Red LED light treatment promotes cognitive learning through up-regulation of trpm4 in zebrafish

You, May Su; Yang, Wen Bin; Cheng, Chao; Yu, Sebastian; Chang, Han Chao; Yu, Hsin Su

doi:10.1016/j.jphotobiol.2020.112073

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…In mammals, TRPM4 gating appears to be modulated by heat, but data are limited to a single study ( Talavera et al 2005 ). In teleosts, TRPM4 is associated with learning in response to light stimuli and inflammation, but is otherwise uncharacterized ( You et al 2020 ; Li et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Evolution of Transient Receptor Potential (TRP) Ion Channels in Antarctic Fishes (Cryonotothenioidea) and Identification of Putative Thermosensors

York

Zakon

2022

Genome Biology and Evolution

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Animals rely on their sensory systems to inform them of ecologically relevant environmental variation. In the Southern Ocean, the thermal environment has remained between -1.9 and 5 °C for 15 million years, yet we have no knowledge of how an Antarctic marine organism might sense their thermal habitat as we have yet to discover a thermosensitive ion channel that gates (opens/closes) below 10 °C. Here, we investigate the evolutionary dynamics of transient receptor potential (TRP) channels, which are the primary thermosensors in animals, within cryonotothenioid fishes—the dominant fish fauna of the Southern Ocean. We found cryonotothenioids have a similar complement of TRP channels as other teleosts (∼28 genes). Previous work has shown that thermosensitive-gating in a given channel is species specific, and multiple channels act together to sense the thermal environment. Therefore, we combined evidence of changes in selective pressure, gene gain/loss dynamics, and the first sensory ganglion transcriptome in this clade to identify the best candidate TRP channels that might have a functional dynamic range relevant for frigid Antarctic temperatures. We concluded that TRPV1a, TRPA1b, and TRPM4 are the likeliest putative thermosensors, and found evidence of diversifying selection at sites across these proteins. We also put forward hypotheses for molecular mechanisms of other cryonotothenioid adaptations, such as reduced skeletal calcium deposition, sensing oxidative stress, and unusual magnesium homeostasis. By completing a comprehensive and unbiased survey of these genes, we lay the groundwork for functional characterization and answering long-standing thermodynamic questions of thermosensitive gating and protein adaptation to low temperatures.

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Section: Discussionmentioning

confidence: 99%

Evolution of Transient Receptor Potential (TRP) Ion Channels in Antarctic Fishes (Cryonotothenioidea) and Identification of Putative Thermosensors

York

Zakon

2022

Genome Biology and Evolution

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“…Red lighting was chosen based on the sensitivity of the zebrafish, where zebrafish favor red over other colors. However between the green and blue colors, green was the most preferred, with the fish having a strong aversion to the blue color 13 , 17 , 19 , 40 . For simple conditioning, only one divider was used to separate the food from the remaining space in the tank.…”

Section: Methodsmentioning

confidence: 99%

Zebrafish automatic monitoring system for conditioning and behavioral analysis

Barreiros

Barbosa

Dantas

et al. 2021

Sci Rep

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Studies using zebrafish (Danio rerio) in neuro-behavioural research are growing. Measuring fish behavior by computational methods is one of the most efficient ways to avoid human bias in experimental analyses, extending them to various approaches. Sometimes, thorough analyses are difficult to do, as fish can behave unpredictably during an experimental strategy. However, the analyses can be implemented in an automated way, using an online strategy and video processing for a complete assessment of the zebrafish behavior, based on the detection and tracking of fish during an activity. Here, a fully automatic conditioning and detailed analysis of zebrafish behavior is presented. Microcontrolled components were used to control the delivery of visual and sound stimuli, in addition to the concise amounts of food after conditioned stimuli for adult zebrafish groups in a conventional tank. The images were captured and processed for automatic detection of the fish, and the training of the fish was done in two evaluation strategies: simple and complex. In simple conditioning, the zebrafish showed significant responses from the second attempt, learning that the conditioned stimulus was a predictor of food presentation in a specific space of the tank, where the food was dumped. When the fish were subjected to two stimuli for decision-making in the food reward, the zebrafish obtained better responses to red light stimuli in relation to vibration. The behavior change was clear in stimulated fish in relation to the control group, thus, the distances traveled and the speed were greater, while the polarization was lower in stimulated fish. This automated system allows for the conditioning and assessment of zebrafish behavior online, with greater stability in experiments, and in the analysis of the behavior of individual fish or fish schools, including learning and memory studies.

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“…Studies on TRP channel function in fishes have focused on zebrafish ( Danio rerio ; e.g. [ 22 , 23 ]) and found that both TRPV1a and TRPA1b are expressed in sensory neurons in zebrafish larvae [ 24 , 25 ]. These studies found knockdowns of TRPV1a , but not TRPA1b , modulate thermal preference behaviour of larval zebrafish.…”

Section: Introductionmentioning

confidence: 99%

Temperature activated transient receptor potential ion channels from Antarctic fishes

York

2023

Open Biol.

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Antarctic notothenioid fishes (cryonotothenioids) live in waters that range between −1.86°C and an extreme maximum +4°C. Evidence suggests these fish sense temperature peripherally, but the molecular mechanism of temperature sensation in unknown. Previous work identified transient receptor potential (TRP) channels TRPA1b, TRPM4 and TRPV1a as the top candidates for temperature sensors. Here, cryonotothenioid TRPA1b and TRPV1a are characterized using Xenopus oocyte electrophysiology. TRPA1b and TRPV1a showed heat-evoked currents with Q10s of 11.1 ± 2.2 and 20.5 ± 2.4, respectively. Unexpectedly, heat activation occurred at a threshold of 22.9 ± 1.3°C for TRPA1b and 32.1 ± 0.6°C for TRPV1a. These fish have not experienced such temperatures for at least 15 Myr. Either (1) another molecular mechanism underlies temperature sensation, (2) these fishes do not sense temperatures below these thresholds despite having lethal limits as low as 5°C, or (3) native cellular conditions modify the TRP channels to function at relevant temperatures. The effects of osmolytes, pH, oxidation, phosphorylation, lipids and accessory proteins were tested. No conditions shifted the activity range of TRPV1a. Oxidation in combination with reduced cholesterol significantly dropped activation threshold of TRPA1b to 11.3 ± 2.3°C, it is hypothesized the effect may be due to lipid raft disruption.

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Red LED light treatment promotes cognitive learning through up-regulation of trpm4 in zebrafish

Cited by 6 publications

References 46 publications

Evolution of Transient Receptor Potential (TRP) Ion Channels in Antarctic Fishes (Cryonotothenioidea) and Identification of Putative Thermosensors

Evolution of Transient Receptor Potential (TRP) Ion Channels in Antarctic Fishes (Cryonotothenioidea) and Identification of Putative Thermosensors

Zebrafish automatic monitoring system for conditioning and behavioral analysis

Temperature activated transient receptor potential ion channels from Antarctic fishes

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