Red blood cell (RBC) volume can be independently determined in vivo in humans using RBCs labeled at different densities of biotin

Mock, Donald M.; Matthews, Nell I.; Zhu, Shan; Burmeister, Leon F.; Zimmerman, M. Bridget; Strauss, Ronald G.; Schmidt, Robert L.; Nalbant, Demet; Cress, Gretchen A.; Widness, John A.

doi:10.1111/j.1537-2995.2010.02770.x

Cited by 27 publications

(60 citation statements)

References 26 publications

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“…Selection of the 6 μg/ml BioRBC density was based on our prior autologous RCS data in adults supporting the current study’s hypothesis that the lowest BioRBC densities accurately track true RBC survival (11). For 18 μg/ml BioRBC density, significant post hoc differences with the 6 μg/ml BioRBC reference group were detected at weeks 4, 6, 7, and 8 but not at week 5 (Table 3); For 54 μg/ml, statistically significant post hoc differences were observed at week 2 and thereafter except for at week 5.…”

Section: Resultssupporting

confidence: 58%

Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen–mismatched RBCs

et al. 2013

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BackgroundAnemia, a common condition among critically ill premature infants, is affected by red blood cell (RBC) survival (RCS). We hypothesized that transfused allogeneic Kidd antigen mismatched RBCs would demonstrate the same concurrent RCS tracking as RBCs multi-labeled at separate, discrete low densities with biotin (BioRBCs).MethodsAllogeneic RBCs from adult donors were labeled at four biotin densities, mixed, and transfused into 17 anemic premature infants. Nine of the donors and neonates were Kidd antigen mismatched. Serial post-transfusion blood samples were assayed for up to eight weeks by flow cytometry to track the survival of the proportions of Kidd antigen mismatched and biotinylated RBCs.ResultsUsing linear mixed modeling to compare results, RCS of the three lowest BioRBC densities was similar to RCS by Kidd antigen mismatch and to one another. RCS of RBCs labeled at the highest BioRBC density was shortened.ConclusionsRCS of different populations of RBCs can be tracked concurrently and reliably using the three lowest BioRBC densities. Although comparable RCS results can be achieved using Kidd antigen mismatches, BioRBCs are preferred for investigating neonatal anemias because biotin labeling of both allogeneic and autologous RBCs is possible.

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Section: Resultssupporting

confidence: 58%

Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen–mismatched RBCs

et al. 2013

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“…For example, contrary to previous understanding and similar to adult studies [49], blood volume normalized by body weight is about 70 mL/kg [6]. Also, the post-transfusion time during which a sample for determination of RCV is accurate ranges from as little as 20 min to at least 90 min.…”

Section: Clinical and Biological Applications Of The Biotin Methodsmentioning

confidence: 72%

“…The method described below was developed jointly by the Iowa, Arkansas, and Cincinnati groups and is being applied in all three locations with minor variations [5, 6, 49, 50]. All are adaptations of the method of Cavill et al who reported the first human application of the biotin RBC labeling method to measure RCV in adults [51].…”

Section: Methods For Biotin Rbc Labelingmentioning

confidence: 99%

“…In adults, 51 Cr has been used as the reference method to show equivalency using either one or two biotin densities of BioRBC [5, 53]. Results using four densities of BioRBC (6, 18, 54, and 162 μg/mL) further validate the method by demonstrating that the four densities of BioRBC concurrently administered yield equivalent RCV results [49]; if the density of biotin labeling had significantly reduced immediate BioRBC survival, one would have predicted RCV differences among the four labels. Moreover, in infants, clinical transfusion with allogeneic RBC with both minor RBC antigen differences and differences in hemoglobin species (HbA in adult donor blood and HbF in infant autologous blood) allowed comparison to two reference methods; in very low birth weight (VLBW) infants weighing <1.5 kg at birth, equivalent RCV values were observed [6, 56].…”

