Red Pepper (<i>Capsicum annuum</i>L.) Seed Extract Decreased Hepatic Gluconeogenesis and Increased Muscle Glucose Uptake<i>In Vitro</i>

Kim, Haeun; Cho, Kae Won; Jeong, Jae–Tack; Park, Kibeum; Ryu, Yungsun; Moyo, Knowledge Mudhibadhi; Kim, Hyun Kyung; Go, Gyungchoon

doi:10.1089/jmf.2017.4065

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…The protein levels were eliminated by RPSE supplementation; PEPCK and G6Pase were reduced by 43% (p < 0.001) and 78% (p < 0.01), respectively, in ObD mice. Our previous in vitro study also demonstrated that RPSE reduced gluconeogenesis by inhibiting PEPCK and G6Pase proteins in AML12 murine hepatocytes [19]. These novel findings proved that RPSE supplementation transcriptionally downregulated the expression of gluconeogenic genes and proteins, including PEPCK and GP6Pase, in the liver of ObD mice.…”

Section: Rpse Downregulated Hepatic Gluconeogenic Genes and Proteins supporting

confidence: 52%

“…As such, improved glucose uptake by RPSE supplementation in the ObD model was an innovative discovery. We previously demonstrated that 200 µg/mL of RPSE activated the translocation of GLUT4 to the plasma membrane followed by increasing glucose uptake in the C2C12 murine myotubes [ 19 ]. Similarly, there was a previous report that red pepper powder extracted using 70% ethanol improved insulin sensitivity and glycogen storage in liver and muscle tissues of ovariectomized rats fed a HFD [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

“…RPSE was provided by Novarex, Inc. (Seoul, Korea), and it was prepared as previously described [19]. In brief, the company purchased the red pepper seed cultivated in Yeong-yang from Yeong Yang Red Pepper Trade Corp (Korea).…”

Section: Animals and Dietsmentioning

confidence: 99%

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Red Pepper (Capsicum annuum L.) Seed Extract Improves Glycemic Control by Inhibiting Hepatic Gluconeogenesis via Phosphorylation of FOXO1 and AMPK in Obese Diabetic db/db Mice

et al. 2020

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Obesity is a notable risk factor for developing type 2 diabetes, augmenting the concern of obese diabetes (ObD). Anti-obesity and antioxidant effects of red pepper seeds extract (RPSE) have increased our expectations that RPSE would also improve the pathological phenotypes of obese diabetes. Therefore, we hypothesized that RPSE would have an anti-diabetic effect in ObD mice. Animals were assigned either as follows: (1) db/+, (2) db/db control, (3) RPSE (200 mg/kg bw), or (4) a comparative control (metformin 150 mg/kg bw). RPSE was orally administered daily for 8 weeks. As a result, RPSE supplementation improved diabetic phenotypes, including fasting glucose, hemoglobin (HbA1c), and insulin levels. Pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6), and triglycerides were reduced in RPSE-treated mice. RPSE supplementation also diminished the rate-limiting enzymes of gluconeogenesis, including glucose 6-phosphatas (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), in the liver. RPSE supplementation increased the phosphorylation of forkhead box protein O1 (FOXO1) and AMP-activated protein kinase (AMPK), which underlined the mechanism of the anti-diabetic effects of RPSE. Taken together, RPSE has the potential to improve glycemic control by repressing hepatic gluconeogenesis via the phosphorylation of FOXO1 and AMPK in ObD mice.

