Red Cell Preservation in Protein-Poor Media

Abstract

Red cells stored under blood bank conditions normally show less than 1% spontaneous in vitro hemolysis even after 5 weeks; larger hemolysis may be found if the cells are suspended and stored in a saline-adenine-glucose (SAG) solution with very little trapped plasma. Delay of the addition of the suspension medium, return of 25 ml plasma after a maximal plasma harvest, addition of mannitol 10-30 mmol·1^-1 to the suspension medium were alternative and effective ways of keeping the spontaneous lysis within normal limits. Mechanical traumatization (centrifugation or shaking) caused considerably more damage to the red cells when these were highly concentrated than when they were diluted. A cell suspension in SAG is a more suitable product for hemotherapy than strongly packed red cell concentrates.

Red Cell Apheresis: New Concepts of Blood Component Processing

“…Figure 4 shows the direct comparison between the storage of a standard red cell concentrate (RCC-SAGM) and the apheresed collected RCC-SAGM for metabolical parameters. Glucose consumption and lactose accumulation correspond to storage data of erythro- ~cytes in that type of additive solution (11). After 14 days of storage, the intracellular ATP level and particularly the 2,3-DPG concentration have still values of 92% resp.…”

Evaluation of a whole‐blood WBC‐reduction filter that saves platelets: in vitro studies

“…The difference observed may be associated with the early removal of WBCs in the present study. Previous studies indicate that RBCs stored in the presence of WBCs undergo increased hemolysis, as protease discharged primarily from granulocytes at disintegration has a potent hemolytic effect 21 . We were not able to compare the extracellular potassium levels in filtered whole blood with those in conventionally prepared RBCs, because of a lack of data.…”

Ketoprofen-loaded pomegranate seed oil nanoemulsion stabilized by pullulan: Selective antiglioma formulation for intravenous administration

“…This higher hemolytic activity can be explained by the higher surface area of the nanocarriers, which improves the contact area with the erythrocytes, especially if the drug is associated more externally [35,36]. According to the literature, the accepted limits should vary between 1 and 5% for spontaneous hemolysis [37,38]. Therefore, 50 g/mL was not considered suitable for intravenous administration.…”