Section: In Vivo Validation Of the Biotin Methodsmentioning

confidence: 99%

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Measurement of Posttransfusion Red Cell Survival With the Biotin Label

Mock

Widness

Veng‐Pedersen

et al. 2014

Transfusion Medicine Reviews

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The goal of this review is to summarize and critically assess information concerning the biotin method to label red blood cells (RBC) for use in studies of RBC and transfusion biology — information that will prove useful to a broad audience of clinicians and scientists. A review of RBC biology, with emphasis on RBC senescence and in vivo survival is included, followed by an analysis of the advantages and disadvantages of biotin labeled RBC (BioRBC) for measuring circulating RBC volume, post-transfusion RBC recovery, RBC lifespan, and RBC age-dependent properties. The advantages of BioRBC over 51Cr RBC labeling, the current reference method, are discussed. Because the biotin method is straightforward and robust, including the ability to follow the entire lifespans of multiple RBC populations concurrently in the same subject, BioRBC offers distinct advantages for studying RBC biology and physiology, particularly RBC survival. The method for biotin labeling, validation of the method, and application of BioRBCs to studies of sickle cell disease, diabetes, and anemia of prematurity are reviewed. Studies documenting the safe use of BioRBC are reviewed; unanswered questions requiring future studies, remaining concerns, and regulatory barriers to broader application of BioRBC including adoption as a new reference method are also presented.

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“…For samples with slightly overlapping fluorescent intensity histograms, mixture modeling was used to calculate the proportion of cells from each RBC population. The lower limit of quantitation for individual BioRBC density peaks is 0.06% of total RBC 12 .…”

Section: Methodsmentioning

confidence: 98%

Autologous Infant and Allogeneic Adult Red Cells Demonstrate Similar Concurrent Post-Transfusion Survival in Very Low Birth Weight Neonates

Widness

Kuruvilla

Mock

et al. 2015

The Journal of Pediatrics

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Objective Based on the hypothesis that neonatal autologous red blood cell (RBC) survival (RCS) is substantially shorter than adult RBC, we concurrently tracked the survival of transfused biotin-labeled autologous neonatal and allogeneic adult RBC into ventilated, very low birth weight (VLBW) infants. Study design RBC aliquots from the first clinically ordered, allogeneic adult RBC transfusion and from autologous infant blood were labeled at separate biotin densities (BioRBC) and transfused. Survival of these BioRBCs populations were concurrently followed over weeks by flow cytometric enumeration using leftover blood. Relative tracking of infant autologous and adult allogeneic BioRBC was analyzed by linear mixed modeling of batched weekly data. When possible, Kidd antigen (Jka and Jkb) mismatches between infant and donor RBCs were also used to track these two populations. Results Contrary to our hypothesis, concurrent tracking curves of RCS of neonatal and adult BioRBC in 15 study infants did not differ until week 7, after which neonatal RBC survival became shortened to 59% to 79% of adult enumeration values for uncertain reasons. Analysis of mismatched Kidd antigen RBC showed similar results, thus confirming that BioRBC tracking is not perturbed by biotin RBC labeling. Conclusions This study illustrates the utility of multi-density BioRBC labeling for concurrent measurement of RCS of multiple RBC populations in vivo. The similar RCS results observed for neonatal and adult BioRBCs transfused into VLBW infants provides strong evidence that the circulatory environment of the newborn infant—not intrinsic infant-adult RBC differences—is the primary determinant of erythrocyte survival. Trial registration Clinicaltrials.gov: NCT 00731588.

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Red blood cell (RBC) volume can be independently determined in vivo in humans using RBCs labeled at different densities of biotin

Cited by 27 publications

References 26 publications

Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen–mismatched RBCs

Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen–mismatched RBCs

Measurement of Posttransfusion Red Cell Survival With the Biotin Label

Autologous Infant and Allogeneic Adult Red Cells Demonstrate Similar Concurrent Post-Transfusion Survival in Very Low Birth Weight Neonates

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