Red blood cells: The primary reservoir of macrophage migration inhibitory factor in whole blood

Karsten, Elisabeth; Hill, Charles; Herbert, Ben R.

doi:10.1016/j.cyto.2017.12.005

Cited by 22 publications

(17 citation statements)

References 35 publications

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“…CXCL12 is the typical CXCR4 ligand, but this receptor can also be activated by MIF, which functions as a non-cognate ligand [38]. Moreover, it has been demonstrated that red blood cells are an important source of MIF, contributing with~99% of total MIF content in blood [39]. In fact, the cell-permeable MIF antagonist, ISO 1, was able to inhibit NET release in response to infected erythrocytes ( Fig 4B).…”

Section: Net Production In Response To Infected Rbcs Is Triggered By mentioning

confidence: 94%

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CXCR4 and MIF are required for neutrophil extracellular trap release triggered by Plasmodium-infected erythrocytes

et al. 2020

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Neutrophil extracellular traps (NETs) evolved as a unique effector mechanism contributing to resistance against infection that can also promote tissue damage in inflammatory conditions. Malaria infection can trigger NET release, but the mechanisms and consequences of NET formation in this context remain poorly characterized. Here we show that patients suffering from severe malaria had increased amounts of circulating DNA and increased neutrophil elastase (NE) levels in plasma. We used cultured erythrocytes and isolated human neutrophils to show that Plasmodium-infected red blood cells release macrophage migration inhibitory factor (MIF), which in turn caused NET formation by neutrophils in a mechanism dependent on the C-X-C chemokine receptor type 4 (CXCR4). NET production was dependent on histone citrullination by peptidyl arginine deiminase-4 (PAD4) and independent of reactive oxygen species (ROS), myeloperoxidase (MPO) or NE. In vitro, NETs functioned to restrain parasite dissemination in a mechanism dependent on MPO and NE activities. Finally, C57/B6 mice infected with P. berghei ANKA, a well-established model of cerebral malaria, presented high amounts of circulating DNA, while treatment with DNAse increased parasitemia and accelerated mortality, indicating a role for NETs in resistance against Plasmodium infection.

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Section: Net Production In Response To Infected Rbcs Is Triggered By mentioning

confidence: 94%

“…We observed that MIF, acting through CXCR4, were required to NET release induced by infected red blood cells. Recent evidences suggest that red blood cells are a major source of MIF in the bloodstream [39]. The mechanism by which MIF is released from infected erythrocytes is not well characterized.…”

Section: Plos Pathogensmentioning

confidence: 99%

CXCR4 and MIF are required for neutrophil extracellular trap release triggered by Plasmodium-infected erythrocytes

et al. 2020

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“…MIF concentration in whole blood is 1000-fold increased than in plasma. Since leukocytes and platelets have been shown to minimally contribute to MIF concentration, RBCs represent the major reservoir of this factor [91], which is also functionally active [91].…”

Section: Red Blood Cellsmentioning

confidence: 99%

Platelet Concentrates in Musculoskeletal Medicine

Mariani

Pulsatelli

2020

IJMS

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Platelet concentrates (PCs), mostly represented by platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are autologous biological blood-derived products that may combine plasma/platelet-derived bioactive components, together with fibrin-forming protein able to create a natural three-dimensional scaffold. These types of products are safely used in clinical applications due to the autologous-derived source and the minimally invasive application procedure. In this narrative review, we focus on three main topics concerning the use of platelet concentrate for treating musculoskeletal conditions: (a) the different procedures to prepare PCs, (b) the composition of PCs that is related to the type of methodological procedure adopted and (c) the clinical application in musculoskeletal medicine, efficacy and main limits of the different studies.

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“…This phenomenon is known to contribute to vascular damage or microcirculatory blood flow irregularities [24]. Another significant consequence of PFH is the release of macrophage migration inhibitory factor (MIF), since RBCs contain large concentrations of this enzymatically and chemotactically active cytokine [25]. MIF is identified as a very potent inflammatory cytokine.…”

Section: Introductionmentioning

confidence: 99%

Assessing clinical implications and perspectives of the pathophysiological effects of erythrocytes and plasma free hemoglobin in autologous biologics for use in musculoskeletal regenerative medicine therapies. A review

Everts

Malanga

Paul

et al. 2019

Regenerative Therapy

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Autologous biologics, defined as platelet-rich plasma (PRP) and bone marrow aspirate concentrate (BMC), are cell-based therapy treatment options in regenerative medicine practices, and have been increasingly used in orthopedics, sports medicine, and spinal disorders. These biological products are produced at point-of-care; thereby, avoiding expensive and cumbersome culturing and expansion techniques. Numerous commercial PRP and BMC systems are available but reports and knowledge of bio-cellular formulations produced by these systems are limited. This limited information hinders evaluating clinical and research outcomes and thus making conclusions about their biological effectiveness. Some of their important cellular and protein properties have not been characterized, which is critical for understanding the mechanisms of actions involved in tissue regenerative processes. The presence and role of red blood cells (RBCs) in any biologic has not been addressed extensively. Furthermore, some of the pathophysiological effects and phenomena related to RBCs have not been studied. A lack of a complete understanding of all of the biological components and their functional consequences hampers the development of clinical standards for any biological preparation. This paper aims to review the clinical implications and pathophysiological effects of RBCs in PRP and BMC; emphasizes hemolysis, eryptosis, and the release of macrophage inhibitory factor; and explains several effects on the microenvironment, such as inflammation, oxidative stress, vasoconstriction, and impaired cell metabolism.

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Red blood cells: The primary reservoir of macrophage migration inhibitory factor in whole blood

Cited by 22 publications

References 35 publications

CXCR4 and MIF are required for neutrophil extracellular trap release triggered by Plasmodium-infected erythrocytes

CXCR4 and MIF are required for neutrophil extracellular trap release triggered by Plasmodium-infected erythrocytes

Platelet Concentrates in Musculoskeletal Medicine

Assessing clinical implications and perspectives of the pathophysiological effects of erythrocytes and plasma free hemoglobin in autologous biologics for use in musculoskeletal regenerative medicine therapies. A review

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