Red Light Modulates Ultraviolet-Induced Gene Expression in the Epidermis of Hairless Mice

Abstract

Objective: The purpose of this study was to investigate whether low-level light therapy (LLLT) was capable of modulating expression of ultraviolet (UV) light-responsive genes in vivo. Materials and methods: The effects of 670 nm light-emitting diode (LED) array irradiation were investigated in a hairless SHK-1 mouse epidermis model. Mice were given a single dose of UVA/UVB light, or three doses of red light (670 nm @ 8 mW/cm 2 x 312 sec, 2.5 J/cm 2 per session) spread over 24 h along with combinations of pre-and post-UV treatment with red light. Levels of 14 UV-responsive mRNAs were quantified 24 h after UV irradiation by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results: The transcription of mRNAs encoding for cluster of differentiation molecule 11b (CD11b) ( p < 0.05) and interferon (IFN)-c ( p < 0.012) increased after irradiation with red light alone, whereas expression level of cyclooxygenase (COX)-2 ( p < 0.02) was downregulated. Genes unresponsive to UV did not change their expression levels after exposure to red light either. Pretreatment with red light significantly modified response of Fos to UV exposure ( p < 0.01). A synergy of UV and post-treatment with red light in reducing the transcription levels of CD11b ( p < 0.05) and inducible nitric oxide synthase (iNOS) ( p < 0.05) was observed. Conclusions: This is an initial observation that in mouse red light LLLT more often than not causes opposite gene expression changes or reduces those caused by moderate UVA-UVB irradiation.

Skin photorejuvenation effects of light-emitting diodes (LEDs): a comparative study of yellow and red LEDs in vitro and in vivo

“…reported that photodynamic therapy, using wavelengths of 613–645 nm, significantly induced the expression of the pro‐inflammatory cytokines, IL‐1β, TNF‐α and TGF‐β . Furthermore, in a recent study, expression of Fos, the key molecular component contributing to DNA damage after exposure to UV light, decreased after treatment with red light (670 nm) …”

Luminescent Nanomaterials for Energy-Efficient Display and Healthcare

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On The Existence of The DNA Resonance Code and Its Possible Mechanistic Connection to The Neural Code

“…We believe that the methods of modern genomics will be sufficient for decoding the resonance code of DNA: genetic engineering with subsequent measurement of spectral properties and full-genome mapping of the influence of waves on the openness and transcription of DNA. Toward that goal, we have shown the effect of red light on the expression of candidate genes in the murine epidermis (Myakishev-Rempel et al, 2015).…”