Red blood cells are dynamic reservoirs of cytokines

Karsten, Elisabeth; Breen, Edmond J.; Herbert, Ben R.

doi:10.1038/s41598-018-21387-w

Cited by 92 publications

(82 citation statements)

References 41 publications

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“…In addition, INF-γ contributes to apoptosis of the erythroid progenitors and decrease the EPO receptor expression [17]. 3) RBC are dynamic reservoirs of cytokines [18]. Decreased deformability of RBC in severe illness leads to RBC lysis and release of intracellular contents into the circulation [19], including some inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

A Tool to Early Predict Severe Corona Virus Disease 2019 (COVID-19) : A Multicenter Study using the Risk Nomogram in Wuhan and Guangdong, China

Gong

Qiu

et al. 2020

Preprint

128

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Older age; higher LDH, CRP, RDW, DBIL, BUN; lower ALB on admission correlated with higher odds of severe COVID-19. An effective prognostic nomogram composed of 7 features could allow early identification of patients at risk of exacerbation to severe COVID-19. Abstract BackgroundDue to no reliable risk stratification tool for severe corona virus disease 2019 patients at admission, we aimed to construct an effective model for early identifying cases at high risk of progression to severe COVID-19. MethodsIn this retrospective three-centers study, 372 non-severe COVID-19 patients during hospitalization were followed for more than 15 days after admission. Patients who deteriorated to severe or critical COVID-19 and patients who kept non-severe state were assigned to the severe and non-severe group, respectively. Based on baseline data of the two groups, we constructed a risk prediction nomogram for severe COVID-19 and evaluate its performance. ResultsThe train cohort consisted of 189 patients, while the two independent validation cohorts consisted of 165 and 18 patients. Among all cases, 72 (19.35%) patients developed severe COVID-19. We found that old age, and higher serum lactate dehydrogenase, C-reactive protein, the coefficient of variation of red blood cell distribution width, blood urea nitrogen, direct bilirubin, lower albumin, are associated with severe COVID-19. We generated the nomogram for early identifying severe COVID-19 in the train cohort (AUC 0.912 [95% CI 0.846-0.978], sensitivity 85.71%, specificity 87.58%); in validation cohort (0.853 [0.790-0.916], 77.5%, 78.4%). The calibration curve for probability of severe COVID-19 showed optimal agreement between prediction by nomogram and actual observation. Decision curve and clinical impact curve analysis indicated that nomogram conferred high clinical net benefit. ConclusionOur nomogram could help clinicians to early identify patients who will exacerbate to severe COVID-19, which will enable better centralized management and early treatment of severe patients.

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Section: Discussionmentioning

confidence: 99%

A Tool to Early Predict Severe Corona Virus Disease 2019 (COVID-19) : A Multicenter Study using the Risk Nomogram in Wuhan and Guangdong, China

Gong

Qiu

et al. 2020

Preprint

128

160

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“…These cytokines are all highly potent stimulators of the immune system and the endothelium and could be responsible for part of immune modulation effects seen in some patients post‐transfusion . In addition, a recent study showed that RBCs harbour a number of cytokines which are released upon haemolysis . This could explain the increase in cytokine concentration in RCC supernatants during HS resulting from increased RBC breakdown .…”

Section: Discussionmentioning

confidence: 99%

Red cell supernatant effects on endothelial cell function and innate immune activation is influenced by donor age and sex

Kipkeu

Almizraq

Branch

et al. 2018

ISBT Science Series

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Background and objectives Blood donor characteristics significantly affect the pre-storage and post-storage quality of red cell concentrates (RCCs). This study investigated the impact of donor age and sex on RCC characteristics and the effect of RCC supernatants on the vascular endothelium and monocytes.Materials and methods RCC units selected from four donor groups based on donor age and sex (male ≤ 30-years old (yo), male ≥ 60-yo, female ≤ 30-yo and female ≥ 60-yo) were stored at 1-6°C and tested on day 7, 21 and 42. RCC supernatants were analysed for the number, concentration and size of extracellular vesicles (EVs). Human vein endothelial cells (HUVECs) monolayers were incubated with RCC supernatants and assessed for expression of vascular cell adhesion molecule-1 (VCAM-1) and E-selectin by flow cytometry as well as endothelial permeability using a trans-epithelial-electrical-resistance assay. Monocytes and HUVECs were incubated with RCC supernatants and supernatants assayed for cytokines/chemokines. ResultsRCCs from female donors were found to have lower unit volume, haemoglobin content and haematocrit compared to RCCs from male donors (P < 0Á05). RCC supernatants from female ≥ 60-yo had a higher concentration of large EVs (≥200 nm) compared to both male ≤ 30-yo (P = 0Á039) and male ≥ 60-yo supernatants (P = 0Á019). Treatment with RCC supernatants from male ≤ 30-yo donors induced lower expression of VCAM-1, lower cytokine/chemokine release and showed the least effect on endothelial permeability.Conclusion Donor characteristics affect RCC quality parameters and influence the endothelial and immune modulation potential of RCC supernatants in vitro.

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“…SCD is characterized by a pro-inflammatory state leading to high plasma cytokines levels. Karsten et al (101) recently showed that 46 cytokines can be detected in RBCs lysates of healthy individuals, and their median concentrations in RBCs were 12-fold higher than in plasma. Among them, the authors reported the presence of IFN-γ, IL-1β, IL-18, TNFα as well as several chemokines, such as IL-8 and RANTES.…”

Section: Inflammation and Rbcsmentioning

confidence: 99%