as SSU rRNA and RPB1, which are the most widely exploited genes in microsporidia studies. HK showed clear differentiation between N.bombycis, N. pyrausta and N. tyriae that would be problematic if SSU rRNA was compared in these species due to the very low level of divergence of the latter locus (Tokarev et al, 2015). Nosema antheraeae is more diverged in its SSU rRNA gene sequence from N. bombycis than N. tyriae and N. pyrausta and this is fairly reflected by differences in their HK gene sequences.…”

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confidence: 97%

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Hexokinase as a versatile molecular genetic marker for Microsporidia

et al. 2018

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Hexokinase (HK) is a core glycolytic enzyme of Microsporidia which regulates host cell metabolic processes. The goal of the present study was to test for the utility of HK for molecular phylogenetics, species identification and molecular detection of microsporidia in infected insects. HK sequence-based reconstructions were essentially similar to those based upon largest subunit RNA polymerase (RPB1) gene sequences, as well as previously published rRNA gene and genome-based trees. Comparing HK sequences allowed clear differentiation of closely related taxa, such as Nosema bombycis and Nosema pyrausta. In Nosema ceranae, unique SNPs were found for an isolate from wild colonies of the Burzyan dark honey bee as compared with the isolates from domesticated European honey bee. Similarly, in Encephalitozoon cuniculi, HK was as effective as RPB1 for discrimination of isolates belonging to different ITS genotypes. Amplification using species-specific primers flanking short fragments at the 3′-end of HK gene showed the presence of infection in insect tissues infected with N. pyrausta, Nosema ceranae and Paranosema (Antonospora) locustae. For the latter parasite species, HK expression was also demonstrated at early stages of infection using total mRNA extracts of locust larvae. These results indicate the suitability of HK as a novel tool for molecular genetic studies of Microsporidia.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 97%

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Hexokinase as a versatile molecular genetic marker for Microsporidia

et al. 2018

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“…The spores of the microsporidium used for experimental infection in this study were identified using SSU rRNA gene sequencing as N. pyrausta (Tokarev et al, 2015). When infected with an average dosage of 10 5 fresh spores per larva, the second instar treatment group (Lii) of O. nubilalis showed a median lethal time (LT 50 ) of 15.2 AE 1.27 days (mean AE SE) and 100% larval mortality, corresponding to 0% pupation success.…”

Section: Resultsmentioning

confidence: 99%

Transovarial transmission of Nosema pyrausta in three generations of Ostrinia nubilalis in laboratory tests

Grushevaya

Senderskiy

Zubarev

et al. 2021

Entomologia Exp Applicata

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The microsporidium Nosema pyrausta (Paillot) (Nosematidae) is a widespread and persistent entomopathogen with integrated pest management implications. Nosema pyrausta is capable of horizontal and vertical transmissions in Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) populations. After experimental per os infection, N. pyrausta virulence decreased with the instar of the host larvae used. At a dosage of 100,000 spores per fourth instar, 47% pupae and 36% adults survived and gave rise to transovarially infected offspring. The infection persisted through three laboratory generations of the host with prevalence levels ranging between 29 and 92%. Mature microsporidian spores were not found in smears prepared from the eggs laid by infected females, but cryosections demonstrated parasite cells within the host egg’s cytoplasm. A short region of N. pyrausta hexokinase was amplified using cDNA samples from infected females’ eggs, confirming the expression of the parasite’s DNA. Thus, vertical transmission of N. pyrausta is achieved through infection of insect eggs with prespore stages of the parasite.

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“…Nosema pyrausta [ 21 ] was propagated in a temporary laboratory culture of the European corn borer, Ostrinia nubilalis Hbn, which is the typical host of this parasite. The larvae were maintained on an artificial diet [ 22 ] and infected with N. pyrausta spores according to Tokarev et al [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Susceptibility of the Gypsy Moth Lymantria dispar (Lepidoptera: Erebidae) to Nosema pyrausta (Microsporidia: Nosematidae)

Kononchuk

Martemyanov

Ignatieva

et al. 2021

Insects

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The gypsy moth, Lymantria dispar, is a notorious forest defoliator, and various pathogens are known to act as natural regulators of its population density. As a widespread herbivore with a broad range of inhabited areas and host plants, it is potentially exposed to parasitic microorganisms from other insect hosts. In the present paper, we determined the susceptibility of gypsy moth larvae to the microsporidium Nosema pyrausta from the European corn borer, Ostrinia nubilalis. Gypsy moth samples from two localities of Western Siberia were used. N. pyrausta developed infections in the salivary gland and adipose tissue of gypsy moth prepupae and pupae, forming spore masses after 30 days of alimentary exposure to the second instar larvae. Among the experimental groups, the infection levels ranged from 0 to 9.5%. Effects of a covert baculovirus infection, phenylthiourea pretreatment and feeding insects on an artificial diet versus natural foliage were not significant in terms of microsporidia prevalence levels. Thus, L. dispar showed a low level of susceptibility to a non-specific microsporidium. It can be referred to as a resistant model host and not an appropriate substitute host for laboratory propagation of the microsporidium.

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Redefinition of Nosema pyrausta (Perezia pyraustae Paillot 1927) basing upon ultrastructural and molecular phylogenetic studies

Cited by 19 publications

References 11 publications

Hexokinase as a versatile molecular genetic marker for Microsporidia

Hexokinase as a versatile molecular genetic marker for Microsporidia

Transovarial transmission of Nosema pyrausta in three generations of Ostrinia nubilalis in laboratory tests

Susceptibility of the Gypsy Moth Lymantria dispar (Lepidoptera: Erebidae) to Nosema pyrausta (Microsporidia: Nosematidae)

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