Red blood cell storage and cell morphology

Blasi, Barbara; D’Alessandro, Angelo; Ramundo, N.; Zolla, Lello

doi:10.1111/j.1365-3148.2012.01139.x

Cited by 147 publications

(176 citation statements)

References 33 publications

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“…Previous optical imaging studies of RBC morphology have required breaching the storage bag; these efforts, like those described above, found irreversible changes to the morphology with increasing storage duration [13][14][15]. Nonlinear optical imaging of RBCs has been accomplished via several different methods including two-photon absorption (TPA) [2,16], TPEF [3,17], and THG [18,19].…”

Section: Introductionmentioning

confidence: 99%

Multiphoton excited hemoglobin fluorescence and third harmonic generation for non-invasive microscopy of stored blood

Saytashev¹,

Glenn²,

Murashova³

et al. 2016

Biomed. Opt. Express

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Abstract:Red blood cells (RBC) in two-photon excited fluorescence (TPEF) microscopy usually appear as dark disks because of their low fluorescent signal. Here we use 15fs 800nm pulses for TPEF, 45fs 1060nm pulses for three-photon excited fluorescence, and third harmonic generation (THG) imaging. We find sufficient fluorescent signal that we attribute to hemoglobin fluorescence after comparing time and wavelength resolved spectra of other expected RBC endogenous fluorophores: NADH, FAD, biliverdin, and bilirubin. We find that both TPEF and THG microscopy can be used to examine erythrocyte morphology noninvasively without breaching a blood storage bag. Phys. Lett. 208(3-4), 276-282 (1993). 38. B. Kierdaszuk, I. Gryczynski, A. Modrak-Wojcik, A. Bzowska, D. Shugar, and J. R. Lakowicz, "Fluorescence of tyrosine and tryptophan in proteins using one-and two-photon excitation," Photochem. Photobiol. 61(4), 319-324 (1995). 39. S. Yoshida, T. Iizuka, T. Nozawa, and M. Hatano, "Studies on the charge transfer band in high spin state of ferric myoglobin and hemoglobin by low temperature optical and magnetic circular dichroism spectroscopy," Biochim. Biophys. Acta 405(1), 122-135 (1975).

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Section: Introductionmentioning

confidence: 99%

Multiphoton excited hemoglobin fluorescence and third harmonic generation for non-invasive microscopy of stored blood

Saytashev¹,

Glenn²,

Murashova³

et al. 2016

Biomed. Opt. Express

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“…With even longer storage times, the RBCs undergo further, irreversible damage to the membrane and cytoskeleton, and vesiculation occurs, which reduce the area-to-volume ratio and alter the cell shape to spherical and spinous. 32 Therefore, in 14d group, the RBCs were smaller and more hypodeformable. The morphology distributions of the stored RBC agree with the DI and could visually distinguish the RBCs with different storage times.…”

Section: B Explanation Of the Results And Comparison To The Literaturementioning

confidence: 99%

The mechanical properties of stored red blood cells measured by a convenient microfluidic approach combining with mathematic model

et al. 2016

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The mechanical properties of red blood cells (RBCs) are critical to the rheological and hemodynamic behavior of blood. Although measurements of the mechanical properties of RBCs have been studied for many years, the existing methods, such as ektacytometry, micropipette aspiration, and microfluidic approaches, still have limitations. Mechanical changes to RBCs during storage play an important role in transfusions, and so need to be evaluated pre-transfusion, which demands a convenient and rapid detection method. We present a microfluidic approach that focuses on the mechanical properties of single cell under physiological shear flow and does not require any high-end equipment, like a high-speed camera. Using this method, the images of stretched RBCs under physical shear can be obtained. The subsequent analysis, combined with mathematic models, gives the deformability distribution, the morphology distribution, the normalized curvature, and the Young's modulus (E) of the stored RBCs. The deformability index and the morphology distribution show that the deformability of RBCs decreases significantly with storage time. The normalized curvature, which is defined as the curvature of the cell tail during stretching in flow, suggests that the surface charge of the stored RBCs decreases significantly. According to the mathematic model, which derives from the relation between shear stress and the adherent cells' extension ratio, the Young's moduli of the stored RBCs are also calculated and show significant increase with storage. Therefore, the present method is capable of representing the mechanical properties and can distinguish the mechanical changes of the RBCs during storage. The advantages of this method are the small sample needed, high-throughput, and easy-use, which make it promising for the quality monitoring of RBCs. V C 2016 AIP Publishing LLC.

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“…The hemolysis level (% hemolysis) was calculated according to the following formula: % Hemolysis =100*(ODT-ODC)/ (ODTotal-ODC) where the ODC denotes OD of supernatant obtained for control (no stress) erythrocytes, ODT denotes OD of supernatant of erythrocytes subjected to oxidative stress and ODTotal correspond to the hemolysis obtained by conventional incubation (10 min) of erythrocytes in water [21]. Fig.…”

Section: Resultsmentioning

confidence: 99%

Effect of Free Radical Generating System- Adenosine Triphosphate/Ferrous Sulphate/Hydrogen Peroxide on Human Erythrocytes

Punjabi¹,

Gokhale²

2017

ijcrr

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Abstract:Erythrocytes constitute the major cell fraction of blood in the body continuously exposed to oxygen in vivo and hence liable to undergo oxidative damage like lipid oxidation and protein denaturation thereby affecting their function. The aim of the present work is to create an analogous oxidative environment for human erythrocytes in vitro using free radical generating system-ATP/FeSO4/H2O2 and to study its effect on their properties. It has been found that erythrocytes undergo hemolysis during exposure to oxidative stress at the same time cells become resistant to osmotic fragility after treatment. The membranes isolated from treated cells show additional bands corresponding to cytosolic proteins on electrophoretic gel. Decreased erythrocyte superoxide dismutase and catalase levels were observed in the cells exposed to ATP/FeSO4/H2O2. It is concluded that excessive oxidative environment results in alterations of the cellular systems.Keywords: erythrocyte; oxidative stress; adenosine triphosphate; hemolysis; protein modification; antioxidant enzymes Introduction:

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Red blood cell storage and cell morphology

Abstract: Morphology parameters suggest that significant alterations to RBC morphology over storage duration occur soon after the 14th day of storage, as to become significant enough within the 21st day.

Cited by 147 publications

References 33 publications

Multiphoton excited hemoglobin fluorescence and third harmonic generation for non-invasive microscopy of stored blood

Multiphoton excited hemoglobin fluorescence and third harmonic generation for non-invasive microscopy of stored blood

The mechanical properties of stored red blood cells measured by a convenient microfluidic approach combining with mathematic model

Effect of Free Radical Generating System- Adenosine Triphosphate/Ferrous Sulphate/Hydrogen Peroxide on Human Erythrocytes

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