Red blood cell Thomsen‐Friedenreich antigen expression and galectin‐3 plasma concentrations in Streptococcus pneumoniae–associated hemolytic uremic syndrome and hemolytic anemia

Abstract

The results indicate high levels of neuraminidase activity and desialylation in both P-HUS and P-HA patients. T-antigen activation is more sensitive than DAT for P-HUS diagnosis. Combining PNA and SBA lectins is needed to improve the specificity of T-antigen activation. High concentrations of Gal-3 in P-HUS patients suggest that Gal-3 may contribute to the pathogenesis of P-HUS.

Severe hemolysis after plasma transfusion in a neonate with necrotizing enterocolitis, Clostridium perfringens infection, and red blood cell T‐polyagglutination

“…Negative DAT results have been reported in all cases of intravascular hemolysis associated with NEC and T activation to date for which DAT results were reported . By contrast, positive DAT with anti‐C3d were reported in a case of T polyagglutination and intravascular hemolysis in an adult patient, and in a number of cases of T activation associated with pneumococcal hemolytic and uremic syndrome (P‐HUS) . In the first case, eluates from the patient's RBCs tested negative.…”

“…Second, not only does the neuraminidase expose the T antigen, but it may also strongly decrease the amount of sialic acid on the surfaces of the RBCs . Sialic acids are increasingly recognized as key factors protecting host cells against complement attack .…”

Minipool Caprylic Acid Fractionation of Plasma Using Disposable Equipment: A Practical Method to Enhance Immunoglobulin Supply in Developing Countries

“…The content in IgA does not constitute a risk for primary immunodeficient patients who cannot develop anti-IgA. The preparation should not be infused to IgA-deficient patients, although such risks were recently highlighted as being not evidence-based in many patients [22]. Residual proteins included albumin, alpha-1, alpha-2, and bêta-proteins.…”

Sweeteners for factor H

“…That some patients with these latter forms of HUS respond to eculizumab supports the notion that loss of specific factor H-binding sialic acids might contribute to the tissue damage. 8 Indeed, in the context of factor H, stress-induced changes in sialic acid amount and composition may impact …”

“…In some cases, a clonal relationship to the primary malignancy has been established using immunoglobulin gene rearrangement studies, BRAF mutational analysis, or another translocation or chromosomal abnormality between the primary and secondary malignancies. 8 The R group, which consists in large part of Rosai-Dorfman disease, is usually straightforward diagnostically because of the characteristic cytomorphologic and immunophenotypic properties of the histiocytes. However, caution should be exercised in central nervous system disease, in which the clinical and radiographic appearance can simulate meningioma, and in cases with large numbers of IgG4 1 plasma cells, in which IgG4-related disease must be excluded.…”