RED CELL, PLASMA AND BLOOD VOLUME IN HEALTHY MEN MEASURED BY RADIOCHROMIUM (Cr51) CELL TAGGING AND HEMATOCRIT: INFLUENCE OF AGE, SOMATOTYPE AND HABITS OF PHYSICAL ACTIVITY ON THE VARIANCE AFTER REGRESSION OF VOLUMES TO HEIGHT AND WEIGHT COMBINED*

Wennesland, Reidar; Em, Brown; Hopper, J.; Hodges, J. L.; Guttentag, Otto E.; Scott, Kenneth G.; Tucker, I. N.; Bradley, Barbara

doi:10.1172/jci103883

Cited by 193 publications

(72 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We wanted to determine a nomogram from which to predict the red blood cell, plasma and total blood volumes from body surface area and body weight. Red blood cell, plasma and total blood volumes were found to correlate with both body surface area (BSA) and body weight (BW), a finding similar to that reported by other investigators who studied younger subjects [1][2][3][4][5][6][7][8][9][10] . In our study, the regression equations were slightly more accurate for body surface area (BSA) than for body weight (BW).…”

supporting

confidence: 80%

Red Blood Cell Volume, Plasma Volume and Total Blood Volume in Healthy Elderly Men and Women Aged 64 to 100

Valeri¹,

Pivacek²,

Siebens³

et al. 1992

View full text Add to dashboard Cite

supporting

confidence: 80%

Red Blood Cell Volume, Plasma Volume and Total Blood Volume in Healthy Elderly Men and Women Aged 64 to 100

Valeri¹,

Pivacek²,

Siebens³

et al. 1992

View full text Add to dashboard Cite

“…The method' of labeling the autologous cells with NaCr5O4 and of determining their volume of distribution (VCr"1) was as described in the study on men (1,4). Tests were performed before breakfast or, rarely, in the afternoon when lunch had been omitted.…”

Section: Methodsmentioning

confidence: 99%

Red Cell, Plasma, and Blood Volume in Healthy Women Measured by Radiochromium Cell-Labeling and Hematocrit*

Em¹,

Hopper²,

Hodges³

et al. 1962

J. Clin. Invest.

Self Cite

190

View full text Add to dashboard Cite

In a previous study of 201 healthy men (1), red cell volume (Vrbc) was measured by a modification of Sterling and Gray's radiochromium method (2), and whole blood and plasma volumes (Vwb and Vpl) were derived from venous hemiatocrits. The influence of factors other than body size on the variance of the data was studied, and standards for predicting normal volumes were derived. The present report describes a similar examination of 101 women. SUBJECTS AND METHODSThe women, all of whom volunteered for study, were actively employed as housewives, laboratory personnel, nurses, or office workers, and most were white (Table I) tResearch Fellow, American Heart Association. prevent venous congestion during sampling. Hematocrit readings were not corrected for plasma trapping, and it was assumed that Vwb = VCr"1 (no allowance was made for a difference between the hematocrit of venous blood and that of the body as a whole) (1). Cell and plasma volumes were calculated by the formulas: Vrhc = VCr`1 X hematocrit; Vpl = VCr5`-Vrbc.The data were treated as outlined in the study of men (1). Regression equations were calculated by the method of least squares to describe the relations between volumes (Vrbc, Vpl, and Vwb) and body measurements (weight, height, weight and height combined, and surface area). The differences between the observed volumes (Vrbc and Vpl) of each woman and the mean volumes found in women of the same height and weight vere calculated by means of Equations 3 and 7, Table II. These differences, or "residuals," wsere used to analyze the influence of factors other than body size On the blood volumes. Table I summarizes the data. In Figure 1, each of the 101 subjects is represented according to her weight and height. In general, the relationship between weight and height was fairly uniform. Notable exceptions were four women weighing over 81 kg, who proved to be 23 to 58 per cent overweight for height and age according to actuarial tables (5). The weight: height ratios of these subjects were more than 2 standard deviations above the mean of the ratios of the other 97 women. For this reason and because the series included so few large women, regression equations describing the relationships of the volumes to body measurements were prepared only for the 97 women weighing less than 74 kg (Table II). RESULTS Volumes in relation to height and weightThe bivariate regression equations described straight lines and the trivariate equations, planes without curvature (see Figures 1 to 4). The location of the regression plane for Vrbc in rela-2182

show abstract

“…Three duplicate 1-mL samples of blood were measured in a g-sample changer (Packard), with an appropriate standard activity of 99m Tc to convert from counts to megabecquerels. The total amount of radioactivity in the blood was calculated using a total blood volume based on sex, individual body weight and height, and body surface area and expressed as a percentage of the injected dose of 99m Tc-HMPAO-labeled monocytes (7)(8)(9).…”

Section: Study Protocolmentioning

confidence: 99%

Biodistribution and Radiation Dosimetry of ^99mTc-HMPAO–Labeled Monocytes in Patients with Rheumatoid Arthritis