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Section: Rpse Downregulated Hepatic Gluconeogenic Genes and Proteins supporting

confidence: 52%

Section: Resultsmentioning

confidence: 99%

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Red Pepper (Capsicum annuum L.) Seed Extract Improves Glycemic Control by Inhibiting Hepatic Gluconeogenesis via Phosphorylation of FOXO1 and AMPK in Obese Diabetic db/db Mice

et al. 2020

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“…The regulatory effects of natural compounds on gluconeogenic pathways in hepatocytes have been reported previously. The red pepper seed extract treatment decreased the mRNA levels of G6PC and PCK1 and suppressed glucose production in AML-12 cells [ 17 ]. However, whether the inhibition of HGP was caused by the regulation of the gluconeogenic pathway was unclear.…”

Section: Discussionmentioning

confidence: 99%

“…The HGP assay was conducted according to a previous study, with modifications [ 17 ]. AML-12 cells were seeded in a 6-well plate.…”

Section: Methodsmentioning

confidence: 99%

Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells

Liao¹,

Cao²,

Xia³

et al. 2022

IJERPH

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Pea protein is considered to be a high quality dietary protein source, but also it is an ideal raw material for the production of bioactive peptides. Although the hypoglycemic effect of pea protein hydrolysate (PPH) has been previously reported, the underlying mechanisms, in particular its effect on the hepatic gluconeogenesis, remain to be elucidated. In the present study, we found that PPH suppressed glucose production in mouse liver cell-line AML-12 cells. Although both of the gluconeogenic and insulin signaling pathways in the AML-12 cells could be regulated by PPH, the suppression of glucose production was dependent on the inhibition of the cAMP response element-binding protein (CREB)-mediated signaling in the gluconeogenic pathway, but not the activation of insulin signaling. Findings from the present study have unveiled a novel role of PPH underlying its anti-diabetic activity, which could be helpful to accelerate the development of functional foods and nutraceuticals using PPH as a starting material.

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Molecular networks underlying cannabinoid signaling in skeletal muscle plasticity

Dalle

Schouten

Meeus

et al. 2022

Journal Cellular Physiology

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The cannabinoid system is ubiquitously present and is classically considered to engage in neural and immunity processes. Yet, the role of the cannabinoid system in the whole body and tissue metabolism via central and peripheral mechanisms is increasingly recognized. The present review provides insights in (i) how cannabinoid signaling is regulated via receptor-independent and -dependent mechanisms and (ii) how these signaling cascades (might) affect skeletal muscle plasticity and physiology.Receptor-independent mechanisms include endocannabinoid metabolism to eicosanoids and the regulation of ion channels. Alternatively, endocannabinoids can act as ligands for different classic (cannabinoid receptor 1 [CB 1 ], CB 2 ) and/or alternative (e.g., TRPV1, GPR55) cannabinoid receptors with a unique affinity, specificity, and intracellular signaling cascade (often tissue-specific). Antagonism of CB 1 might hold clues to improve oxidative (mitochondrial) metabolism, insulin sensitivity, satellite cell growth, and muscle anabolism, whereas CB 2 agonism might be a promising way to stimulate muscle metabolism and muscle cell growth. Besides, CB 2 ameliorates muscle regeneration via macrophage polarization toward an anti-inflammatory phenotype, induction of MyoD and myogenin expression and antifibrotic mechanisms. Also TRPV1 and GPR55 contribute to the regulation of muscle growth and metabolism. Future studies should reveal how the cannabinoid system can be targeted to improve muscle quantity and/or quality in conditions such as ageing, disease, disuse, and metabolic dysregulation, taking into account challenges that are inherent to modulation of the cannabinoid system, such as central and peripheral side effects.

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Red Pepper (Capsicum annuumL.) Seed Extract Decreased Hepatic Gluconeogenesis and Increased Muscle Glucose UptakeIn Vitro

Cited by 7 publications

References 26 publications

Red Pepper (Capsicum annuum L.) Seed Extract Improves Glycemic Control by Inhibiting Hepatic Gluconeogenesis via Phosphorylation of FOXO1 and AMPK in Obese Diabetic db/db Mice

Red Pepper (Capsicum annuum L.) Seed Extract Improves Glycemic Control by Inhibiting Hepatic Gluconeogenesis via Phosphorylation of FOXO1 and AMPK in Obese Diabetic db/db Mice

Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells

Molecular networks underlying cannabinoid signaling in skeletal muscle plasticity

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