Red blood cells labelling with ^99mTc‐d,l‐HMPAO: an alternative method for specific cases

Abstract

SummaryThe effectiveness of the classic methods for red blood cells (RBCs) labelling with 99m Tc has been demonstrated in nuclear medicine. However, nuclear physicians have found, in certain circumstances, this diagnostic technique fails and poor quality images are obtained. In this work we report on an alternative method that is of useful in these occasions, for in vitro labelling RBCs with 99m Tc-d,l-HMPAO complex. The study shows a high and reproducible labelling efficiency (94.14 AE 0.38), using low amount of tin. The RBCs were isolated from plasma and other interfering blood cells before adding 99m Tc-d, l-HMPAO. The tracer was retained and the elution rate from RBCs was low (less than 6% after 120 min). The preclinical results indicate that this new method could be a good alternative to the standard classic methods for specific cases.

Marcaje de leucocitos con ^99mTc-exametazima y policitemia vera: un hallazgo casual

“…En el presente caso no se produjo sedimentación de los hematíes, a pesar de prolongar el proceso durante 120 minutos. Al no poder separar los hematíes de los leucocitos por el procedimiento recomendado, el complejo 99m Tc-hexametazima marcaría también los hematíes haciendo que las imágenes no fueran resolutivas (9) . Ante la imposibilidad de realizar la prueba, se programó un estudio con anticuerpos antigranulocitos marcados con 99m Tc que no mostró anomalías en la fijación del radiofármaco indicativas de un proceso infeccioso (Figura 2).…”