Red Blood Cells Elicit Platelet-Dependent Neutrophil Recruitment Into Lung Airspaces

Abstract

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Single and Multiplex Immunohistochemistry to Detect Platelets and Neutrophils in Rat and Porcine Tissues

“…However, surprisingly, anti-mouse antibodies recognising CD42b and CD49b did not adequately stain platelets in rat tissues, so we have still to identify a suitable platelet antibody for use in FFPE rat tissue. However, during the protocol optimisation period, another laboratory reported rat platelet staining using an anti-CD61 antibody for immunofluorescence microscopy [ 29 ]. Whilst this antibody was also successful in staining for platelets in frozen rat tissue in a blast-injury model, it was not suitable for use in FFPE tissue, despite testing several different antigen retrieval conditions.…”

Investigating and imaging platelets in inflammation

“…In addition to aggregating at sites of trauma, it is now clear that platelets can roll on and become adhesive as singlets to the endothelium of inflamed blood vessels (Cleary et al, 2019;Shah et al, 2021). Platelets also interact with leukocytes, assisting in leukocyte activation and recruitment (Zarbock et al, 2006;Looney et al, 2009;Sreeramkumar et al, 2014;Pan et al, 2015;Rossaint et al, 2016;Zuchtriegel et al, 2016;Arnold et al, 2021), and promoting release of neutrophil extracellular traps (Clark et al, 2007;Caudrillier et al, 2012;Valet et al, 2022). Additionally, platelets act to seal breaches created by transmigrating leukocytes to prevent inflammatory bleeding (Gros et al, 2015;Rayes et al, 2018;Nicolai et al, 2020;Wu et al, 2021;Kaiser et al, 2022), migrate to collect and kill pathogens (Gaertner et al, 2017;Amison et al, 2018b), modulate adaptive immune responses (Amison et al,2 2018a; Wu et al, 2021), and respond to antibodies, complement activation, allergens and virulence factors (Joseph et al, 1983;Beutier et al, 2018;Cleary et al, 2020;Wu et al, 2021).…”

What's New in Shock, August 2021?

“…The next article describes lung inflammation after blast injury and the association of red blood cells with infiltration of leukocytes and platelets. Arnold et al (14) show that red cells (whole or lysed) infused into the lungs of mice elicited an elevation in inflammatory cells. Whole red cells elicited a greater response in macrophages, lysed red cells elicited a greater response in neutrophils and neutrophil–platelet complexes.…”