Red cell genotyping in Thailand: trends for blood transfusion in Asian populations

Nathalang, Oytip

doi:10.1111/voxs.12547

Cited by 3 publications

(4 citation statements)

References 19 publications

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“…The DI*A allele (predicted Di a antigen) was significantly lower among southern Thais, while the JK*01 allele (predicted Jk a antigen) and KEL*01 allele (predicted K antigen) were significantly higher among southern Thais than among central and northern Thais. (19) However, the Mi a frequencies have not yet been reported in southern Thailand. This study constitutes the first to identify the predicted Mi a frequencies in Thai Muslim populations, which have their own religion, culture and sustainable lifestyle.…”

Section: Discussionmentioning

confidence: 99%

Frequencies of Predicted Mia Antigen Among Southern Thai Blood Donors

Khosidworachet

Mitundee

Intharanut

et al. 2022

J Southeast Asian Med Res

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Background: The Mia antigen (MNS7) of the MNS blood group system is clinically important in Asian populations. Anti-Mia has been implicated in hemolytic transfusion reactions and hemolytic disease of the fetus and newborn in Thai populations. However, data of this antigen frequency among southern Thais remains unknown. Objective: This study aimed to determine and predict Mia antigen frequencies among southern Thai blood donors and to estimate the risk of alloimmunization among Thais. Methods: A cross-sectional study was conducted. Altogether, 400 southern and 500 central Thai blood samples were genotyped for GYP(B-A-B) and GYP(A-B-A) MNS hybrids using polymerase chain reaction with sequence-specific primer (PCR-SSP). Results: Among them, 19 of 400 (4.45%), and 28 of 500 (9.33%) were positive with the set of GP. Hut, GP.HF, GP.Mur, GP.Hop, and GP.Bun. No GP.Vw phenotype was found among southern and central Thais. The predicted Mi(a+)frequency among southern Thais was significantly lower than among central and northern Thais (p<0.05). Its frequency was similar to Vietnamese, Taiwanese, and Southern Han Chinese populations (p>0.05) but significantly differed from Indonesian, Filipino, and Chinese (Guangzhou) populations (p<0.05). The risk of Mia alloimmunization among southern Thais was significantly lower than among both Thai groups (p<0.05). Conclusion: This constitutes the first study to report Mi(a+) frequencies among southern Thais, supporting the estimation risk of alloimmunization and providing transfusion safety among Thai populations.

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Section: Discussionmentioning

confidence: 99%

Frequencies of Predicted Mia Antigen Among Southern Thai Blood Donors

Khosidworachet

Mitundee

Intharanut

et al. 2022

J Southeast Asian Med Res

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“… 18 Genotyping methods such as PCR-SSP and HRM are applicable to predict the phenotypes of extended blood group systems. 14 , 15 , 19 …”

Section: Discussionmentioning

confidence: 99%

“…In Thailand, low-throughput methods such as PCR-SSP and multiplex PCR were performed to predict extended blood group phenotypes in donor and patient populations, and the results were consistent with the serological testing. 14 Recently, high-resolution melting curve (HRM) analysis has been implemented to reduce errors in interpretation and manual inspection for red cell genotyping using PCR-SSP. 15 Therefore, this study aimed to develop genotyping methods to predict Co a and Co b antigens and to estimate transfusion-induced alloimmunization risks in three Thai blood donor populations.…”

Section: Introductionmentioning

confidence: 99%

Genotyping Approach to Predict Coa and Cob Antigens in Thai Blood Donor Populations

