Red Grape Polyphenol Oral Administration Improves Immune Response in Women Affected by Nickel-Mediated Allergic Contact Dermatitis

Abstract

Background: Our previous findings demonstrated that in vitro supplementation of polyphenols, extracted from seeds of red grape (Nero di Troia cultivar), to peripheral lymphomonocytes from patients affected by allergic contact dermatitis (ACD) to nickel (Ni) could reduce release of pro-inflammatory cytokines and nitric oxide (NO), while increasing levels of interleukin (IL)-10, an anti-inflammatory cytokine. Objective: To assess whether an intervention with oral administration of polyphenols leads to a reduction of peripheral biomarkers in ACD patients. Method: At T0, 25 patients affected by ACD to Ni were orally administered with 300 mg polyphenols prodie extracted from seeds of red grape (Nero di Troia cultivar) (NATUR-OX®) for 3 months (T1). Other 25 patients affected by ACD to Ni received placebo only for the same period of time. Serum biomarkers were analyzed at T0 and T1. In both groups seven drop outs were recorded. Result: At T1 in comparison to T0, in treated patients, values of IFN-γ, IL-4, IL-17, PTX3 and NO decreased, while IL-10 levels increased when compared with T0 values. Conversely, in placebo-treated patients no modifications of biomarkers were evaluated at T1. Conclusion: Present laboratory data rely on the anti-oxidant, anti-inflammatory and anti-allergic properties of polyphenols.

Effects of Herbal Medicines on the Prevention and Treatment of Contact Dermatitis: A Systematic Review

“…Nine clinical trials examined the effects of various forms of herbal products, such as fresh herbs, creams, capsules, solutions, emulsions, injections, and soaps on CD. Among them, 5 studies evaluated the therapeutic measures ( 10 , 15 , 18 , 21 , 22 ) and 4 studies evaluated the preventive measures on CD ( 16 , 17 , 19 , 20 ).…”

“…In 5 studies, eligible participants were allocated into two groups (intervention and control) ( 10 , 15 , 18 , 21 , 22 ) whereas in 4 studies, the intervention and control were used in two different areas of the same body ( 16 , 17 , 19 , 20 ).…”

“…The articles were published from 2010 to 2020 (10,15–22). Three of the studies were performed in Iran ( 10 , 18 , 22 ), two of them were conducted in America ( 16 , 17 ), and the others were performed in Sweden, China, Thailand, and Italy ( 15 , 19 – 21 ).…”

Recent Advances on the Anti-inflammatory and Anti-oxidant properties of Red Grape Polyphenols: In Vitro and in Vivo Studies

“…Polyphenols extracted from seeds of red grape (Nero di Troia cultivar), when in vitro incubated with peripheral blood lymphomonocytes from patients with Ni-mediated CAD, reduced release of NO, IL-17 and IFN-, while enhancing IL-10 production, thus, exerting anti-oxidant and anti-inflammatory activities [51]. In a clinical trial, oral administration of Nero di Troia red grape polyphenols to patients with Ni-mediated CAD confirmed in vitro experiments in that they decreased serum levels of IFN-, IL-4, IL-17, NO and pentraxin 3, while levels of IL-10 were augmented [212]. This nutraceutical regimen led to an amelioration of CAD cutaneous manifestations.…”

Recent Advances on the Anti-Inflammatory and Antioxidant Properties of Red Grape Polyphenols: In Vitro and In Vivo Studies

“…Polyphenols extracted from seeds of red grape (Nero di Troia cultivar), when in vitro incubated with peripheral blood lymphomonocytes from patients with Ni-mediated CAD, reduced release of NO, IL-17 and IFN-γ, whereas enhancing IL-10 production they exerted antioxidant and anti-inflammatory activities [51]. In a clinical trial, oral administration of Nero di Troia red grape polyphenols to patients with Ni-mediated CAD confirmed in vitro experiments in that they decreased serum levels of IFN-γ, IL-4, IL-17, NO, and pentraxin 3, whereas levels of IL-10 were augmented [217]. This nutraceutical regimen led to an amelioration of CAD cutaneous manifestations.…”