Red blood cells stimulate fibroblast-mediated contraction of three dimensional collagen gels in co-culture

Fredriksson, Karin; Lundahl, Joachim; Fernvik, Eva; Liu, Xiangde; Rennard, Stephen I.; Sköld, C. Magnus

doi:10.1007/pl00000300

Cited by 9 publications

(18 citation statements)

References 26 publications

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“…In the current study, contraction over time was suppressed in samples with physiologic and higher erythrocyte concentrations over 21 days. This is in contrast to another study by Fredriksson et al17 which showed an increase in contraction in fibroblast‐containing collagen gels with addition of erythrocytes in a time and concentration‐dependent manner. Significant increase was shown compared to gels without erythrocytes on all days between 1 and 5, and the augmentation was dependent on the number of erythrocytes added.…”

Section: Discussioncontrasting

confidence: 99%

Erythrocytes inhibit ligament fibroblast proliferation in a collagen scaffold

Harrison

Vavken

Murray

2011

Journal Orthopaedic Research

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In this work, we hypothesized that the concentration of erythrocytes in a provisional scaffold would have a significant effect on three of the major biological processes occurring in early wound healing. ACL fibroblast proliferation, collagen production, and scaffold contraction were measured in collagen gels containing fibroblasts and erythrocytes in subphysiologic (1 × 108 erythrocytes/ml), physiologic (1 × 109 erythrocytes/ml), and supraphysiologic (1 × 1010 erythrocytes/ml) concentrations. Fibroblast-seeded gels containing only platelet-poor plasma were used as a control group. All gels were cultured for 1, 14, and 21 days. DNA, ELISA for procollagen and scaffold size measurements were used to quantify the three above parameters of wound healing. Samples with concentrations of erythryocytes lower than that in whole blood stimulated greater fibroblast proliferation and scaffold contraction than those with erythrocyte concentrations similar to that in whole blood (p <0.027; p <0.03). Increasing the erythrocyte concentration over that in the whole blood stimulated fibroblast collagen production (p <0.009) and limited scaffold contraction (p <0.031). Further work examining the role of the erythrocyte in the early provisional scaffold is warranted.

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Section: Discussioncontrasting

confidence: 99%

Erythrocytes inhibit ligament fibroblast proliferation in a collagen scaffold

Harrison

Vavken

Murray

2011

Journal Orthopaedic Research

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“…These mediators can also be released from the RBC, indicating a role for RBC in the regulation of inflammation (8,9,12,33,53). In line with these data, we have recently demonstrated that RBC can stimulate fibroblast-mediated collagen gel contraction in a time-and concentration-dependent manner and that coculture results in increased release of fibronectin (16). We also showed that RBC can stimulate fibroblast secretion of IL-8 and increase neutrophil migration in vitro (19).…”

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confidence: 68%

“…Others have shown that RBC can induce fibrosis in an animal model (27), and in previous studies we have demonstrated that both whole RBC and cell-free RBC-CM have the capacity to stimulate fibroblast-mediated collagen gel contraction (16) and fibroblast secretion of IL-8 (19). In the present study we show that both RBC and RBC-CM can induce MMP secretion and activity from human lung fibroblasts, indicating a role of intact RBC as well as soluble factors released.…”

Section: Discussionmentioning

confidence: 89%

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Red blood cells increase secretion of matrix metalloproteinases from human lung fibroblasts in vitro

Fredriksson

Liu

Lundahl

et al. 2006

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…[15][16][17][18][19][20][21] Fibroblasts naturally contract the extracellular matrix (ECM) to close a wound, and, when placed in a collagen gel, respond in a similar fashion and contract the gel. Another in vitro model of airway involves culturing epithelial cells as monolayer on a membrane and fibroblasts as a monolayer a fixed distance away separated by culture medium.…”

Section: )mentioning

confidence: 99%

Viable Alternatives to in vivo Tests for Evaluating the Toxicity of Engineered Carbon Nanotubes

Kwon¹,

Eo²

2012

Korean Journal of Environmental Health Sciences

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Carbon nanotubes (CNTs) stand at the frontier of nanotechnology and are destined to stimulate the next industrial revolution. Rapid increase in their production and use in the technology industry have led to concerns over the effects of CNT on human health and the environment. The prominent use of CNTs in biomedical applications also increases the possibility of human exposure, while properties such as their high aspect ratio (fiber-like shape) and large surface area raise safety concerns for human health if exposure does occur. It is crucial to develop viable alternatives to in vivo tests in order to evaluate the toxicity of engineered CNTs and develop validated experimental models capable of identifying CNTs' toxic effects and predicting their level of toxicity in the human respiratory system. Human lung epithelial cells serve as a barrier at the interface between the surrounding air and lung tissues in response to exogenous particles such as air-pollutants, including CNTs. Monolayer culture of the key individual cell types has provided abundant fundamental information on the response of these cells to external perturbations. However, such systems are limited by the absence of cell-cell interactions and their dynamic nature, which are both present in vivo. In this review, we suggested two viable alternatives to in vivo tests to evaluate the health risk of human exposure to CNTs.

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Red blood cells stimulate fibroblast-mediated contraction of three dimensional collagen gels in co-culture

Abstract: The present study shows that RBC can interact with mesenchymal cells in vitro. The ability of RBC to modulate fibroblast-mediated contraction in vitro, might therefore be an important mechanism regulating repair processes after injury.

Cited by 9 publications

References 26 publications

Erythrocytes inhibit ligament fibroblast proliferation in a collagen scaffold

Erythrocytes inhibit ligament fibroblast proliferation in a collagen scaffold

Red blood cells increase secretion of matrix metalloproteinases from human lung fibroblasts in vitro

Viable Alternatives to in vivo Tests for Evaluating the Toxicity of Engineered Carbon Nanotubes

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