Nathalang¹,

Asisathaporn²,

Intharanut³

et al. 2023

JBM

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Purpose Co a and Co b antigens of the Colton (CO) blood group system are implicated in acute and delayed hemolytic transfusion reactions (HTRs). Owing to the inadequate supply of specific antiserum, data on CO phenotypes remain limited. This study aimed to develop genotyping methods to predict Co a and Co b antigens and to estimate transfusion-induced alloimmunization risks in three Thai blood donor populations. Materials and Methods The study included 2451 blood samples from unrelated healthy Thai blood donors obtained from central, northern, and southern Thailand. DNA sequencing was used to determine the CO*A and CO*B alleles. In-house PCR with sequence-specific primers (PCR-SSP) and high-resolution melting curve (HRM) assays were performed and genotyping results were compared using DNA sequencing. CO*A and CO*B allele frequencies among Thais were determined using PCR-SSP and their frequencies were compared with other populations. The risks of Co a and Co b transfusion-induced alloimmunization among Thai donor populations were calculated. Results The validated genotyping results by PCR-SSP and HRM assays agreed with DNA sequencing. The CO*A / CO*A was the most common (100.0, 100.0, and 99.3%), followed by CO*A / CO*B (0.0, 0.0, and 0.7%) among central, northern and southern Thais. Homozygous CO*B / CO*B was not found. The CO*A and CO*B allele frequencies among central Thais significantly differed compared among southern Thais ( p < 0.01) but not among northern Thais. Those allele frequencies among Thais were similar to those of Taiwanese, Chinese and Malay-Malaysian populations but not to South Asian, Southeast Asian, Korean, Japanese, Filipino, French Basque, and Maltese populations ( p < 0.01). A higher risk of anti-Co b production rather than anti-Co a production was particularly noted in the southern Thai population. Conclusion This study constitutes the first to determine CO*A and CO*B genotypes using PCR-SSP and HRM assays among Thais and this finding would be beneficial in predicting alloimmunization risk and providing safe transfusions among Thais.

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“…In Thailand, the allele-specific PCR or PCR sequencespecific primer (PCR-SSP) as well as multiplex PCR were applied in donor and patient populations to predict antigens in several blood group systems including Kidd (JK*01 and JK*02), Duffy (FY*01, FY*02 and FY*02 N.01), Diego (DI*01 and DI*02), MNS (GYPB*03 and GYPB*04), Kell (KEL*01 and KEL*02), Rh (RHCE*E and RHCE*e) and Dombrock (DO*01 and DO*02) [9][10][11][12][13][14][15]. Commercially available high-throughput techniques for red cell genotyping have also been implemented, but only in specialised reference laboratories due to their high cost of reagents and sophisticated equipment required [16].…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Melting Curve Analysis to Predict Extended Blood Group Phenotypes among Thai Donors and Patients

Nathalang

Intharanut

Chidtrakoon

2021

Transfus Med Hemother

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Background: High-resolution melting (HRM) analysis is an alternative method for red cell genotyping. Differences in melting curves between homozygous and heterozygous genotypes can predict phenotypes in blood group systems based on single-nucleotide polymorphisms. This study aimed to implement HRM analysis to predict additional extended blood group phenotypes in Thai donor and patient populations. Methods: Blood samples obtained from 300 unrelated Thai blood donors and 23 patients with chronic transfusions were included. HRM analysis was developed and validated in genotyping of KEL*01 and KEL*02, JK*01 and JK*02, FY*01, FY*02, and FY*02 N.01, DI*01 and DI*02, GYPB*03 and GYPB*04, RHCE*E and RHCE*e, and DO*01 and DO*02. Then genotyping results from HRM and polymerase chain reaction with sequence-specific primer (PCR-SSP) and phenotyping results were compared. Results: The validated genotyping results in known DNA controls by HRM analysis agreed with DNA sequencing. The genotyping results among 300 donors in 15 alleles by HRM analysis were in complete concordance with those obtained by serological testing and PCR-SSP. The sensitivity and specificity of the HRM assay were both 100%. Among patients, 13 had alloantibodies that possessed predicted antigen-negative phenotypes corresponding to those antibody specificities, and the highest probability of genotyped-matched donors was given to the remaining patients. Conclusions: We developed and implemented the HRM analysis assay for red cell genotyping to predict extended blood group antigens in Thai donor and patient populations. The data from this study may help inform about and support transfusion care of Thai patients to reduce the risk of alloimmunisation.

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Red cell genotyping in Thailand: trends for blood transfusion in Asian populations

Cited by 3 publications

References 19 publications

Frequencies of Predicted Mia Antigen Among Southern Thai Blood Donors

Frequencies of Predicted Mia Antigen Among Southern Thai Blood Donors

Genotyping Approach to Predict Coa and Cob Antigens in Thai Blood Donor Populations

High-Resolution Melting Curve Analysis to Predict Extended Blood Group Phenotypes among Thai Donors and Patients

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