Bennink¹,

Thurlings²,

Hemert³

et al. 2008

J Nucl Med

View full text Add to dashboard Cite

Rheumatoid arthritis (RA) involves the accumulation of monocytederived macrophages in the affected synovial tissue. This process of cell migration could be portrayed scintigraphically to monitor noninvasively the effects of therapy on the progress of the disease. For this purpose, labeling of purified autologous monocytes with 99m Tc-hexamethylpropyleneamine oxime ( 99m Tc-HMPAO) at very high specific radioactivity has recently been developed. The aim of this study was to assess the biodistribution and radiation dosimetry of 99m Tc-HMPAO-labeled monocytes in adult patients with RA. Methods: In 8 patients with RA, monocytes were isolated from 100 mL of blood and labeled with 99m Tc-HMPAO to a yield of 10 Bq/cell. Multiple whole-body scans were performed up to 20 h after reinjection of an average of 200 MBq of 99m Tc-HMPAO-labeled monocytes. Urine and blood samples were collected. The fraction of administered activity in 7 source organs was quantified from the attenuation-corrected geometric mean counts in conjugate views. Radiation-absorbed doses were estimated with OLINDA/EXM software. Results: Autologous monocytes labeled with 99m Tc-HMPAO at high intracellular yields showed in vivo kinetics comparable with labeled leukocytes, with initial trapping in the lungs followed by distribution into the liver, spleen, and bone marrow. The radiation-absorbed estimates for 99m Tc-HMPAO-labeled monocytes were comparable with those for 99m Tc-HMPAOlabeled mixed white blood cells, with an effective dose of 0.011 mSv/MBq. Conclusion: 99m Tc-HMPAO-labeled monocytes have biodistribution and radiation dosimetry similar to those of 99m Tc-HMPAO-labeled mixed white blood cells and might therefore be used for in vivo monitoring of immunomodulating therapy in patients with RA.Key Words: joint/muscle; radiobiology/dosimetry; radiopharmaceuticals; biodistribution; monocytes; rheumatoid arthritis Nucl Med 2008; 49:1380 49: -1385 49: DOI: 10.2967 Rheumat oid arthritis (RA) is a chronic inflammatory disease affecting synovial tissue in multiple joints. Monocytederived macrophage cells accumulate in the inflamed synovium and thereby contribute to the perpetuation of inflammation and destruction of the affected joints (1). Reduced migration of inflammatory cells such as monocytes or macrophages into the synovial compartment or a decrease in the retention of these cells at the site of inflammation may be attained by treatment of patients with antibodies against tumor necrosis factor-a (TNF-a) (2) or perhaps with chemokine receptor antagonists (3). JFor noninvasive monitoring of treatment, migration of cells toward sites of inflammation in vivo can be portrayed scintigraphically after radiolabeling of the cells in vitro with 99m Tc-labeled hexamethylpropyleneamine oxime (HMPAO). Therefore, labeling of purified autologous monocytes with 99m Tc-HMPAO at very high specific radioactivity has recently been developed (4).In this study, the biodistribution and radiation dosimetry of 99m Tc-HMPAO-labeled monocytes were assessed in adult patients w...

show abstract

RED CELL, PLASMA AND BLOOD VOLUME IN HEALTHY MEN MEASURED BY RADIOCHROMIUM (Cr51) CELL TAGGING AND HEMATOCRIT: INFLUENCE OF AGE, SOMATOTYPE AND HABITS OF PHYSICAL ACTIVITY ON THE VARIANCE AFTER REGRESSION OF VOLUMES TO HEIGHT AND WEIGHT COMBINED*

Cited by 193 publications

References 25 publications

Red Blood Cell Volume, Plasma Volume and Total Blood Volume in Healthy Elderly Men and Women Aged 64 to 100

Red Blood Cell Volume, Plasma Volume and Total Blood Volume in Healthy Elderly Men and Women Aged 64 to 100

Red Cell, Plasma, and Blood Volume in Healthy Women Measured by Radiochromium Cell-Labeling and Hematocrit*

Biodistribution and Radiation Dosimetry of ^99mTc-HMPAO–Labeled Monocytes in Patients with Rheumatoid Arthritis

Contact Info

Product

Resources

About

RED CELL, PLASMA AND BLOOD VOLUME IN HEALTHY MEN MEASURED BY RADIOCHROMIUM (Cr51) CELL TAGGING AND HEMATOCRIT: INFLUENCE OF AGE, SOMATOTYPE AND HABITS OF PHYSICAL ACTIVITY ON THE VARIANCE AFTER REGRESSION OF VOLUMES TO HEIGHT AND WEIGHT COMBINED*

Cited by 193 publications

References 25 publications

Red Blood Cell Volume, Plasma Volume and Total Blood Volume in Healthy Elderly Men and Women Aged 64 to 100

Red Blood Cell Volume, Plasma Volume and Total Blood Volume in Healthy Elderly Men and Women Aged 64 to 100

Red Cell, Plasma, and Blood Volume in Healthy Women Measured by Radiochromium Cell-Labeling and Hematocrit*

Biodistribution and Radiation Dosimetry of 99mTc-HMPAO–Labeled Monocytes in Patients with Rheumatoid Arthritis

Contact Info

Product

Resources

About

Biodistribution and Radiation Dosimetry of ^99mTc-HMPAO–Labeled Monocytes in Patients with Rheumatoid Arthritis