“Red complex” (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola ) in endodontic infections: A molecular approach

Rôças, Isabela N.; Siqueira, José F.; Santos, Kátia Regina Netto dos; Coelho, Ana; Janeiro, Río de

doi:10.1067/moe.2001.114379

Cited by 129 publications

(76 citation statements)

References 13 publications

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“…Additionally, these studies have elucidated previously unappreciated species. Next-generation sequencing (NGS) technologies have revealed a greater complexity within the periodontal disease microbiome, well beyond the traditional "red complex" that is comprised of Tannerella forsythia, Porphyromonas gingivalis, and Treponema denticola [25]. Microbiome studies have shifted this dogma, showing the prevalence of many diseaseassociated genera and species, including but not limited to Spirochetes, Filifactor, and Fusobacterium, highlighting a more diverse disease community than previously considered [19,26].…”

Section: Identifying the Usual Suspects And Beyondmentioning

confidence: 99%

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Delaney

Kean

Short

et al. 2018

Curr Clin Micro Rpt

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Purpose of Review Over the last decade, microbiome studies have enhanced our knowledge and understanding of the polymicrobial nature of oral infections. Recently, profiling of the fungal microbiome has expanded our conventional understanding of oral ecology, revealing the critical importance of yeasts within this complex microbiome. This review aims to explore our current appreciation of interkingdom interactions in oral disease. Recent Findings There is a growing evidence base of interactions and pathogenic synergy and antagonism with bacterial species within oral disease. Recent studies have helped to develop our knowledge of how Candida albicans, alongside bacteria such as Porphyromonas gingivalis, Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, and Lactobacillus species, influence overall pathogenicity. Summary Clinical and experimental evidence makes a compelling case for a role for C. albicans in a number of oral infections, though whether its role is an active accomplice or passive bystander remains to be determined.

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Section: Identifying the Usual Suspects And Beyondmentioning

confidence: 99%

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Delaney

Kean

Short

et al. 2018

Curr Clin Micro Rpt

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“…Cells were washed twice by centrifugation in TE buffer at 2500 g for 10 min. The cells were resuspended and lysed with either SDS 10% and proteinase K (Sigma) 20 mg=ml for gram-negative strains or in 150 ìl of an enzyme mixture containing lysozyme (Sigma) 15 resuspended by sonication for 15 s and incubated at 378C for 1 h. DNA was isolated and purified by the method described by Smith et al [26]. The concentration of the purified DNA was determined by spectrophotometric measurement of the absorbance at 260 nm.…”

Section: Dna Extraction and Preparation Of Dna Probesmentioning

confidence: 99%

“…In recent years, molecular methods have also been used to investigate the microbiota associated with infected root canals and with acute periapical abscesses [2,[9][10][11][12][13][14][15][16][17][18][19][20]. These methods have provided significant additional knowledge regarding the composition of the endodontic microbiota.…”

Section: Introductionmentioning

confidence: 99%

“…These methods have provided significant additional knowledge regarding the composition of the endodontic microbiota. For instance, molecular technologies have allowed for the first time the detection of B. forsythus [2,9,15], T. denticola [15,16], T. maltophilum [12], and Pr. tannerae [20] in infected root canals in relatively high prevalences.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of 16S rDNA-based PCR and checkerboard DNA–DNA hybridisation for detection of selected endodontic pathogens

Siqueira

Rôças

Uzeda

et al. 2002

Journal of Medical Microbiology

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Molecular methods have been used recently to investigate the bacteria encountered in human endodontic infections. The aim of the present study was to compare the ability of a 16S rDNA-based PCR assay and checkerboard DNA-DNA hybridisation in detecting Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Peptostreptococcus micros, Porphyromonas endodontalis, Por. gingivalis and Treponema denticola directly from clinical samples. Specimens were obtained from 50 cases of endodontic infections and the presence of the target species was investigated by whole genomic DNA probes and checkerboard DNA-DNA hybridisation or taxon-specific oligonucleotides with PCR assay. Prevalence of the target species was based on data obtained by each method. The sensitivity and specificity of each molecular method was compared with the data generated by the other method as the reference -a value of 1.0 representing total agreement with the chosen standard. The methods were also compared with regard to the prevalence values for each target species. Regardless of the detection method used, T. denticola, Por. gingivalis, Por. endodontalis and B. forsythus were the most prevalent species. If the checkerboard data for these four species were used as the reference, PCR detection sensitivities ranged from 0.53 to 1.0, and specificities from 0.5 to 0.88, depending on the target bacterial species. When PCR data for the same species were used as the reference, the detection sensitivities for the checkerboard method ranged from 0.17 to 0.73, and specificities from 0.75 to 1.0. Accuracy values ranged from 0.6 to 0.74. On the whole, matching results between the two molecular methods ranged from 60% to 97.5%, depending on the target species. The major discrepancies between the methods comprised a number of PCR-positive but checkerboard-negative results. Significantly higher prevalence figures for Por. endodontalis and T. denticola were observed after PCR assessment. There was no further significant difference between the methods with regard to detection of the other target species.

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“…Its association with treatment failure in root-filled teeth is reported to be significant (2). In addition to E. faecalis, several other bacterial pathogens including Peptostreptococcus micros, Porphyromonas gingivalis/ endodontalis, Fusobacterium necrophorum/nucleatum, Prevotella intermedia/nigrescens, Tannerella forsythia (Bacteroides forsythus), Streptococcus species, and also Candida albicans have been reported to be involved in mixed infections of necrotic root canals and other periradicular lesions (3,(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of Enterococcus faecalis from necrotic root canals using multiplex PCR

et al. 2007

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This study was designed to survey the incidence of Enterococcus faecalis infection in symptomatic and asymptomatic root canals of necrotic teeth using PCR and to isolate the bacterium for further screening. Sixty patients categorized according to their clinical symptoms were used for sampling by insertion of paper points into the root canals and absorbing all the fluids present within them. The samples were incubated in 1.0 ml 2xYT (containing 16 g bacto tryptone, 10 g yeast extract and 5.0 g NaCl per liter) for 24 h at 37°C without aeration prior to multiplex PCR analysis. To assist the isolation of E. faecalis, subsamples were further grown in the same medium supplemented with 6.5% NaCl and back-inoculated into bile esculin. Using multiple cultivation-dependent and PCR analyses, 6 cases (10%) of E. faecalis were identified. Four isolates were obtained from asymptomatic cases of chronic apical periodontitis, and the other two were associated with phoenix abscess and acute apical abscess, respectively. No E. faecalis infection was found in 5 patients with acute apical periodontitis or in 9 with chronic suppurative periodontitis. Our results indicate that there is no significant difference in the incidence of E. faecalis between symptomatic and asymptomatic necrotic dental root canals (P > 0.05). (J. Oral Sci. 49, [221][222][223][224][225][226][227] 2007)

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“Red complex” (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola ) in endodontic infections: A molecular approach

Cited by 129 publications

References 13 publications

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Comparison of 16S rDNA-based PCR and checkerboard DNA–DNA hybridisation for detection of selected endodontic pathogens

Isolation and identification of Enterococcus faecalis from necrotic root canals using multiplex PCR

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